Dataset: Long-term PaV1 survey
Deployment: Butler_FloridaBay

Long-term PaV1 lobster surveys Florida bayside, 1999-2014

Principal Investigator:

Mark Butler (Old Dominion University, ODU)

Co-Principal Investigator:

Donald Behringer (University of Florida, UF)

Claire B. Paris-Limouzy (University of Miami Rosenstiel School of Marine and Atmospheric Science, UM-RSMAS)

Jeffery Shields (Virginia Institute of Marine Science, VIMS)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Connectivity of disease in marine Ecosystems: multi-scale dynamics of a viral disease infecting caribbean spiny lobster (Lobster disease connectivity)

Current State:

Final no updates expected

Version:

2015-04-20

Version Date:

2015-04-20

Data URL:

https://www.bco-dmo.org/dataset-deployment/556170/data

Expand/Collapse All

Description

From 1999-2014, lobsters were briefly captured in Florida Bay. Measurements taken included sex, length, molt condition, evidence of injury and disease. PaV1 virus PCR results were logged.

Related References:

Portions of these data have been reported in the following publication:

Behringer, D.C., M.J. Butler, J. Shields, and J. Moss. 2011. A review of Panulirus argus Virus 1 – a Decade after its Discovery. Diseases of Aquatic Organisms 94: 153–160.

Methods & Sampling

Dataset acquisition description

At each permanent 25m x 25m sample location (hard-bottom habitat 1.5 – 3.0 m depth), delineated by rope border affixed to seafloor, two divers search the entire site for spiny lobsters (Panulirus argus). All lobsters sighted are collected in hand nets by divers and brought aboard the topside vessel where the data are collected & recorded. Lobsters are then returned live to the original site. Hemolymph samples are stored in labeled vials with 90% ETOH and kept in dark and on ice until return to shore where they are then stored in a -40C freezer until processed.

Data Processing Description

Dataset Processing Description

BCO-DMO Processing:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard
- added lat/lon
- changed NA and ? to nd
- replace blanks with 0 for injuries, disease, blood sample, PCR Results; replaced other blanks with nd

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0928930	NSF Division of Ocean Sciences
OCE-0929086	NSF Division of Ocean Sciences

Instruments

Thermal Cycler

Supplied Name: pCR

Supplied Description:

Instrument Type

Generic Name: Thermal Cycler

Generic Description:

A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.

(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
year	year of survey	yyyy	year
date	date of survey	yyyy-mm-dd	date
site	survey site	unitless	site
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
sex	sex of lobster	unitless	sex
length_carap	carapace length of tethered lobster	millimeters	length
molt_cond	visual assessment of molt condition: I = intermolt; PR = premolt; PO = postmolt	unitless	no_bcodmo_term
leg_old_new	old and new injuries number of old/new leg injuries if any	unitless	no_bcodmo_term
ant_old_new	number of old/new antennae injuries if any	unitless	no_bcodmo_term
other_old_new	number of old/new other injuries if any	unitless	no_bcodmo_term
visible_disease_flag	signs of PaV1 infection in lobster obvious to naked eye; 0 = no; 1 = yes	unitless	no_bcodmo_term
blood_sample	hemolymph sample vial label if any	unitless	no_bcodmo_term
PCR_result	positive or negative PCR test for presence of PaV1 virus DNAa	unitless	no_bcodmo_term
comments	comments	unitless	comment

Database

Contribute Data

Dataset: Long-term PaV1 survey
Deployment: Butler_FloridaBay

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Long-term PaV1 surveyDeployment: Butler_FloridaBay

Dataset acquisition description

Dataset Processing Description

Dataset: Long-term PaV1 survey
Deployment: Butler_FloridaBay