Dataset: PAM fluorescence
Deployment: Guam_Reef_Surveys_2014

Underwater fluorescence, Guam reefs 2014

Principal Investigator:

Dr Kiho Kim (American University, AU)

Co-Principal Investigator:

Dr David M Baker (University of Hong Kong, HKU-SBS)

Dr Laurie J Raymundo (University of Guam Marine Laboratory, UOGML)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Documenting bleaching susceptibility and resilience in Guam, Micronesia (Reef Resilience in Guam)

Current State:

Preliminary and in progress

Version Date:

2016-06-17

Data URL:

https://www.bco-dmo.org/dataset-deployment/639992/data

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Description

Methods & Sampling

Dataset acquisition description

Maximum excitation pressure of Symbiodinium assemblages was assessed as an indicator for holobiont photosynthetic performance of Pocillopora damicornis in shallow (1-2m), back reef and P. eydouxi in deeper fore-reef (>3m). Effective quantum yield (F/Fm’) was measured using a Walz Diving Pam Fluorometer (Germany) via saturation pulse fluorometry at 1cm away from branch tips during midday. Maximum quantum yield (Fv/Fm) was measured under custom shading devices which were designed to dark-acclimate corals in situ for 30 minutes. Maximum excitation pressure (Q) was calculated using the formula, Q = 1-(F/Fm’)/(Fv/Fm).

Data Processing Description

Dataset Processing Description

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date
- renamed parameters to BCO-DMO standard
- replaced spaces with underscores
- added site_code, zone, lat and lon columns
- reformatted date from d-Mon-yy to yyyy-mm-dd
- replaced blank cells with 'nd' (no data)

Version 2 (2021-01-28) replaced version 1 (2016-06-17) - revised site lat/lon

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1418673	NSF Division of Ocean Sciences

Instruments

Fluorometer

Supplied Name:

Supplied Description:

Diving PAM Underwater Fluorometer (Walz), an instrument for studying in situ photosynthesis of underwater plants.

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
date	survey date	yyyy-mm-dd	date
time_local	local time	HH:MM:SS	time_local
data_recorder	initials of person recording data: Code Data = Recorder; Home Institution DMB = David Baker;U Hong Kong DRB = David Burdick; U of Guam / NOAA JC = Jeried Calor; U of Guam KK = Kiho Kim; American University LRJ = Laurie Raymundo; U of Guam ND = Nicolas Duprey; U of Hong Kong PT = Phil Thompson; U of Hong Kong SV = Susie Vulpas; American University	unitless	person
colony_id	individual colony identification number	unitless	sample
site	survey location near Guam Island	unitless	site
site_code	site code	unitless	site
zone	part of reef surveyed	unitless	site_descrip
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
taxon_code	taxonomic code of corals. See related dataset https://www.bco-dmo.org/dataset/639865	unitless	taxon_code
light_dark	whether measurement was taken with light (L) or dark (D) adapted corals	unitless	no_bcodmo_term
F	chlorophyll florescence (excess energy from a photon re-emitted as light) while reactions centers of photosystem II are at a steady state	unitless	no_bcodmo_term
Fm_prime	maximum chlorophyll florescence based on whichever light environment the chlorophyll is adapted to	unitless	no_bcodmo_term
PAR	photosynthetic active radiation; measure of light intensity	micromol quanta m-2 s-1	PAR
Y_II	maximum photochemical yield of photosystem II; measure of overall photochemical efficiency	unitless	no_bcodmo_term
ETR	electron transport rate; measure of overall photosynthetic capacity	micromol electrons m-2 s-1	ETR

Database

Contribute Data

Dataset: PAM fluorescence
Deployment: Guam_Reef_Surveys_2014

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: PAM fluorescenceDeployment: Guam_Reef_Surveys_2014

Dataset acquisition description

Dataset Processing Description

Dataset: PAM fluorescence
Deployment: Guam_Reef_Surveys_2014