Dataset: nutrients_particulate_Ant1
Deployment: NBP0601

Particulate nutrients from temp and iron experiments on Antarctic phyto and microzooplankton

Principal Investigator:

Dr Julie Rose (National Oceanic and Atmospheric Administration , NOAA-Milford)

Co-Principal Investigator:

David A. Hutchins (University of Southern California, USC)

BCO-DMO Data Manager:

Danie Kinkade (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Controls of Ross Sea Algal Community Structure (CORSACS)

Rising climatic temperatures impact on antarctic microzooplankton growth and grazing (Antarctic microzooplankton )

Deployment Synonyms:

CORSACS I

Data URL:

https://www.bco-dmo.org/dataset-deployment/505010/data

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Description

Experimental Design

Experiments were conducted during the CORSACS (Controls On Ross Sea Algal Community Structure) expedition in January 2006 to the Ross Sea, Antarctica, onboard the RVIB Nathaniel B. Palmer (cruise NBP-0601). Water was collected at 75.00S, 177.36E using a trace metal clean towed-intake surface water Teflon diaphragm pumping system (Bruland et al., 2005). Water was prescreened through acid-washed 200μm Nitex mesh to eliminate large zooplankton and collected into a 50-L mixing carboy. Collected water was gently mixed and dispensed into 12 4.5-L and 12 2.7-L acid washed trace metal clean clear polycarbonate bottles for incubation. Half of the bottles were spiked with 1.0nM FeCl3 (final concentration) at the beginning of the experiment. Bottles were incubated in two temperature controlled deck-board incubators (Feng et al., 2009; Hare et al., 2007). Incubators were screened to 18% of Io using two layers of neutral density filter. One incubator was kept at ambient temperature (0 deg C), while the temperature in the other was gradually increased to 4 deg C over the course of 24 h. Bottles were incubated for seven days. The 4.5-L bottles were sampled daily and the 2.7-L bottles were only sampled on the final day of the experiment. All sampling occurred under a laminar flow hood using trace metal clean techniques.

References

Bruland, K.W., E.L. Rue, G.J. Smith, and G.R. DiTullio. 2005. Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru. Marine Chemistry 93: 81-103.

Feng, Y., C.E. Hare, K. Leblanc, G.R. DiTullio, P.A. Lee, S.W. Wilhelm, J. Sun, J.M. Rose, N. Nemcek, I. Benner, and D.A. Hutchins. 2009. The effects of increased pCO2 and temperature on the North Atlantic Spring Bloom: I. The phytoplankton community and biogeochemical response. Marine Ecology Progress Series 388: 13-25.

Hare, C.E., K. Leblanc, G.R. DiTullio, R.M. Kudela, Y. Zhang, P.A. Lee, S.F. Riseman, and D.A. Hutchins. 2007. Consequences of increased temperature and CO2 for phytoplankton community structure in the Bering Sea. Marine Ecology Progress Series 352: 9-16.

Methods & Sampling

Dataset acquisition description

Particulate nutrient Sampling:

Total particulate carbon and nitrogen samples (150–250 mL) were filtered at low vacuum onto GF/F filters that had been precombusted at 450oC for 2 h. Filters were then dried at 60oC. Samples were analyzed with a Finnigan Delta Plus mass spectrometer and a Carlo Erba NA1500 elemental analyzer/Conflo II. Elemental compositions were measured using the mass 44 beam intensity (V) on the Delta Plus and calibrated against the mass 44 beam intensity of at least five standards also analyzed during each run of 40 samples. Total particulate phosphorus samples (100–200 mL) were gently filtered onto precombusted GF/F filters and rinsed with 2 ml 0.12 mol L−1 Na2SO4. Filters were placed overnight in precombusted (450oC, overnight) 20mL borosilicate glass scintillation vials with 2mL 0.017 mol L−1 MnSO4. Vials were covered with aluminum foil, dried at 95oC and stored dessicated until analysis. Total particulate phosphorus content was determined as follows: vials and filters were combusted at 450oC for 2 h, cooled, and 5mL 0.2 mol L−1 HCl was added to each vial. Vials were capped and heated to 80oC for 30 min to digest particulate organic phosphorus into inorganic phosphate, and digested samples were analyzed using the standard molybdate colorimetric method (Solorzano and Sharp, 1980). Biogenic silica samples (100–250 mL) were gently filtered onto 0.6μm polycarbonate filters, dried at 60oC and stored at room temperature until analysis. Samples were analyzed according to Brzezinski and Nelson (1995).

References:

Bruland, K.W., E.L. Rue, G.J. Smith, and G.R. DiTullio. 2005. Iron, macronutrients and diatom blooms in the Peru upwelling regime: brown and blue waters of Peru. Marine Chemistry 93: 81-103.
Brzezinski, M.A., and D.M. Nelson. 1995. The annual silica cycle in the Sargasso Sea near Bermuda. Deep-Sea Research I 42: 1215-1237.
Buck, K.N., M.C. Lohan, C.J.M. Berger, and K.W. Bruland. 2007. Dissolved iron speciation in two distinct river plumes and an estuary: implications for riverine iron supply. Limnology and Oceanography 52: 843-855.
Donat, J.R., and K.W. Bruland. 1988. Direct determination of dissolved cobalt and nickel in seawater by differential pulse cathodic stripping voltammetry preceded by adsorptive collection of cyclohexane-1,2,-dione dioxime complexes. Analytical Chemistry 60.
Ellwood, M., and C.M.G. Van den Berg. 2000. Zinc speciation in the Northeastern Atlantic Ocean. Marine Chemistry 68: 295-306.
Solorzano, L., and J.H. Sharp. 1980. Determination of total dissolved phosphorus and particulate phosphorus in natural waters. Limnology and Oceanography 25: 756-760.

Data Processing Description

Dataset Processing Description

BCO-DMO Processing Notes:

- File was sorted by treatment
- Added lat,lon values of original water sampling location to file
- Added BCO-DMO header lines
- Parameter names were edited to conform with BCO-DMO convention

More information about this dataset deployment

Funding

Award Number	Funding Source
PLR-0528715	NSF Antarctic Sciences

Instruments

CHN Elemental Analyzer

Supplied Name: CHN_EA

Supplied Description:

A Carlo Erba NA1500 elemental analyzer/Conflo II was used for analysis.

Instrument Type

Generic Name: CHN Elemental Analyzer

Acronym: CHN_EA

Generic Description:

A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.

Mass Spectrometer

Supplied Name: Mass Spec

Supplied Description:

A Finnigan Delta Plus mass spectrometer was used for analysis.

Instrument Type

Generic Name: Mass Spectrometer

Acronym: Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
lat	Latitude component of geographic position where water was sampled.	decimal degrees	lat
lon	Longitude component of geographic position where water was sampled.	decimal degrees	lon
treatment	Experimental conditions varied during the experiment. Four treatments were used: High temperature, high iron (HTHF); High temperature, low iron (HTLF); Low temperature, high iron (LTHF); Low temperature, low iron (LTLF)	dimensionless	treatment
day	Sampling day during experiment. The experiment was conducted during January, 2006.	dimensionless	no_bcodmo_term
bottle	Experimental bottle number.	dimensionless	no_bcodmo_term
carbon_partic	Total particulate carbon.	micromolar	TPC
silica_biogenic	Biogenic silica.	micromolar	Si_bio
nitrogen_partic	Total particulate nitrogen.	micromolar	TPN
phosphorus_partic	Total particulate phosphorus.	micromolar	TPP

Database

Contribute Data

Dataset: nutrients_particulate_Ant1
Deployment: NBP0601

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: nutrients_particulate_Ant1Deployment: NBP0601

Dataset acquisition description

Dataset Processing Description

Dataset: nutrients_particulate_Ant1
Deployment: NBP0601