Dataset: coral-light expt.- protein

Name: Protein measurements from coral-light experiments (Dufault, 2013, JEB), Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project)
Published: 2014-08-30
Keywords: oceans

Cite This Dataset

Spatial Extent: N:21.9382 E:120.746 S:21.9382 W:120.746

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Program:

Long Term Ecological Research network (LTER)

Principal Investigator:

Peter J. Edmunds (California State University Northridge, CSUN)

Student:

Aaron M. Dufault (California State University Northridge, CSUN)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2014-08-30

Version Date:

2014-08-30

Validated:

Yes

Current State:

Final no updates expected

Protein measurements from coral-light experiments (Dufault, 2013, JEB), Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project)

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Description

The effect of light and PCO2 on the calcification and survival of Pocillopora damicornis recruits settled from larvae
released in southern Taiwan was tested.

These data include the protein measurements for each light treatment in each of the tanks over the duration of the experiment.

These data were published in Aaron M Dufault, Aaron Ninokawa, Lorenzo Bramanti, Vivian R Cumbo, Tung-Yung Fan, Peter J Edmunds (2013) The role of light in mediating the effects of ocean acidification on coral calcification. Journal of Experimental Biology 216: 1570-1577. doi:10.1242/jeb.080549

Download complete data for this publication (Excel file)

Methods & Sampling

In March 2011and June 2012, recruits were incubated at 31, 41, 70, 122 and 226 µmol photons m-2 s-1 under ambient (493 µatm) and high PCO2 (878 µatm). After 5 days, calcification was measured gravimetrically and survivorship estimated as the number of living recruits.

Three recruits per tile were harvested for protein analysis and frozen. Frozen recruits were solubilized in 0.1 mol/liter NaOH with sonication (15 s at 10% amplitude) and heating (5 h at 50°C), then neutralized with 1 mol/liter HCl (to pH 7.0-7.5) and were processed in triplicate. Protein concentration was determined using the BioRad protein assay (BioRad Laboratories, Hercules, CA, USA) scaled to microtiter plates with absorbance at 595 nm read on a 96-well plate spectrophotometer (Synergy H4 Hybrid Reader, Biotek, Winooski, VT, USA; the assay was calibrated using BSA).

Data Processing Description

BCO-DMO processing notes:

- added conventional header with dataset name, PI name, version date, reference information
- added lab, lat, lon, expt columns
- renamed parameters to BCO-DMO standard
- sorted by expt, treatment_pCO2, tank, treatment_light, tile, spat

Data Files

File
protein.csv (Comma Separated Values (.csv), 8.51 KB) MD5:19b1e29ccd7a7803da0df685555a218a Primary data file for dataset ID 527782

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0844785

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
lab_Edmunds_NMMBA		2010-03-18	Natl Museum Mar. Bio. and Aquar. Taiwan	Peter J. Edmunds (Lead Principal Investigator)	data info

Instruments

Spectrophotometer

Supplied Name: spectrophotometer

Supplied Description:

96-well plate spectrophotometer: Synergy H4 Hybrid Reader, Biotek, Winooski, VT, USA; the assay was calibrated using BSA

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
lab	laboratory	unitless	laboratory
lat	latitude; north is positive	degrees	lat
lon	longitude; east is positive	degrees	lon
expt	experiment identification number	unitless	exp_id
tank	tank identification number	unitless	tank
treatment_pCO2	relative partial pressure of carbon dioxide (pCO2) target for treatment	unitless	pCO2
treatment_light	light level for treatment	mol photons/m2/s	irradiance
tile	setting tile id number	unitless	replicate
spat	individual larval coral id number	unitless	individual
protein_total	total protein concentration for the grouped sample	milligrams	no_bcodmo_term
protein_stdev	grouped sample protein standard deviation	milligrams	std_dev

Status message

Database

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Dataset: coral-light expt.- protein