Dataset: FL Stegastes Partitus Otolith Summary Data
Deployment: Sponaugle_SCUBA_dives

Summary of otolith microstructure data from Florida Keys Stegastes partitus, 2003-08.

Principal Investigator:

Su Sponaugle (University of Miami Rosenstiel School of Marine and Atmospheric Science, UM-RSMAS)

Contact:

Tauna Rankin (National Marine Fisheries Service, NMFS)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Linkages Between Larvae and Recruitment of Coral Reef Fishes Along the Florida Keys Shelf: an Integrated Field and Modeling Analysis of Population Connectivity in a Complex System (FK Population Connectivity)

Scope and Consequences of Variability in the Early Life History Traits of a Caribbean Coral Reef Fish (FK Fish Recruitment)

Current State:

Final no updates expected

Version:

15 Aug 2014

Version Date:

2014-08-15

Data URL:

https://www.bco-dmo.org/dataset-deployment/526672/data

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Description

Summary of data from analyses of otoliths from Stegastes partitus collected from the Florida Keys.

Methods & Sampling

Dataset acquisition description

Collections were made over many small boat SCUBA dives from 2003-2008; cruises and dives were not numbered.

The otoliths were extracted using standard dissecting techniques and placed in a drop of medium viscosity immersion oil on a microscope slide to clear for a minimum of 30 d. Based on ease of reading, only the lapilli were examined. The clearest lapillus was chosen from each individual and viewed under 400 magnification through a Leica DMLB microscope equipped with a polarized filter between the first stage and light source. The image was captured by a Dage MTI video camera and analyzed using Image Pro Plus 4.5 software (Media Cybernetics). Each otolith was read once blind (i.e., without sample information available) and saved as a digital file. Every 5th individual was measured a second time. The images of the remaining 1029 fish were examined to determine if there was any ambiguity in the placement of the increments and an additional 161 fish were aged a second time, resulting in a total of 418 fish aged twice. Otoliths were rejected where the difference between the first and second reads was > 5%, resulting in six exclusions. Otolith analysis was utilized to determine the following ELHTs: post-settlement age (number of concentric increments after the settlement mark), pelagic larval duration (PLD; number of concentric increments from the primordium to the settlement mark), larval and juvenile growth rates (widths between consecutive increments), and size-at-age (otolith radius-at-each age, including settlement).

Data Processing Description

Dataset Processing Description

Raw data were obtained directly from image processing software. This dataset includes the raw data that have not been manipulated. Please refer to the publications below for details on how these raw data were used.

See two publications using these data:
Rankin TL, & Sponaugle S. 2011. Temperature influences selective mortality during the early life stages of a coral reef fish. PLOS ONE 6: e16814. DOI: 10.1371/journal.pone.0016814
Rankin TL, & Sponaugle S. (in review). Characteristics of settling coral reef fish are related to recruitment timing and success.

BCO-DMO Processing Notes:
- Modified format of dates;
- Modified parameter names;
- Replaced blanks with nd;
- Added 'site_name' field;
- Added 'site_lat' and 'site_lon' from coordinates provided on project metadata form.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0550732	NSF Division of Ocean Sciences
OCE-9986359	NSF Division of Ocean Sciences

Instruments

Fluorescence Microscope

Supplied Name: Leica DMLB microscope

Supplied Description:

The clearest lapillus was chosen from each individual and viewed under 400 magnification through a Leica DMLB microscope equipped with a polarized filter between the first stage and light source. The image was captured by a Dage MTI video camera and analyzed using Image Pro Plus 4.5 software (Media Cybernetics).

Instrument Type

Generic Name: Fluorescence Microscope

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
stage	Life stage of fish upon collection. Larva = late stage larva caught in light trap; Juvenile = < 1 month old juvenile collected during visual survey.	text	stage
date_collected	Year-month-day of collection written as YYYYMMDD.	unitless	date
collection	For larvae this refers to light trap number fish was collected in. For juveniles this refers to habitat of survey and collection: 1 = reef habitat; 2 = rubble habitat.	integer	no_bcodmo_term
site_name	Name of the site.	text	site
site_code	Code that refers to the site of the survey and collection located off of the upper Florida Keys. FR = French Reef; MO = Molasses Reef; PI = Pickles Reef; SI = Sand Island Reef; TR = Triangles; WB = White Banks.	text	Site_ID
site_lat	Latitude of the site in degrees north.	decimal degrees	lat
site_lon	Longitude of the site in degrees west.	decimal degrees	lon
sample	The sample number of the individual fish in the collection.	integer	sample
std_length	Standard length.	millimeters (mm)	fish_len
monthly_cohort	Month and two digit year of collection.	mon-yr	no_bcodmo_term
site_descrip	Description of location of survey and collection sites; offshore = reefs that part of the bank reef tract; inshore = reefs that are inshore patch reefs; only applies to juveniles.	text	site_descrip
collect_catch_or_density	For larvae this refers to total light trap catch; for juveniles this refers to collection density estimated from 15 5 x 1 m transects and then converted to number of juvenile fish per meter-squared.	numeric	no_bcodmo_term
pel_larv_dur	Pelagic larval duration in days which was estimated from the number of daily larval otolith increments.	number of days	no_bcodmo_term
juv_age	Juvenile age in days, which was estimated from the number of daily juvenile otolith increments.	days	age
date_hatched	Date of hatching which was estimated by subtracting PLD and Juv Age from Collect Date. in the format YYYYMMDD	unitless	date
date_spawn	Date of spawning which was estimated by subtracting 4 days from Hatch Date. in the format YYYYMMDD	unitless	date
lunar_day_spawn	Lunar day of spawning which was assigned based on Spawn Date with day 1 corresponding to the new moon; day 8 corresponding to the 1st quarter; day 15 corresponding to the full moon; and day 23 corresponding to the 3rd quarter	integer	no_bcodmo_term
lunar_phase_spawn	Lunar phase of spawning which was assigned based on Lunar Day with 1st quarter moon: days 5-11; 2nd quarter/full moon: days 12-18; 3rd quarter moon: days 19-26; 4th quarter/new moon: days 27-4.	ordinal	no_bcodmo_term
date_settled	Date of settlement which was estimated by subtracting Juv Age from Collect Date. in the format YYYYMMDD	unitless	date
lunar_day_settled	Lunar day of settlement which was assigned based on Settle Date with day 1 corresponding to the new moon; day 8 corresponding to the 1st quarter; day 15 corresponding to the full moon; and day 23 corresponding to the 3rd quarter.	integer	no_bcodmo_term
lunar_phase_settled	Lunar phase of settlement which was assigned based on Lunar Day with 1st quarter moon: days 5-11; 2nd quarter/full moon: days 12-18; 3rd quarter moon: days 19-26; 4th quarter/new moon: days 27-4.	ordinal	no_bcodmo_term
tidal_day_settled	Tidal amplitude day of settlement which was assigned based Settle Date with day 1 corresponding to the maximum tidal amplitude closest to the new moon.	?	no_bcodmo_term
larval_radius	Otolith radius in um at the last daily larval otolith increment.	micrometers (um)	no_bcodmo_term
settle_radius	Otolith radius in um at the settlement otolith increment.	micrometers (um)	no_bcodmo_term
otolith_radius	Otolith radius in um to the edge of the otolith.	micrometers (um)	no_bcodmo_term
larval_growth_avg	Mean daily larval otolith increment width in um.	micrometers (um)	growth
juv_growth_avg	Mean daily juvenile otolith increment width in um.	micrometers (um)	growth
juv_growth_1_2_d_avg	Mean daily juvenile otolith increment width over the first 2 days post-settlement in um.	micrometers (um)	growth
temp_larval	Mean water temperature at 21 m depth at Conch Reef in the upper Florida Keys averaged over the larval period of each Monthly Cohort.	degrees Celsius (?)	no_bcodmo_term
temp_juv	Mean water temperature at 21 m depth at Conch Reef in the upper Florida Keys averaged over the juvenile period of each Monthly Cohort.	degrees Celsius (?)	no_bcodmo_term
density_juv	Density of juvenile conspecifics (20-30 mm SL) estimated from 15 5 x 1 m transects and then converted to number of fish per meter-squared; only surveyed in 2007 and 2008.	number of fish per square meter (fish/m^2)	no_bcodmo_term
density_int	Density of intermediate conspecifics (30-50 mm SL) estimated from 15 5 x 1 m transects and then converted to number of fish per meter-squared; only surveyed in 2007 and 2008.	number of fish per square meter (fish/m^2)	no_bcodmo_term
density_adult	Density of adult conspecifics ( > 50 mm SL) estimated from 15 5 x 1 m transects and then converted to number of fish per meter-squared; only surveyed in 2007 and 2008.	number of fish per square meter (fish/m^2)	no_bcodmo_term

Database

Contribute Data

Dataset: FL Stegastes Partitus Otolith Summary Data
Deployment: Sponaugle_SCUBA_dives

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: FL Stegastes Partitus Otolith Summary DataDeployment: Sponaugle_SCUBA_dives

Dataset acquisition description

Dataset Processing Description

Dataset: FL Stegastes Partitus Otolith Summary Data
Deployment: Sponaugle_SCUBA_dives