Dataset: Grazing experiment 5: long term grazing and growth by Oxyrrhis
Deployment: Lab_Olson_B

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).  Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.  On day 11 Rhodomonas cells from the treatments replicates were pooled then used to inoculate Oxyrrhis marina experiment treatments.  Rhodomonas were fed to Oxyrrhis at saturating food concentrations (400 µg Carbon/Liter) and maintained for 5 days in treatment CO2 conditions with daily adjustments of Rhodomonas and media to maintain a steady state Rhodomonas density. After the 5 acclimation day cell densities were again adjusted to maintain food concentration, then time zero samples were taken and fixed with acid Lugol’s for later cell counts of both Oxyrrhis and Rhodomonas.  After 24 hours another set of samples was fixed for both types of cell counts as well as counts of Rhodomonas only controls.