Dataset: HPLC_pigments
Deployment: LIS0507

Diagnostic accessory pigment data analyzed by HPLC

Principal Investigator:

George McManus (University of Connecticut, UConn - Avery Point)

Co-Principal Investigator:

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Testing hypotheses about diversity, gene flow, and effective population size in marine planktonic ciliates (CiliateDivGenePop)

Current State:

Final no updates expected

Version:

11 May 2012

Version Date:

2012-05-11

Data URL:

https://www.bco-dmo.org/dataset-deployment/455184/data

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Description

Accessory pigment analysis data for samples taken from 6 stations in Long Island Sound, including fucox, allox, lutein, gyrox, carotene, pheophorbide a, pheophytin a, chlorophyll, and others.

Related files and references:
Doherty, M, M Tamura, BA Costas, ME Ritchie, GB McManus, and LA Katz. 2010. Ciliate Diversity and Distribution Across an Environmental and Depth Gradient in Long Island Sound, USA. Environmental Microbiology 12:886-898. doi:10.1111/j.1462-2920.2009.02133.x (PDF)

Methods & Sampling

Dataset acquisition description

Samples were collected on research cruise LIS0507 in the estuary of Long Island Sound on 01 June 2007. Sampling occurred at stations designed to capture the area where water exiting the Connecticut River forms a shallow plume of low-salinity water. For the HPLC analyses,100 ml samples were collected on glass fiber filters and extracted in acetone.

Data Processing Description

Dataset Processing Description

Samples were processed at Horn Point Lab (HPL). Samples were collected in a vacuum. Extraction in the lab took place on 06 November 2011. GF/F type filters of 25-mm diameter were used. Filters were stored at -70 degrees C prior to shipping to HPL. Pigment data were processed using CHEMTAX software.

BCO-DMO made the following modifications to the dataset: format of parameter names was changed to conform to BCO-DMO conventions; blanks were replaced with 'nd'.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0648711	NSF Division of Ocean Sciences

Instruments

High-Performance Liquid Chromatograph

Supplied Name: High Performance Liquid Chromatograph

Supplied Description:

HPLC pigment data was processed using CHEMTAX software.

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
cruiseid	Identifier that represents the research cruise.	dimensionless	cruiseid
year	Year the sample was collected, in YYYY format.	dimensionless	year
month_gmt	Month the sample was collected. Converted from text to GMT format (MM).	dimensionless	month_gmt
day_gmt	Day the sample was collected, in DD format.	dimensionless	day_gmt
yrday_gmt	Sequential day of the year on which the sample was collected.	dimensionless	yrday_gmt
station	Numeric identifier of the sample station.	dimensionless	station
sample	Unique identifier of the sample. Column originally named 'Original PI Sample Code'.	dimensionless	sample
sample_lab	Sample code assigned by the lab. Column originally named 'Horn Point Lab sample code'.	dimensionless	sample
sample_seq	Sequential sample number.	dimensionless	sample
site_descrip	Indicates if the sample site is located in ('in_plume') or out ('out_plume') of the plume. Column originally named 'In plume or out'.	dimensionless	site_descrip
lat	Latitude in decimal degrees.	decimal degrees	lat
lon	Longitude in decimal degrees. Negative indicates West.	decimal degrees	lon
pigment	Name of the pigment measured: chlide_a = chlorophyllide a p_phorbide = pheophorbide a (or pheide) fucox_but = 19-prime-butanoyloxyfucoxanthin fucox = fucoxanthin fucox_hex = 19-prime-hexanoyloxyfucoxanthin p_phytin = pheophytin a (or phaeophytin)	dimensionless	pigment_conc
conc	Concentration of the pigment in micrograms/Liter.	ug/L	pigment_conc
time_gmt	GMT time in HHMM format.	dimensionless	time_gmt
depth	Sample depth in meters.	meters	depth
vol_filt	Volume of water filtered, in milliliters.	mL	fluor_vol_filt
comments	Comments.	dimensionless	comment
vol_extr	Volume extracted, measured in milliliters.	mL	no_bcodmo_term

Database

Contribute Data

Dataset: HPLC_pigments
Deployment: LIS0507

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: HPLC_pigmentsDeployment: LIS0507

Dataset acquisition description

Dataset Processing Description

Dataset: HPLC_pigments
Deployment: LIS0507