Dataset: HPLC_pigments
Deployment: TT050

Pigments, HPLC method, sampled from bottle casts

Principal Investigator:

Robert R. Bidigare (University of Hawai'i, UH)

BCO-DMO Data Manager:

Cynthia L. Chandler (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

U.S. JGOFS Arabian Sea (Arabian Sea)

Version:

May 8, 2001

Deployment Synonyms:

TTN050, TN050, TTN-050

Version Date:

2001-05-08

Data URL:

https://www.bco-dmo.org/dataset-deployment/450647/data

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Description

Pigments, HPLC method, sampled from bottle casts

Methods & Sampling

Dataset acquisition description

See Platform deployments for cruise specific documentation

Deployment-specific acquisition description

   PI:        Robert R. Bidigare
   of:        University of Hawaii
   dataset:   Pigments, HPLC method, sampled from bottle casts
   dates:     August 18, 1995 to September 13, 1995
   location:  N: 22.4688  S: 9.9991  W: 57.3004  E: 68.7494
   cruise:    TTN-050, Arabian Sea Process cruise #5 (Late SW Monsoon)
   ship:      R/V Thomas Thompson

Data Processing Description

Dataset Processing Description

See Platform deployments for cruise specific documentation

Deployment-specific processing description

<pre>
<h2>HPLC Pigment methods</h2>

<b>Method by Wright et al (Mar. Ecol. Prog. Ser. 1991, 77:183-196) CHLA1,
CHLA2, CHLB1 and CHLB2 estimated following the method of Latasa et al
(Mar. Chem. 1996, 51:315-324)</b>
<hr>

Pigment data for P2 & P5:

A comparison of the TURNER-determined chlorophyll a concentrations with the
HPLC-determined TOTCHLA concentrations (monovinyl chlorophyll a + divinyl
chlorophyll a + monovinyl chlorophyllide a; units = ng Chl a
equivalents/L) was performed for Process Cruise #2 (TTN-045) and Process
Cruise #5 (TTN-050). While good correlations were obtained for both
cruises, the slope obtained for Process Cruise #5 was significantly
different from 1 (i.e., TURNER > HPLC). This difference was probably
caused by the presence of Chl a-related pigments during Process Cruise #5.
Thus, we recommend that whenever possible use the HPLC pigment data and not
the TURNER pigment data. If HPLC data is not available for a given cast,
we further recommend that you use the following equations to transform the
TURNER data into HPLC-equivalent concentrations (cf., Babin, M., A. Morel,
H. Claustre, A. Bricaud, Z. Kolber and P.G. Falkowski. 1996. Nitrogen-
and irradiance-dependent variations of the maximum quantum yield of carbon
fixation in eutrophic, mesotrophic and oligotrophic marine systems.
Deep-Sea Research, in press).

Results of geometric mean regression analyses (reduced major axis):

Y = HPLC TOTCHLA (monovinyl chlorophyll a + divinyl chlorophyll a +
monovinyl chlorophyllide a), units = ng Chl a equivalents/L

X = TURNER chlorophyll a (it is necessary to convert the Turner Chl a
concentrations in the Arabian Sea data base from mg/m3 to ng/L by
multiplying concentrations by 1000)

(1) Process Cruise #2 (TTN-045)

HPLC TOTCHLA = TURNER*(0.975) + 4.833 (r = 0.9822, n = 146)

(2) Process Cruise #5 (TTN-050)

HPLC TOTCHLA = TURNER*(0.708) + 12.881 (r = 0.9772, n = 575)

Robert R. Bidigare
Department of Oceanography
University of Hawaii
Honolulu, HI 96822
808-956-6567 (voice mail)
808-956-9516 (fax)
</pre>

More information about this dataset deployment

Funding

Award Number	Funding Source
unknown Arabian Sea NASA	National Aeronautics & Space Administration
unknown Arabian Sea NSF	National Science Foundation

Instruments

Niskin bottle

Supplied Name: Niskin Bottle

Supplied Description:

CTD/Niskin Rosette bottles

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Trace Metal Bottle

Supplied Description:

Trace Metal (TM) Rosette bottles

Instrument Type

Generic Name: Trace Metal Bottle

Acronym: TM Bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/30/

Generic Description:

Trace metal (TM) clean rosette bottle used for collecting trace metal clean seawater samples.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
event	event number from event log		event
sta_std	Arabian Sea standard station identifier		no_bcodmo_term
sta	station number from event log		no_bcodmo_term
cast	CTD or TM cast number from event log		cast
bot	CTD or TM bottle number		no_bcodmo_term
depth_n	nominal sample depth	meters	no_bcodmo_term
chlide_a	chlorophyllide a	nanogram/liter	no_bcodmo_term
chl_c3	chlorophyll c3	nanogram/liter	chl_c3
chl_c	sum of chl_c1, chl_c2 and Mg 3,8 divinyl-pheophorphyrin a5	nanogram/liter	chl_c
peridinin	peridinin	nanogram/liter	no_bcodmo_term
fucox_but	19'-butanoyloxyfucoxanthin	nanogram/liter	no_bcodmo_term
fucox	fucoxanthin	nanogram/liter	no_bcodmo_term
fucox_hex	19'-hexanoyloxyfucoxanthin	nanogram/liter	no_bcodmo_term
violax	violaxanthin	nanogram/liter	no_bcodmo_term
diadinox	diadinoxanthin	nanogram/liter	no_bcodmo_term
allox	alloxanthin	nanogram/liter	no_bcodmo_term
diatox	diatoxanthin	nanogram/liter	no_bcodmo_term
zeax	zeaxanthin	nanogram/liter	no_bcodmo_term
carotene	sum of alpha and beta carotene	nanogram/liter	no_bcodmo_term
chl_b2	divinyl chlorophyll b	nanogram/liter	no_bcodmo_term
chl_b1	monovinyl chlorophyll b	nanogram/liter	no_bcodmo_term
chl_a2	divinyl chlorophyll a	nanogram/liter	no_bcodmo_term
chl_a1	monovinyl chlorophyll a	nanogram/liter	no_bcodmo_term
chl_b_tot	sum of chl_b1 and chl_b2	nanogram/liter	no_bcodmo_term
chl_a_tot	sum of chl_a1, chl_a2, chlide_a, chl_a1' and allomerized chla	nanogram/liter	no_bcodmo_term
neox	neoxanthin	nanogram/liter	no_bcodmo_term
chl_c4_1	phytolated chl_c type 1	nanogram/liter	no_bcodmo_term
chl_c4_2	phytolated chl_c type 2	nanogram/liter	no_bcodmo_term
pig_unid	pigments, sum of all major unidentified	nanogram/liter	no_bcodmo_term
cis_fucox	Cis-fucoxanthin	nanogram/liter	no_bcodmo_term
cis_hex	Cis-19'-hexanoyloxyfucoxanthin	nanogram/liter	no_bcodmo_term
prasinox	Prasinoxanthin	nanogram/liter	no_bcodmo_term
lutein	Lutein	nanogram/liter	no_bcodmo_term
carotene_a	alpha-carotene	nanogram/liter	no_bcodmo_term
carotene_b	beta-carotene	nanogram/liter	no_bcodmo_term
replicate	yes - indicates replicate measurements		replicate
chlide_b	chlorophyllide b	nanogram/liter	no_bcodmo_term
chl_a1_prime	chlorophyll a1'	nanogram/liter	no_bcodmo_term

Database

Contribute Data

Dataset: HPLC_pigments
Deployment: TT050

Dataset acquisition description

Deployment-specific acquisition description

Dataset Processing Description

Deployment-specific processing description

Database

Contribute Data

Dataset: HPLC_pigmentsDeployment: TT050

Dataset acquisition description

Deployment-specific acquisition description

Dataset Processing Description

Deployment-specific processing description

Dataset: HPLC_pigments
Deployment: TT050