Dataset: Hood Canal - 10 uM salicylaldoxime ligand
Deployment: CB985

Copper data with 10 uM SA lingand from Hood Canal, Puget Sound, Washington, USA

View Data: For data, See Dataset Metadata Page: https://www.bco-dmo.org/dataset/540987

Principal Investigator:

James W. Moffett (University of Southern California, USC)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Significance of nitrification in shaping planktonic biodiversity in the ocean (Nitrification and Marine Planktonic Biodiversity)

Current State:

Preliminary and in progress

Version:

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Description

Water samples were collected at various depths from the Hood Canal and the Cu2 + concentrations, organic ligand (L) concentrations and their corresponding conditional stability constants (K) were characterized using a competitive ligand exchange adsorptive cathodic stripping voltammetry (CLE-ACSV) adapted from Jacquot et al. (2013) and Moffett and Dupont (2007) with salicylaldoxime (SA) as the competing ligand.

Methods & Sampling

Dataset acquisition description

All samples for total dissolved copper on this cruise were well above our detection limit of 100pM determined from the standard deviation of procedural blanks using our ICP-MS methods.

Cu speciation was determined using cathodic stripping voltammetry on samples titrated with added copper.

Data Processing Description

Dataset Processing Description

Data points below the detection limit of the cathodic stripping voltammetry technique (~ 0.15 nM) were not used in the titration. However, all titrations had a significant number of points to fit a non-linear regression curve. In instances where the fit did not converged, or converged at a non-realistic value (e.g. negative numbers) the data were flagged.

The standard error for [Cu2+] is asymmetric because it is not determined the same way - hence the different values.

The Cu2+ error propagation was derived from the standard deviations of K and [L] when used in equation 11 from Moffett and Dupont 2007 to calculate [Cu2+].

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1046098	NSF Division of Ocean Sciences

Instruments

BASi Controlled Growth Mercury Electrode

Supplied Name: BASI CGME

Supplied Description:

BioAnalytical Systems (BASi) Controlled Growth Mercury Electrode set to the Static Mercury Drop setting (drop size: 14) and interfaced with a BASi Epsilon ε2 voltammetric analyzer.

Instrument Type

Generic Name: BASi Controlled Growth Mercury Electrode

Acronym: BASI CGME

Generic Description:

Bioanalytical Systems (BASi) Mercury drop electrodes are generated by the BASi Controlled Growth Mercury Electrode (CGME) in three modes:

DME (Dropping Mercury Electrode) - mercury is allowed to flow freely from the reservoir down the capillary and so the growth of the mercury drop and its lifetime is controlled by gravity. (The optional 100 um capillary is recommended for this mode.)

SMDE (Static Mercury Drop Electrode) - the drop size is determined by the length of time for which the fast-response capillary valve is opened, and the drop is dislodged by a drop knocker. The dispense/knock timing is microprocessor-controlled and is typically coordinated with the potential pulse or square-wave waveform. This mode can also used to generate the Hanging Mercury Drop Electrode required for stripping experiments.

CGME (Controlled Growth Mercury Electrode) - the mercury drop is grown by a series of pulses that open the capillary valve. The number of pulses, their duration, and their frequency can be varied by PC control, providing great flexibility in both the drop size and its rate of growth. This CGME mode can be used for both polarographic and stripping experiments.

http://www.basinc.com/products/ec/cgme.php

CTD Sea-Bird SBE 911plus

Supplied Name: CTD SBE 911plus

Supplied Description:

Instrument Type

Generic Name: CTD Sea-Bird SBE 911plus

Acronym: CTD SBE 911plus

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/

Generic Description:

The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics

GO-FLO Bottle

Supplied Name: GO-FLO

Supplied Description:

10 L Teflon-coated Go-Flo bottles (General Oceanics) attached to Kevlar wire

Instrument Type

Generic Name: GO-FLO Bottle

Acronym: GO-FLO

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/30/

Generic Description:

GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths.

Inductively Coupled Plasma Mass Spectrometer

Supplied Name: ICP Mass Spec

Supplied Description:

Finnegan Element 2 (Thermo Scientific)

Instrument Type

Generic Name: Inductively Coupled Plasma Mass Spectrometer

Acronym: ICP Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/

Generic Description:

An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer.

Voltammetry Analyzers

Supplied Description:

BASi Epsilon ε2 voltammetric analyzer

Instrument Type

Generic Name: Voltammetry Analyzers

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB35/

Generic Description:

Instruments that obtain information about an analyte by applying a potential and measuring the current produced in the analyte.

Parameters

None available yet

Database

Contribute Data

Dataset: Hood Canal - 10 uM salicylaldoxime ligand
Deployment: CB985

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Hood Canal - 10 uM salicylaldoxime ligandDeployment: CB985

Dataset acquisition description

Dataset Processing Description

Dataset: Hood Canal - 10 uM salicylaldoxime ligand
Deployment: CB985