Prochlorococcus MED4 was acclimated to low (150 ppm) or high (1000 ppm) CO2. The cultures were then concentrated and placed in the chamber of a membrane inlet mass spectrometer (MIMS; Pfieffer QMS220). Dissolved inorganic carbon (DIC) was gradually added to the culture and inorganic carbon fluxes were measured using MIMS as described in Hopkinson et al. 2014.
Relevant Reference:
Hopkinson, B.M., J.N. Young, A. L. Tansik, and B. J. Binder. 2014. The minimal CO2-concentrating mechanism of Prochlorococcus spp. MED4 is effective and efficient. Plant Physiology 166: 2205-2217.
Hopkinson, B. M. (2015) Experimental results on inorganic carbon fluxes of Prochlorococcus MED4 cultured under high and low CO2 concentrations (OA phytoplankton physiology project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-07-29) Version Date 2015-07-29 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/563152 [access date]
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