Dataset: Terminal restriction fragments from 16S rRNA genes amplified from coral-associated microbes in response to temperature stress from Kaneohe Bay, Oahu, Hawaii in 2007 (MiCoDe project)

ValidatedFinal no updates expectedVersion 2015-02-20 (2015-02-20)Dataset Type:Unknown

Principal Investigator: Michael S. Rappé (University of Hawaiʻi at Mānoa)

Contact: Jennifer Salerno (George Mason University)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: The Development of Microbial Associations in Major Reef Building Corals of the Pacific Ocean (MiCoDe)

This dataset includes the abundance of terminal restriction fragments from 16S rRNA genes amplified from coral-associated microbes. An array of closed system, precision-controlled experimental aquaria were used to investigate shifts in the bacterial communities associated with the Hawaiian reef building coral Porites compressa in coral fragments exposed to an abrupt 1◦C increase in seawater temperature above ambient summer levels following a 10 d acclimation at ambient temperature. Terminal restriction fragment length polymorphism analysis (T-RFLP) of bacterial small subunit ribosomal RNA (SSU rRNA) genes was conducted.

Related Reference:

Salerno, J. L., Reineman, D. R., Gates, R. D., and Rappé, M. S. (2011) The effect of a sublethal temperature elevation on the structure of bacterial communities associated with the coral Porites compressa. Journal of Marine Biology 2011. doi:10.1155/2011/969173


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