Dataset: Micro-satellite genotypes 2012
Deployment: Belize_2010

Microsatellite genotypes and geolocation data from Belize

Principal Investigator:

Peter Buston (Boston University, BU)

Co-Principal Investigator:

Cassidy C. D'Aloia (Woods Hole Oceanographic Institution, WHOI)

Contact:

Peter Buston (Boston University, BU)

BCO-DMO Data Manager:

Hannah Ake (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

An Integrative Investigation of Population Connectivity Using a Coral Reef Fish (Elacatinus Dispersal I)

Data URL:

https://www.bco-dmo.org/dataset-deployment/743601/data

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Description

Genetic and geolocation data from the Belize Barrier Reef.

Methods & Sampling

Dataset acquisition description

We collected tissue samples from 300 Elacatinus lori individuals at ten locations across the Belize barrier reef for population genetic analyses. We marked the location of each collection site with a Garmin GPSMAP 76Cx unit. Individuals were caught with slurp guns and we took a small clip from the caudal fin using scissors. All tissue samples were store in 95% EtOH. Genetic data were collected based on standard DNA extractions (DNEasy kits, Qiagen), and PCRs. Further details on all methods can be found in D’Aloia et al. (2014), Molecular Ecology.

Data Processing Description

Dataset Processing Description

Genotypes were called using GENEMAPPER v.4.0.

BCO-DMO Data Processing Notes:

-Reformatted column names to comply with naming standards
-Replaced blank cells with nd
-Columns year, lat and lon were added based on metadata provided by the PI

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1260424	NSF Division of Ocean Sciences

Instruments

None available yet

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
individual_ID	Unique ID assigned to fish	unitless	sample
site_id	Region on reef fish was from	unitless	region
Locus1_1419triBA	Number of repeats in the microsatellite allele.	count	count
Locus1_1419triBB	Number of repeats in the microsatellite allele.	count	count
Locus2_14528tetYA	Number of repeats in the microsatellite allele.	count	count
Locus2_14528tetYB	Number of repeats in the microsatellite allele.	count	count
Locus3_18144triGA	Number of repeats in the microsatellite allele.	count	count
Locus3_18144triGB	Number of repeats in the microsatellite allele.	count	count
Locus4_21378triGA	Number of repeats in the microsatellite allele.	count	count
Locus4_21378triGB	Number of repeats in the microsatellite allele.	count	count
Locus5_23415tetRA	Number of repeats in the microsatellite allele.	count	count
Locus5_23415tetRB	Number of repeats in the microsatellite allele.	count	count
Locus6_23889triBA	Number of repeats in the microsatellite allele.	count	count
Locus6_23889triBB	Number of repeats in the microsatellite allele.	count	count
Locus7_25362triGA	Number of repeats in the microsatellite allele.	count	count
Locus7_25362triGB	Number of repeats in the microsatellite allele.	count	count
Locus8_25632tetRA	Number of repeats in the microsatellite allele.	count	count
Locus8_25632tetRB	Number of repeats in the microsatellite allele.	count	count
Locus9_25745tetBA	Number of repeats in the microsatellite allele.	count	count
Locus9_25745tetBB	Number of repeats in the microsatellite allele.	count	count
Locus10_29109tetBA	Number of repeats in the microsatellite allele.	count	count
Locus10_29109tetBB	Number of repeats in the microsatellite allele.	count	count
Locus11_6231tetBA	Number of repeats in the microsatellite allele.	count	count
Locus11_6231tetBB	Number of repeats in the microsatellite allele.	count	count
Locus12_6266triYA	Number of repeats in the microsatellite allele.	count	count
Locus12_6266triYB	Number of repeats in the microsatellite allele.	count	count
Locus13_6326tetYA	Number of repeats in the microsatellite allele.	count	count
Locus13_6326tetYB	Number of repeats in the microsatellite allele.	count	count
Locus14_985tetGA	Number of repeats in the microsatellite allele.	count	count
Locus14_985tetGB	Number of repeats in the microsatellite allele.	count	count
year	Year of sampling	unitless	year
lat	Latitude for site location	decimal degrees	lat
lon	Longitude for site location	decimal degrees	lon

Database

Contribute Data

Dataset: Micro-satellite genotypes 2012
Deployment: Belize_2010

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Micro-satellite genotypes 2012Deployment: Belize_2010

Dataset acquisition description

Dataset Processing Description

Dataset: Micro-satellite genotypes 2012
Deployment: Belize_2010