Dataset: Candidatus Thioglobus autotrophica strain EF1
Deployment: TN268

De novo assembly of the T. autotrophica EF1 genome was conducted using the Hierarchical Genome Assembly Process (HGAP) (Koren et al, 2012). Briefly, single reads were mapped to seed reads, a Celera assembler created overlapping consensus sequences, and the remaining inDel and base substitution errors were removed. This method has been found to produce highly accurate, complete de novo assemblies for small prokaryotic genomes (Roberts et al, 2013). HGAP assembly of the T. autotrophica EF1 genome resulted in a single contiguous sequence that was closed using a single PCR reaction. The complete genome sequence of T. autotrophica EF1 used 100% of cleaned reads with an average coverage of 106x, indicating high confidence in a single circular chromosome 1,512,449 bp in length. Protein coding sequences were identified and annotated via NCBI’s automatic Prokaryotic Genome Annotation Pipeline and were checked against RAST annotations, IMG annotations, and in some cases by phylogenetic analyses. Discrepancies were corrected and final annotations were submitted to NCBI.