Dataset: TEM Pleurochrysis carterae thin section images
Deployment: Bigelow_Martinez_2015-2016

Fifty milliliter P. carterae CCMP 645 cultures grown in F/2 medium to mid-exponential phase (~ 1 × 10e6 cells ml-1) were centrifuged at 3,000 × g for 5 min and the supernatant was discarded. The cell pellets were fixed for 2 h with 2.5% glutaraldehyde in 0.2 M sodium cacodylate buffer, pH 7.2 at 4C. The pellets were washed twice in cacodylate buffer and sent to Electron Microscopy Laboratory facility at the University of Maine, Orono, ME, USA for further processing and imaging. At the microscopy facility, the sample was postfixed for 2 h in 2% OsO4 in cacodylate buffer at 4C. The cells were enrobed with 1.5% agar solution and the agar blocks were dehydrated in an ethanol series and embedded in Epon. Ultra-thin sections were double-stained with uranyl acetate and lead citrate and examined with a Phillips/FEI EM201 transmission electron microscope operated at 100 kV.

laboratory experiment