Dataset: Grazing experiment 4a: short term grazing by Gyrodinium
Deployment: Lab_Olson_B

Short term data for low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Gyrodinium grazers

Principal Investigator:

M Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Brooke Love (Western Washington University, WWU)

Suzanne Strom (Western Washington University, WWU)

Student:

Kelly Ann Still (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)

Data URL:

https://www.bco-dmo.org/dataset-deployment/668667/data

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Description

Related Reference:

Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO₂ induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet

Methods & Sampling

Dataset acquisition description

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. On day 9 Rhodomonas cells from the treatments were fed to starved Gyrodinium dominans cells for 15, 45, and 90 minutes. A 24-hour time point was also taken but scored at either “1” for containing any food or “0” for no food in the vacuole. During each sampling time point, 20 ml of experiment volume was removed, fixed with 0.5% glutaraldehyde and stained with DAPI. Samples were stored at 4° C for 12 hours to allow time for the DAPI to stain. This volume was filtered onto a 5 µm pore size polycarbonate filter with, which was then put on a slide with immersion oil and kept frozen until evaluation. Slides were evaluated under 400x using an epi-fluorescent microscope under blue-light excitation. The first 100 microzooplankton on each slide were assessed for each replicate/treatment, and individual prey cells were counted in the grazer food vacuole.

Data Processing Description

Dataset Processing Description

These data are unprocessed counts of the Rhodomonas cells ingested by each Gyrodinium grazer.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- nd (no data) was entered into all blank cells

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0961229	NSF Division of Ocean Sciences

Instruments

Fluorescence Microscope

Supplied Name: epi-fluorescent microscope under blue-light excitation

Supplied Description:

For cell counts

Instrument Type

Generic Name: Fluorescence Microscope

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
grazer_analyzed	sample identifier: individual grazer analyzed	unitless	sample
amb_15min_A	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 15 minute grazing period; replicate A	cells	count
amb_15min_B	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 15 minute grazing period; replicate B	cells	count
amb_15min_C	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 15 minute grazing period; replicate C	cells	count
amb_45min_A	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 45 minute grazing period; replicate A	cells	count
amb_45min_B	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 45 minute grazing period; replicate B	cells	count
amb_45min_C	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 45 minute grazing period; replicate C	cells	count
amb_90min_A	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 90 minute grazing period; replicate A	cells	count
amb_90min_B	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 90 minute grazing period; replicate B	cells	count
amb_90min_C	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 90 minute grazing period; replicate C	cells	count
amb_24hr_A	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 24 hour grazing period; replicate A	cells	count
amb_24hr_B	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 24 hour grazing period; replicate B	cells	count
amb_24hr_C	Rhodomonas cells counted inside the grazer: ambient pCO2 level; 24 hour grazing period; replicate C	cells	count
mod_15min_A	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 15 minute grazing period; replicate A	cells	count
mod_15min_B	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 15 minute grazing period; replicate B	cells	count
mod_15min_C	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 15 minute grazing period; replicate C	cells	count
mod_45min_A	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 45 minute grazing period; replicate A	cells	count
mod_45min_B	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 45 minute grazing period; replicate B	cells	count
mod_45min_C	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 45 minute grazing period; replicate C	cells	count
mod_90min_A	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 90 minute grazing period; replicate A	cells	count
mod_90min_B	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 90 minute grazing period; replicate B	cells	count
mod_90min_C	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 90 minute grazing period; replicate C	cells	count
mod_24hr_A	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 24 hour grazing period; replicate A	cells	count
mod_24hr_B	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 24 hour grazing period; replicate B	cells	count
mod_24hr_C	Rhodomonas cells counted inside the grazer: moderate pCO2 level; 24 hour grazing period; replicate C	cells	count
high_15min_A	Rhodomonas cells counted inside the grazer: high pCO2 level; 15 minute grazing period; replicate A	cells	count
high_15min_B	Rhodomonas cells counted inside the grazer: high pCO2 level; 15 minute grazing period; replicate B	cells	count
high_15min_C	Rhodomonas cells counted inside the grazer: high pCO2 level; 15 minute grazing period; replicate C	cells	count
high_45min_A	Rhodomonas cells counted inside the grazer: high pCO2 level; 45 minute grazing period; replicate A	cells	count
high_45min_B	Rhodomonas cells counted inside the grazer: high pCO2 level; 45 minute grazing period; replicate B	cells	count
high_45min_C	Rhodomonas cells counted inside the grazer: high pCO2 level; 45 minute grazing period; replicate C	cells	count
high_90min_A	Rhodomonas cells counted inside the grazer: high pCO2 level; 90 minute grazing period; replicate A	cells	count
high_90min_B	Rhodomonas cells counted inside the grazer: high pCO2 level; 90 minute grazing period; replicate B	cells	count
high_90min_C	Rhodomonas cells counted inside the grazer: high pCO2 level; 90 minute grazing period; replicate C	cells	count
high_24hr_A	Rhodomonas cells counted inside the grazer: high pCO2 level; 24 hour grazing period; replicate A	cells	count
high_24hr_B	Rhodomonas cells counted inside the grazer: high pCO2 level; 24 hour grazing period; replicate B	cells	count
high_24hr_C	Rhodomonas cells counted inside the grazer: high pCO2 level; 24 hour grazing period; replicate C	cells	count

Database

Contribute Data

Dataset: Grazing experiment 4a: short term grazing by Gyrodinium
Deployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Grazing experiment 4a: short term grazing by GyrodiniumDeployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Dataset: Grazing experiment 4a: short term grazing by Gyrodinium
Deployment: Lab_Olson_B