» submit.bco-dmo.org » How-to Guide » FAQs
BCO-DMO Blog Find out what's happening at BCO-DMO.
Protein content was determined spectrophotometrically using the Bio-Rad Coomassie Blue assay in the microtiter plate protocol (BioRad Life Sciences Research, CA). At each sampling, 1 group of 8 larvae from each tub within each tank (i.e., 2 replicates tank-1) was frozen in liquid nitrogen and stored. Proteins were solubilized in 0.1 M NaOH, aided by sonication (10% amplitude for 15 s using a Branson Digital Sonifier S-250D, USA) and warming at 50 °C for 5 h. The extract was neutralized with 1 M HCl and processed in triplicate with the addition of the dye reagent. Following 30 min incubation, absorbances were measured at 595 nm using a plate reading spectrophotometer (Biotek Synergy H4 Hybrid Reader, USA), and converted to protein using a calibration prepared from bovine serum albumin. Protein content was expressed as microgram larva-1.
The 'ambient' and 'high' pCO2 levels: 49.4 Pa versus 86.2 Pa The 'ambient' and 'high' temperatures: 24.00 °C [ambient] versus 30.49 °C
Data also available from PANGAEA: doi:10.1594/PANGAEA.823582
BCO-DMO processing notes: - added conventional header with dataset name, PI name, version date, reference information - renamed parameters to BCO-DMO standard - added lab, lat, lon columns - reduced number of significant digits
Aquatech Ac11 or Shyeh Duwai Enterprise
Immersible or in-line liquid cooling device, usually with temperature control.
Infra Red gas analyzer (S151, Qubit Systems)
Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.
A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
300 Wheaters, Taikong Corporation
Submersible heating element for water tanks and aquaria.
cosine-corrected quantum light meter (Li-Cor LI-192 attached to an LI-1400)
The LI-192 Underwater Quantum Sensor (UWQ) measures underwater or atmospheric Photon Flux Density (PPFD) (Photosynthetically Available Radiation from 360 degrees) using a Silicon Photodiode and glass filters encased in a waterproof housing. The LI-192 is cosine corrected and features corrosion resistant, rugged construction for use in freshwater or saltwater and pressures up to 800 psi (5500 kPa, 560 meters depth). Typical output is in um s-1 m-2. The LI-192 uses computer-tailored filter glass to achieve the desired quantum response. Calibration is traceable to NIST. The LI-192 serial numbers begin with UWQ-XXXXX. LI-COR has been producing Underwater Quantum Sensors since 1973.
These LI-192 sensors are typically listed as LI-192SA to designate the 2-pin connector on the base of the housing and require an Underwater Cable (LI-COR part number 2222UWB) to connect to the pins on the Sensor and connect to a data recording device.
The LI-192 differs from the LI-193 primarily in sensitivity and angular response. 193: Sensitivity: Typically 7 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 3% error over 360° at 90° from normal axis. Angular Response: < ± 4% error up to ± 90° from normal axis. 192: Sensitivity: Typically 4 uA per 1000 umol s-1 m-2 in water. Azimuth: < ± 1% error over 360° at 45° elevation. Cosine Correction: Optimized for underwater and atmospheric use. (www.licor.com)
A Ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running the manufacturers software (OOISensor, version 1.00.08). The optrode was calibrated using a zero solution (0.01 M Na2B4O7·10H2O supersaturated with Na2SO3) and 100% air saturation using water-saturated air at the treatment temperature.
An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.
BioRad Life Sciences Research, CA
Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
- USB2000, Ocean Optics
- plate reading spectrophotometer (Biotek Synergy H4 Hybrid Reader, USA)
An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.
Branson Digital Sonifier
Instrument that applies sound energy to agitate particles in a sample.
certified digital thermometer (Model 15-077-8, Fisher Scientific,±0.05 °C)
General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).
laboratory
latitude; north is positive
longitude; east is positive
sample identification
target temperature
pCO2 treatment: ambient (47.5 - 49.3 Pa) or high (85.2 - 87.2 Pa)
days since start of experiment
concentration of protein in ?
number of larvae
mean of ?
standard deviation of ?
?
actual concentration of protein in ?
sample volume?
total protein in 8 larvae
number of larvae in sample
protein content per larva in milligrams
protein content per larva in micrograms