Dataset: lab naupliar grazing: prey densities
Deployment: Goetze_2012-2013

All samples were collected from surface water in Southern Kaneohe Bay, Oahu, Hawaii (21°25’56.7”N, 157°46’47.1”W).

Pro, Syn, Peuk, PE+Peuk, HBact data: These data were collected and analyzed by flow cytometry as described in Selph et al. (2011).  Briefly, samples were preserved (0.05% paraformaldehyde, final concentration), and frozen to -80°C within 1 h.  Batches of samples were thawed, stained with Hoechst 34442 (1 µg/mL final concentration) and analyzed on a flow cytometer (Beckman Coulter Altra) with dual laser excitation (UV range and 488 nm, 200 mW and 1 W, respectively), mated to a Harvard Apparatus syringe pump for quantitative delivery (100 µl at 50 µl min-1).  Resulting listmode files were analyzed off-line using FlowJo software (Treestar).  Populations determined, based on red fluorescence (chlorophyll, 680±20 nm), orange fluorescence (phycoerythrin, 575±20 nm), blue fluorescence (DNA, 450±40 nm), and light scatter signals (forward and 90°).  Distinguishable populations were Prochlorococcus, Synechococcus, Photosynthetic Eukaryotes (PEUK), High phycoerythrin-containing PEUK (PE+Peuk), and non-pigmented prokaryotes (a.k.a. heterotrophic bacteria, HBACT).