Dataset: SEEPC larval collections: SyPRID
Deployment: AT29-04

AUV Sentry collections of invertebrate larvae close to the bottom near methane seep sites at the Western Atlantic Margin, 2015 (SEEPC Project)

Principal Investigator:

Craig M. Young (University of Oregon, OIMB)

Co-Principal Investigator:

Svetlana A. Maslakova (University of Oregon, OIMB)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Connectivity in western Atlantic seep populations: Oceanographic and life-history processes underlying genetic structure (SEEPC)

Current State:

Final no updates expected

Deployment Synonyms:

Seep Connectivity, WAM, Western Atlantic Margin

Coordinated Deployments:

AT29-04_Alvin_Dives

Version Date:

2016-09-30

Data URL:

https://www.bco-dmo.org/dataset-deployment/659973/data

Expand/Collapse All

Description

This dataset is a collection log showing the preliminary identification of larval types and the final disposition of individual larval specimens collected with the AUV Sentry.

Related datasets:
SEEPC larval collections: MOCNESS
larval type codes

Methods & Sampling

Dataset acquisition description

AUV Sentry was deployed to perform a programmed survey pattern over the chosen site. Once Sentry was a given distance from the bottom, the SyPRID sampler was turned on to collect larvae until the start of Sentry’s ascent. During descent and ascent, a valve was closed to prevent contamination of the deep water sample. Upon recovery, all samples were sorted completely over the next 12 hours or less, picking all individual invertebrate larvae from the samples, assigning morphotypes, photographing with a microscope, and preserving either for Scanning Electron Microscopy (glutaraldehyde) or for molecular genetics (95% ETOH).

Data Processing Description

Dataset Processing Description

Larvae of some of the major groups (notably molluscs and bryozoans) were sequenced.

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date
- renamed some parameters to BCO-DMO standard
- replaced blanks and '-' with 'nd' (no data)
- reformatted date from m/d/yyyy to yyyy-mm-dd
- removed 'm' from altitude values
- added cruise_id, lat and lon columns
- removed or replaced special characters

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1030453	NSF Division of Ocean Sciences

Instruments

AUV Sentry

Supplied Description:

Fitted out with the SyPRID (Sentry’s Precision Robotic Impeller Driven) Sampler

Instrument Type

Generic Name: AUV Sentry

Generic Description:

The autonomous underwater vehicle (AUV) Sentry is a fully autonomous underwater vehicle capable of exploring the ocean down to 6,000 meters (19,685 feet) depth. Sentry builds on the success of its predecessor the ABE, with improved speed, range, and maneuverability.

Sentry's hydrodynamic shape also allows faster ascents and descents. Sentry carries a superior science sensor suite and an increased science payload enabling it to be used for both mid-water and near-seabed oceanographic investigations. Sentry produces bathymetric, sidescan, subbottom, and magnetic maps of the seafloor and is capable of taking digital bottom photographs in a variety of deep-sea terrains such as mid-ocean ridges, deep-sea vents, and cold seeps at ocean margins. Sentry is uniquely able to operate in extreme terrain, including volcano caldera and scarps. Sentry's navigation system uses a doppler velocity log and inertial navigation system, aided by acoustic navigation systems (USBL or LBL). The USBL system also provides acoustic communications, which can be used to obtain the vehicle state and sensor status as well as to retask the vehicle while on the bottom. In addition its standard sensors, Sentry has carried a variety of science-supplied sensors, including the Nakamura redox potential probe, ACFR 3-D imaging system, and the Tethys in-situ mass spectrometer.

Sentry can be used to locate and quantify hydrothermal fluxes. Sentry is also capable of a much wider range of oceanographic applications due to its superior sensing suite, increased speed and endurance, improved navigation, and acoustic communications. Sentry can be used as a stand alone vehicle or in tandem with Alvin or an ROV to increase the efficiency of deep-submergence investigations.

More information is available from the operator site at URL: http://www.whoi.edu/main/sentry

Sentry Precision Robotic Impeller Driven Sampler

Supplied Name: SyPRID

Supplied Description:

Instrument Type

Generic Name: Sentry Precision Robotic Impeller Driven Sampler

Acronym: SyPRID Sampler

Generic Description:

The SyPRID (Sentry Precision Robotic Impeller Driven) sampler is an innovative deep-rated (6000 m) plankton sampler that partners with the Sentry Autonomous Underwater Vehicle (AUV) to obtain paired, large-volume plankton samples at specified depths and survey lines to within 1.5 m of the seabed and with simultaneous collection of sensor data.

SyPRID uses a perforated Ultra-High-Molecular-Weight (UHMW) plastic tube to support a fine mesh net within an outer carbon composite tube (tube-within-a-tube design), with an axial flow pump located aft of the capture filter. The pump facilitates flow through the system and minimizes the bow wave at the mouth opening. The cod end, a hollow truncated cone, is also made of UHMW plastic and is designed to 'soften' the landing of zooplankton on the capture surface. SyPRID attaches as a saddle-pack to the Sentry vehicle.

Sentry itself is configured with a flight control system that enables autonomous survey paths to altitudes as low as 1.5 m. In its inaugural deployment at the Blake Ridge Seep (2160 m) on the US Atlantic Margin, SyPRID was operated for 6 h at an altitude of 5 m. It recovered plankton samples from that stratum in excellent condition and with greater larval numbers than recovered in a typical 'near-bottom' MOCNESS sample from comparable habitats and depths. The prototype SyPRID and its next generations will enable studies of plankton or other particulate distributions associated with patchy habitats, localized physico-chemical strata (e.g., above and below the thermocline), or discrete water masses at an unprecedented spatial resolution for a large volume system [1].

More information is available by contacting:
Carl Kaiser
Program Manager
Applied Ocean Physics & Engineering
NDSF AUV Operations Manager
Office Phone: +1 508 289 3269
ckaiser@whoi.edu

[1] Billings, A., Kaiser, C., Young, C. M., Hiebert, L. S., Cole, E., Wagner, J. K. S., & Van Dover, C. L. (2017). SyPRID sampler: A large-volume, high-resolution, autonomous, deep-ocean precision plankton sampling system. In Deep Sea Research Part II: Topical Studies in Oceanography (Vol. 137, pp. 297–306). Elsevier BV. https://doi.org/10.1016/j.dsr2.2016.05.007

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_id	cruise identification	unitless	cruise_id
date	date of sampling formatted as yyyy-mm-dd	year-month-day	date
site	sampling site	unitless	site
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
dive	AUV Sentry dive number	unitless	dive_id
filtration_time	SyPRID Sampler filtration time formatted as HH:MM	hours:minutes	duration
altitude	distance sampled above the sea bottom	meters	alt_vehicle
larva_id	unique number assigned to each individual larva	unitless	sample
larval_type	abbreviation for the general larval form	unitless	taxon
morphotype	initial morphological classification; numbered sequentially within larval forms	unitless	taxon
fixation	method of preservation	unitless	no_bcodmo_term

Database

Contribute Data

Dataset: SEEPC larval collections: SyPRID
Deployment: AT29-04

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: SEEPC larval collections: SyPRIDDeployment: AT29-04

Dataset acquisition description

Dataset Processing Description

Dataset: SEEPC larval collections: SyPRID
Deployment: AT29-04