Dataset: limpet mass and body volume
Deployment: Denny_2013

Limpet mass and body volume data for respiration measurements

Principal Investigator:

Dr Mark W Denny (Stanford University - Hopkins, Stanford-HMS)

Co-Principal Investigator:

Dr Bengt J Allen (California State University Long Beach, CSULB)

Contact:

Luke P. Miller (Stanford University - Hopkins, Stanford-HMS)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Environmental Variability, Functional Redundancy, and the Maintenance of Ecological Processes: Experiments in a Model Ecosystem (Experiments in a Model Ecosystem)

Current State:

Final no updates expected

Version:

2016-01-13

Version Date:

2016-01-13

Data URL:

https://www.bco-dmo.org/dataset-deployment/630068/data

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Description

Growth data for animals on experimental plates in the field during 2013 was collected monthly via digital photographs and measured using ImageJ software. Respiration of limpets collected from the field during summer 2013 was measured in air or seawater at a range of temperatures for one hour or two hours.

Related Reference:

Miller, L.P., B.J. Allen, F.A. King, D.R. Chilin, V.M. Reynoso and M.W. Denny (2015). Warm microhabitats drive both increased respiration and growth rates of intertidal consumers. Marine Ecology Progress Series 522: 127-143 doi: http://dx.doi.org/10.3354/meps11117

Download R code: 2013_limpet_mass_analysis.R

Related Datasets (includes metadata)	Download original data files
limpet mass and body volume	2013_limpet_mass_master.csv
limpet aquatic respiration	2013_summer_aquatic_respiration_rates.csv
limpet aerial respiration	2013_summer_aerial_respiration_rates.csv

These data are also available at the Stanford Digital Repository: https://purl.stanford.edu/mz343tz6255

Methods & Sampling

Dataset acquisition description

Oxygen measurements taken using Ocean Optics FOXY fluorescence-based optode. Limpet mass data for all measured animals is included. Detailed methodology is available in Miller et al (2015).

Data Processing Description

Dataset Processing Description

Growth measurements were made by analyzing limpet shell projected area in ImageJ. Limpet respiration time series were used to estimate oxygen consumption rate. Complete analysis for size measurements and derived respiration rates are provided in the attached R code.

BCO-DMO Processing:

- added conventional header with dataset name, PI name, version date
- renamed parameters to BCO-DMO standard
- replaced NA with nd (no data)
- replaced spaces with underscores
- sorted data by air_water, then species, then temp

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1130095	NSF Division of Ocean Sciences
OCE-1131038	NSF Division of Ocean Sciences

Instruments

Water Temperature Sensor

Supplied Name:

Supplied Description:

iButton temperature datalogger (DS1921G, Maxim Integrated)

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
air_water	whether respiration trial was run in air or water (Reminder that many limpets in this data file were run as pilot trials and not included in the published analysis.)	unitless	no_bcodmo_term
species	Lottia species name	unitless	species
temp	temperature of respiration trial	degrees Celsius	temp
limpet	unique identifying label for each limpet consisting of a 3-character species name abbreviation and a 3 or 4 digit number: lim = Lottia limatula; dig = L. austrodigitalis; sca = L. scabra; pel = L. pelta	unitless	sample
mass_submerged_g	mass of the submerged live limpet in seawater; used for calculation of volume	grams	weight
mass_air_g	wet mass of live limpet (shell + tissue)	grams	weight
mass_empty_shell_g	dry mass of empty limpet shell	grams	weight
mass_tin_tare_g	mass of tin foil weighing dish; used for drying tissue	grams	weight
mass_dry_gross_g	mass of tin foil dish and dried tissue	grams	weight
image_id	corresponding image name for limpet shell (used to calculate projected area of shell)	unitless	sample
mass_tissue_live_g	wet tissue mass; calculated as difference between mass_air_g and mass_empty_shell_g	grams	weight
mass_net_dry_tissue_g	dried tissue mass; calculated as difference between mass_dry_gross_g and mass_tin_tare_g	grams	weight
mass_net_disp_g	net displaced mass; calculated as the difference betweenmass_air_g and mass_submerged_g	grams	weight
vol_limpet_cm3	limpet displaced volume; calculated as mass_net_disp_g * 1.0247 (density of local seawater at 15C = 1.0247 g/cm^3).	cm^3	disp_vol
area_shell_mm2	projected area of limpet shell when viewed from above; measured using the digital image of the shell in ImageJ by painting the outline of the shell and calculating the enclosed area. This same method was used to estimate limpet growth in the field using digital images taken from overhead.	mm^2	no_bcodmo_term
date_collection	date that limpet was collected from the field	mm/dd/yyyy	date
date_trial	date of respiration measurements	mm/dd/yyyy	date
comment	lists whether limpet shell was intact or chipped. Chipped shells should not be used to calculate the relationship between limpet mass and projected area measured in overhead images.	unitless	comment

Database

Contribute Data

Dataset: limpet mass and body volume
Deployment: Denny_2013

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: limpet mass and body volumeDeployment: Denny_2013

Dataset acquisition description

Dataset Processing Description

Dataset: limpet mass and body volume
Deployment: Denny_2013