Dataset: Hydrothermal vent porewater nutrients - TN TC DOC DIC DON
Deployment: AT42-05_Alvin_Dives

Alvin pushcores dedicated to nutrient analyses were sectioned to recover the 0-6cm, 6-12, and 12-18cm fractions, or the 0-10, 10-20, and 20-30cm fractions. Two ~40ml samples of sediment were placed in 50 ml Falcon tubes and were centrifuged at 3000 rpm for 15 minutes to separate porewater from the sediment.

TC and TN: The sediment cakes were analyzed for %Total Nitrogen (TN) and %Total Carbon (TC).

DIC, DOC, DON: 10 ml of the porewater was collected into septa vials for dissolved inorganic carbon (DIC), dissolved organic carbon (DOC), and dissolved organic nitrogen (DON). The headspace was flushed with nitrogen and the samples were stored inverted at 4oC.

All porewater analyses were performed at Louisiana State University Wetland Biogeochemistry Analytical Services.;