Dataset: Grazing experiment 5: pCO2
Deployment: Lab_Olson_B

Carbonate chemistry data for low-high pCO2 acclimated Rhodomonas sp. cultures

Principal Investigator:

M. Brady Olson (Western Washington University, WWU)

Co-Principal Investigator:

Brooke Love (Western Washington University, WWU)

Suzanne Strom (Western Washington University, WWU)

Student:

Kelly Ann Still (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)

Data URL:

https://www.bco-dmo.org/dataset-deployment/669456/data

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Description

Related Reference:
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO2 induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet

Methods & Sampling

Dataset acquisition description

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. Samples for total alkalinity were taken on growth days 1, 3, 5, 7, 11, 12, 13 and 17 and preserved with HgCl2 and stored at 4° until analysis. Alkalinity was measured by gran titration using a Titrando 888, and 0.1 N HCl titrant, in a temperature controlled titration vessel. DIC samples were filtered through a 0.2 µm nylon syringe filter on the morning of the experimental day, then stored in air tight vials at 4°C until analysis within 60 days using an Apollo SciTech DIC Analyzer AS-C3 which incorporates the LI-7000 CO2/H2O Analyzer. Other parameters were calculated with CO2sys.

Data Processing Description

Dataset Processing Description

Data are unprocessed.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- nd (no data) was entered into all blank cells
- replaced spaces with underscores
- for pCO2 values, reduced digits to right of decimal to 2

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0961229	NSF Division of Ocean Sciences

Instruments

Automatic titrator

Supplied Name:

Supplied Description:

Titrando 888

Instrument Type

Generic Name: Automatic titrator

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/

Generic Description:

Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

LI-COR LI-7000 Gas Analyzer

Supplied Name: LI-7000 CO2/H2O Analyzer

Supplied Description:

Instrument Type

Generic Name: LI-COR LI-7000 Gas Analyzer

Acronym: LI-COR LI-7000

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/382/

Generic Description:

The LI-7000 CO2/H2O Gas Analyzer is a high performance, dual cell, differential gas analyzer. It was designed to expand on the capabilities of the LI-6262 CO2/ H2O Gas Analyzer. A dichroic beam splitter at the end of the optical path provides radiation to two separate detectors, one filtered to detect radiation absorption of CO2 and the other to detect absorption by H2O. The two separate detectors measure infrared absorption by CO2 and H2O in the same gas stream. The LI-7000 CO2/ H2O Gas Analyzer is a differential analyzer, in which a known concentration (which can be zero) gas is put in the reference cell, and an unknown gas is put in the sample cell.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
treatment_rep_culture_day	Treatment replicate that names the sample and the day of semi-continuous culture	unitless	replicate
alkalinity	total alkalinity of the culture material removed	micromoles/kilogram (umol/kg)	TALK
DIC	dissolved inorganic carbon	micromoles/kilogram (umol/kg)	DIC
pCO2	Partial pressure of carbon dioxide in the water body by computation from pH and alkalinity	parts per million by volume (ppmv)	pCO2

Database

Contribute Data

Dataset: Grazing experiment 5: pCO2
Deployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Grazing experiment 5: pCO2Deployment: Lab_Olson_B

Dataset acquisition description

Dataset Processing Description

Dataset: Grazing experiment 5: pCO2
Deployment: Lab_Olson_B