Dataset: microzooplankton
Deployment: TT054

Microzooplankton abundance and carbon biomass

Principal Investigator:

Dr Marcia Gowing (University of California-Santa Cruz, UCSC)

Co-Principal Investigator:

David L. Garrison (University of California-Santa Cruz, UCSC)

BCO-DMO Data Manager:

Cynthia L. Chandler (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

U.S. JGOFS Arabian Sea (Arabian Sea)

Version:

October 2, 2001

Deployment Synonyms:

TTN054, TN054, TTN-054

Version Date:

2001-10-02

Data URL:

https://www.bco-dmo.org/dataset-deployment/450698/data

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Description

Microzooplankton abundance and carbon biomass

Methods & Sampling

Dataset acquisition description

See Platform deployments for cruise specific documentation

Deployment-specific acquisition description

   PI:              David Garrison and Marcia Gowing
   of:              University of California - Santa Cruz
   dataset:         Microzooplankton abundance and carbon biomass
   dates:           December 05, 1995 to December 24, 1995
   location:        N: 19.2079  S: 9.9674  W: 58.1373  E: 67.1739
   project/cruise:  Arabian Sea/TTN-054 - Process Cruise 7 (Early NE Monsoon)
   ship:            Thomas Thompson
 
   
   Note:  Abundance of nanoflagellates represent the 2-20 micron size class.
   All other abundances represent the 20-200 micron range.
 
   DMO Note on multiple-bottle events

Data Processing Description

Dataset Processing Description

David Garrison and Marcia Gowing

Microzooplankton Methods

Samples for microzooplankton biomass were obtained by taking ~ 2-liter
aliquots from standard hydrographic casts. Heterotrophic flagellates
and some nano-planktonic non-loricate ciliates were examined by
epifluorescence microscopy on samples preserved with approximately 0.5%
glutaraldehyde, concentrated on 0.8 micron, black Nuclepore filters,
and stained with the fluorochromes DAPI (Coleman 1980) and proflavin
(Haas 1982) following the protocol outlined by Verity and Sieracki
(1993).  Whole water samples were preserved with buffered
paraformaldehyde.  Larger heterotrophic dinoflagellates, and most of
the ciliates were enumerated from 50 or 100 ml of these preserved
samples that were settled and counted with an inverted microscope.
Biomass was estimated by converting cell volumes (calculated from
measurements of cell dimensions) using the relationship ((Log10 C =
0.94 (log10 V)-0.60); with C representing carbon as picograms per cell
and V representing total cell volume in cubic microns) for flagellates
(Eppley et al. 1970), and the relationship carbon per cell = 0.16
pgC/cubic micron (Stoecker et al. 1994).

Coleman, A.W.  1980.  Enhanced staining of bacteria in natural
environments by fluorochrome staining of DNA.  Limnol. Oceanogr.
25:948-951.

Eppley, R.W., F.M.H. Reid, J.D.H. Strickland.  1970.  The ecology of
the plankton off La Jolla, California, in the period April through
September, 1967.  (ed. J.D.H. Strickland), pt. III, Estimates of
phytoplankton crop size, growth rate and primary production.  Bull.
Scripps Inst. Oceanogr.  17:33-42.

Haas, L.W.  1982.  Improved epifluorescence microscopy for observing
planktonic microorganisms.  Ann. Inst. Oceanogr. Paris.  58S:261-266.

Stoecker, D.K., D.J. Gifford, and M. Putt.  1994.  Preservation of
marine planktonic ciliates: losses and cell shrinkage during fixation.
Mar. Ecol. Prog. Ser.  110:293-299.

Verity, P.G. and M.E.Sieracki.  1993.  Use of color image analysis and
epifluorescence microscopy to measure plankton biomass. In: (Kemp,
P.F., B.F. Sherr,E.B. Sherr, and J.C. Cole, eds.)  Aquatic Microbial
Ecology.  pp. 327-338.  Lewis Publishers, Boca Raton.

Deployment-specific processing description

<pre>
David Garrison and Marcia Gowing

Microzooplankton Methods

Samples for microzooplankton biomass were obtained by taking ~ 2-liter
aliquots from standard hydrographic casts. Heterotrophic flagellates
and some nano-planktonic non-loricate ciliates were examined by
epifluorescence microscopy on samples preserved with approximately 0.5%
glutaraldehyde, concentrated on 0.8 micron, black Nuclepore filters,
and stained with the fluorochromes DAPI (Coleman 1980) and proflavin
(Haas 1982) following the protocol outlined by Verity and Sieracki
(1993). Whole water samples were preserved with buffered
paraformaldehyde. Larger heterotrophic dinoflagellates, and most of
the ciliates were enumerated from 50 or 100 ml of these preserved
samples that were settled and counted with an inverted microscope.
Biomass was estimated by converting cell volumes (calculated from
measurements of cell dimensions) using the relationship ((Log10 C =
0.94 (log10 V)-0.60); with C representing carbon as picograms per cell
and V representing total cell volume in cubic microns) for flagellates
(Eppley et al. 1970), and the relationship carbon per cell = 0.16
pgC/cubic micron (Stoecker et al. 1994).

Coleman, A.W. 1980. Enhanced staining of bacteria in natural
environments by fluorochrome staining of DNA. Limnol. Oceanogr.
25:948-951.

Eppley, R.W., F.M.H. Reid, J.D.H. Strickland. 1970. The ecology of
the plankton off La Jolla, California, in the period April through
September, 1967. (ed. J.D.H. Strickland), pt. III, Estimates of
phytoplankton crop size, growth rate and primary production. Bull.
Scripps Inst. Oceanogr. 17:33-42.

Haas, L.W. 1982. Improved epifluorescence microscopy for observing
planktonic microorganisms. Ann. Inst. Oceanogr. Paris. 58S:261-266.

Stoecker, D.K., D.J. Gifford, and M. Putt. 1994. Preservation of
marine planktonic ciliates: losses and cell shrinkage during fixation.
Mar. Ecol. Prog. Ser. 110:293-299.

Verity, P.G. and M.E.Sieracki. 1993. Use of color image analysis and
epifluorescence microscopy to measure plankton biomass. In: (Kemp,
P.F., B.F. Sherr,E.B. Sherr, and J.C. Cole, eds.) Aquatic Microbial
Ecology. pp. 327-338. Lewis Publishers, Boca Raton.
</pre>

More information about this dataset deployment

Funding

Award Number	Funding Source
unknown Arabian Sea NSF	National Science Foundation

Instruments

Niskin bottle

Supplied Name: Niskin Bottle

Supplied Description:

CTD/Niskin Rosette bottles.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Trace Metal Bottle

Supplied Description:

Trace Metal (TM) Rosette bottles

Instrument Type

Generic Name: Trace Metal Bottle

Acronym: TM Bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/30/

Generic Description:

Trace metal (TM) clean rosette bottle used for collecting trace metal clean seawater samples.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
event	event number from event log		event
sta_std	Arabian Sea standard station identifier		no_bcodmo_term
sta	station number from event log		no_bcodmo_term
cast	CTD cast number		cast
cast_type	CTD=CTD rosette; TM=TM rosette		no_bcodmo_term
bot	CTD bottle number - see note, please		no_bcodmo_term
depth_n	nominal depth	meters	no_bcodmo_term
nanoflag_het	heterotrophic nanoflagellate abundance (excluding dinoflagllates)	cells/liter	no_bcodmo_term
nanoflag_het_C	heterotrophic nanoflagellate biomass (excluding dinoflagllates)	micrograms C/liter	no_bcodmo_term
dino_het	heterotrophic dinoflagellate abundance	cells/liter	no_bcodmo_term
dino_het_C	heterotrophic dinoflagellate biomass	micrograms C/liter	no_bcodmo_term
ciliates_n	non-loricate ciliate abundance (including plastidic oligotrichs)	cells/liter	no_bcodmo_term
ciliates_n_C	non-loricate ciliate biomass (including plastidic oligotrichs)	micrograms C/liter	no_bcodmo_term
tint	tintinnid (ciliate) abundance	cells/liter	no_bcodmo_term
tint_C	tintinnid (ciliate) biomass	micrograms C/liter	no_bcodmo_term

Database

Contribute Data

Dataset: microzooplankton
Deployment: TT054

Dataset acquisition description

Deployment-specific acquisition description

Dataset Processing Description

Deployment-specific processing description

Database

Contribute Data

Dataset: microzooplanktonDeployment: TT054

Dataset acquisition description

Deployment-specific acquisition description

Dataset Processing Description

Deployment-specific processing description

Dataset: microzooplankton
Deployment: TT054