Dataset: Symbiodinium_Seriatopora photosynthesis
Deployment: lab_Edmunds_NMMBA

Symbiodinium cell density, chlorophyll, respiration, photosynthetic yield in Seriatopora coral

Principal Investigator:

Peter J. Edmunds (California State University Northridge, CSUN)

Scientist:

Tung-Yung Fan (National Museum of Marine Biology and Aquarium, NMMBA)

Student:

Mr Chris B. Wall (Santa Monica College, SMC)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Moorea Coral Reef Long-Term Ecological Research site (MCR LTER)

The ecophysiological basis of the response of coral larvae and early life history stages to global climate change (Climate_Coral_Larvae)

Current State:

Final no updates expected

Version:

2014-08-12

Version Date:

2014-08-12

Data URL:

https://www.bco-dmo.org/dataset-deployment/522594/data

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Description

We hypothesized that ocean acidification would cause bleaching as defined by decreased photochemical efficiency, reduced photosynthetic capacity and efficiency, depressed chlorophyll a content, and lowered Symbiodinium densities and that these effects would be exacerbated with high temperature.

These data were published in C. B. Wall, T.-Y. Fan, P. J. Edmunds (2014) Ocean acidification has no effect on thermal bleaching in the coral. Coral Reefs 33:119-13. doi: http://dx.doi.org/10.1007/s00338-013-1085-2.

Download complete data for this publication (Excel file)

Methods & Sampling

Dataset acquisition description

Experimental design

Four treatments contrasted high and low temperature and pCO2:
Ambient temperature = 27.5 oC
Ambient pCO2 = 39.0 Pa
High temperature = 30.5 oC
High pCO2 = 86 Pa

Juvenile Seriatopora caliendrum were collected from Hobihu Reef, Nanwan Bay, Taiwan, and kept at the National Museum of Marine Biology and Aquarium (NMMBA), where they were placed randomly into the treatment tanks for incubations lasting 14 days. Corals were exposed to treatments in 8 tanks filled with filtered seawater. Treatments were maintained at a salinity of 33 and were monitored daily for temperature, salinity, irradiance, pH and carbonate chemistry.

Photochemical efficiency: The effects of temperature and pCO2 on photochemical efficiency were tested by measuring the maximum photochemical efficiency of open RCIIs (Type II reaction centers) in the dark (Fv/Fm) and the effective photochemical efficiency of RCII in the light (deltaF/Fm') using pulse amplitude modulation (PAM) fluorometry.

Photosynthesis-irradiance (P/I) curves: To test for the effects of pCO2 and temperature on the ability for Symbiodinium to utilize light and perform photosynthesis, net photosynthesis (P^net), determined from changes in O2 concentrations in seawater, was measured under different irradiances using three corals selected randomly from each treatment tank.

Chlorophyll-a concentration and Symbiodinium density: Chlorophyll-a concentration and Symbiodinium density were determined.

Relevant References:

For full details see Methodology, from C. B. Wall, T.-Y. Fan, P. J. Edmunds (2014) Ocean acidification has no effect on thermal bleaching in the coral. Coral Reefs 33:119-13

Data Processing Description

Dataset Processing Description

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0844785	NSF Division of Ocean Sciences

Instruments

Aquarium chiller

Supplied Description:

Aquatek, Aquasystems, Taiwan

Instrument Type

Generic Name: Aquarium chiller

Generic Description:

Immersible or in-line liquid cooling device, usually with temperature control.

Automatic titrator

Supplied Description:

Open-cell autotitrator (Model DL50, Mettler-Toledo, USA) filled with certified acid titrant (from A. Dickson, Scripps Institution of Oceanography) and equipped with a DG115-SC pH probe (Mettler-Toledo).

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

Conductivity Meter

Supplied Description:

YSI 3100 Conductivity Meter, YSI Inc., USA

Instrument Type

Generic Name: Conductivity Meter

Generic Description:

Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.

Fluorometer

Supplied Description:

Diving-PAM (Waltz, GmbH, Effeltrich, Germany)

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Gas Analyzer

Supplied Description:

Infrared (IR) gas analyzer (S151, Qubit Systems), calibrated against certified reference gas (1,793 ppm CO2, San Ying Gas Co., Taiwan).

Instrument Type

Generic Name: Gas Analyzer

Generic Description:

Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.

Homogenizer

Supplied Description:

Polytron PT2100, Kinematica, USA

Instrument Type

Generic Name: Homogenizer

Generic Description:

A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.

Immersion heater

Supplied Description:

300 watts, Taikong Corp.

Instrument Type

Generic Name: Immersion heater

Generic Description:

Submersible heating element for water tanks and aquaria.

LI-COR LI-193 PAR Sensor

Supplied Name: LI-COR LI-193 PAR

Supplied Description:

Instrument Type

Generic Name: LI-COR LI-193 PAR Sensor

Acronym: LI-COR LI-193 PAR

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0458/

Generic Description:

The LI-193 Underwater Spherical Quantum Sensor uses a Silicon Photodiode and glass filters encased in a waterproof housing to measure PAR (in the 400 to 700 nm waveband) in aquatic environments. Typical output is in micromol s-1 m-2. The LI-193 Sensor gives an added dimension to underwater PAR measurements as it measures photon flux from all directions. This measurement is referred to as Photosynthetic Photon Flux Fluence Rate (PPFFR) or Quantum Scalar Irradiance. This is important, for example, when studying phytoplankton, which utilize radiation from all directions for photosynthesis. LI-COR began producing Spherical Quantum Sensors in 1979; serial numbers for the LI-193 begin with SPQA-XXXXX (licor.com).

Mass Flow Controller

Supplied Name: MFC

Supplied Description:

A350 Gas Concentration Controller (Qubit) solenoid-controlled

Instrument Type

Generic Name: Mass Flow Controller

Acronym: MFC

Generic Description:

Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases

Pump

Supplied Name: pump

Supplied Description:

To mix water in tanks

Instrument Type

Generic Name: Pump

Generic Description:

A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps

Water Temperature Sensor

Supplied Name: Water Temp Sensor

Supplied Description:

1. Microsensor-based temperature regulators (AquaController, Neptune Systems, USA)

2. Certified digital thermometer (Fisher Scientific 15-077-8, +/- 0.05 C)

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
lab	site of experiments	unitless	laboratory
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
CO2_trt	CO2 treatments are ambient vs. elevated (45 vs 85 Pa pCO2)	Pascals	pCO2
temp_trt	Temp treatments are ambient vs. elevated (27.7 vs 30.5 C)	degrees Celsius	temp
tank	Tanks are replicate treatments (n=2 per treatment)	unitless	tank
replicate	The replicate coral within each tank (n= 7)	unitless	replicate
surface_area	surface area of coral	cm^2	no_bcodmo_term
Symb_density	Symbiodinium cell density	total cells	count
Symb_surf_area	Symbiodinium cell surface area	cells/cm2	cell_concentration
chl_a	chlorophyll-a	micrograms	chl_a
chl_a_surf_area	chlorophyll-a per surface area	ug/cm2	chl_a
chl_a_cell	chlorophyll-a per cell	pg/cell	chl_a
alpha	light limited photosynthesis (slope of the curve)	(umolO2 cm-2 h-1)/(umol photons m-2 s-1)	alpha
Pmax_660	net photosynthesis at 660 nm	umol O2 cm-2 h-1	no_bcodmo_term
beta	photoinhibition exponent	(umolO2 cm-2 h-1)/(umol photons m-2 s-1)	beta
respiration	dark aerobic respiraton rates; negative by convention for O2 consumption	umol O2 cm^2/hr	dark_O2_respir
F_prime	fluorescence yield in light (at time t) on day 14	unitless; scale 0 to 1	no_bcodmo_term
Fm_prime	maximum fluorescence yield in light on day 14	unitless; scale 0 to 1	no_bcodmo_term
delta_F_Fm	effective quantum yield on day 14; effective photochemical efficiency of RCII in the light; ?F/Fm' = (Fm' - F')/Fm'	unitless; scale 0 to 1	no_bcodmo_term
Fo	minimum fluorescence yield on day 14	unitless; scale 0 to 1	no_bcodmo_term
Fm	maximum fluorescence yield on day 14	unitless; scale 0 to 1	no_bcodmo_term
Fv_Fm	dark adapted yield on day 14; a measure of photochemical quenching; Fv/Fm= (Fm - Fo)/Fm	unitless; scale 0 to 1	no_bcodmo_term

Database

Contribute Data

Dataset: Symbiodinium_Seriatopora photosynthesis
Deployment: lab_Edmunds_NMMBA

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Symbiodinium_Seriatopora photosynthesisDeployment: lab_Edmunds_NMMBA

Dataset acquisition description

Dataset Processing Description

Dataset: Symbiodinium_Seriatopora photosynthesis
Deployment: lab_Edmunds_NMMBA