Dataset: HPLC_MOTE
Deployment: HG93_CARIACO

Water for the HPLC filters was collected from the first cast done during the CARIACO core cruises, carried out around 5 am local time. During periods of low primary production, 2000 ml. of water were vacuum filtered through a 47mm GF/F filter pad. During periods of high primary production, water was filtered until the filter was clogged. The volume of water filtered was annotated. Eight depths were sampled: 1, 7, 15, 25, 35, 55, 75 and 100 m. If a chlorophyll maximum was found at a different depth, this depth was also sampled. A duplicate was taken at one of these depths (usually at 1m), for QA/QC assessment. Filters were carefully folded in half, stored in aluminum foil and refrigerated until reaching shore. Once back on shore, they were stored frozen at -40°C. Filters were transported from Margarita Island to USF and stored frozen (at -40°C) until they were shipped in a liquid-nitrogen storage to the laboratory that performed the HPLC analysis. The CARIACO samples of HPLC were analyzed by: Bermuda Biological Research Station, BBRS (CAR-002 to CAR-030); MOTE Marine Laboratory & Aquarium (CAR-031 to CAR-068), Horn Point Laboratory, HPL (CAR-124 to CAR-175); and NASA Goddard Space Flight Center, GSFC (CAR-176 to CAR-232). Analysis at HPL and GSFC were carried out according to the method described in Hooker et al. (2005), Chapter 5. The HPLC parameter names as reported in this dataset correspond to the pigment names as defined in the NASA SeaWiFS Bio-optical Archive and Storage System (SeaBASS) (https://seabass.gsfc.nasa.gov/wiki/stdfields). Fluorometric estimations of chlorophyll-a and phaeopigments concentrations were done with a Turner Design10-AU-005 Fluorometer using standard methods (Holm-Hansen et al., 1965; Falkowski and Kiefer, 1985). The detailed methodology for the determination of Chlorophyll a and Phaeopigments can be found in the HANDBOOK OF METHODS FOR THE ANALYSIS OF OCEANOGRAPHIC PARAMETERS AT THE CARIACO TIME-SERIES STATION,  edited by Astor, Lorenzoni and Scranton (2011).  (https://seabass.gsfc.nasa.gov/archive/USF/FMK/CARIACO/2015/documents/CARIACO_Methods_Manual.pdf)