Dataset: nutrients and ARBS sponge disease
Deployment: lab_Olson_ARBS

chlorophyll-a, cyanobacteria, fluorescence and total protein in healthy and diseased sponges

View Data: For data, See Dataset Metadata Page: https://www.bco-dmo.org/dataset/3609

Principal Investigator:

Julie Olson (University of Alabama-Tuscaloosa, UA/Tuscaloosa)

Co-Principal Investigator:

Deborah J. Gochfeld (University of Mississippi (Ole Miss), UM-MMRI)

Robert W. Thacker (University of Alabama at Birmingham, UA/Birmingham)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Aplysina Red Band Syndrome: Investigating the etiology, pathogenesis, and ecology of an emerging marine disease (Etiology of ARBS sponge disease)

Current State:

Final no updates expected

Version Date:

2012-01-25

Expand/Collapse All

Description

To assess the interacting effects of nutrients and disease on Aplysina cauliformis, we provided nutrient enrichment to healthy and diseased sponges in a factorial experiment at LSI in the summer of 2009. Impacts on ARBS virulence (rate of lesion growth) and host response (growth and physiology of both the sponge and its cyanobacterial symbionts, Synechococcus spongiarum) were assessed.

Methods & Sampling

Dataset acquisition description

Sponge samples were collected from patch reefs near the Perry Institute for Marine Science, Lee Stocking Island, Bahamas.

Data Processing Description

Dataset Processing Description

Units for measurements of the analyses:
chla: micrograms of chlorophyll-a per milligram of sponge wet weight;
cyanobacteria: cells per 2886 micometers viewing area;
fluorescent yield: no units;
total protein: micrograms protein per mg sponge tissue (lyophilized prior to extraction)

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0726944	NSF Division of Ocean Sciences
OCE-0727833	NSF Division of Ocean Sciences
OCE-0727996	NSF Division of Ocean Sciences

Instruments

None available yet

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
analysis_type	chlophyll-a;cyanobacteria; fluorescence; total protein measured	text	no_bcodmo_term
condition	initial condition of sponge specimen; healthy or diseased	text	no_bcodmo_term
nutrients_treatment	experimental treatment; enriched or none	text	no_bcodmo_term
sponge_id	identification number of the sponge specimen used in the experiment	1 to 12	no_bcodmo_term
rack_id	rack number: sponges were placed on racks for experimental exposure with 2 sponges per rack	1 to 24	no_bcodmo_term
initial	initial measurement of the analysis. chla: micrograms of chlorophyll-a per milligram of sponge wet weight;cyanobacteria: cells per 2886 micometers viewing area;fluorescent yield: no units;total protein: micrograms protein per mg sponge tissue (lyophilized prior to extraction)	number	no_bcodmo_term
final	final measurement of the analysis; same units as initial measurements	number	no_bcodmo_term

Database

Contribute Data

Dataset: nutrients and ARBS sponge disease
Deployment: lab_Olson_ARBS

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: nutrients and ARBS sponge diseaseDeployment: lab_Olson_ARBS

Dataset acquisition description

Dataset Processing Description

Dataset: nutrients and ARBS sponge disease
Deployment: lab_Olson_ARBS