Dataset: Chlorophyll data from daily sampling at the Santa Monica Pier
Deployment: SPOT_SMP

View Data: For data, See Dataset Metadata Page: https://www.bco-dmo.org/dataset/778653

Principal Investigator:

David Caron (University of Southern California, USC)

Co-Principal Investigator:

Jennifer Beatty (University of Southern California, USC)

Julie V. Hopper (University of Southern California, USC)

Gerid A. Ollison (University of Southern California, USC)

Brittany P. Stewart (University of Southern California, USC)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Protistan, prokaryotic, and viral processes at the San Pedro Ocean Time-series (SPOT)

Version:

Deployment Synonyms:

SPOT_SMP_2018, SPOT_SMP_2019

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Description

Daily Chlorophylla (ug/L) data from surface waters off of the Santa Monica Pier, California from April 13th to May 5th 2019 as part of the San Pedro Ocean Time-series (SPOT).

Methods & Sampling

Dataset acquisition description

Chlorophyll Extraction by Non-Acidification Method
Whole seawater collected from Santa Monica Pier, CA by bucket off pier. Portion of whole seawater placed in dark for chlorophyll processing. Bottle shaken, then filtered on to GFF filter (Glass Microfiber Filter, 0.7um, 25mm,Whatman) using tower rig and gast pump vacuum. Triplicates taken for each day. Wrapped in foil and flash frozen in liquid nitrogen to preserve. Stored in lab in -80C freezer till processed.
In Lab processing: using glass cuvette (Disposable Culture Tubes, Borosilicate Glass, 12x75mm, VWR), place individual filter inside, add 4ml 100% Acetone, and cap. Ensure filter is submerged in acetone. Wrap all samples in foil and place in -20C freezer for 18-24hr. Between 18-24hrs remove samples and thaw for 30 minutes, keep in dark. Using Turner Design Fluorometer, calibrate using stored standard. For samples, invert sample multiple times, uncap, using metal spatula remove filter, use Kimwipe to clean off outside of cuvette, place in fluorometer and record both raw fluoresce and direct concentration. Dilution may be necessary if raw fluoresce is above 250 RFU.

Data Processing Description

Dataset Processing Description

Chlorophyll data was processed in Microsoft Word Excel 2011, for calibration correction on samples and averaging triplicate values.

BCO-DMO data manager processing notes:
* column names modified to remove spaces, units, and parentheses. Column names, descriptions and units can be found in the "Parameter Description" section.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1737409	NSF Division of Ocean Sciences

Instruments

Turner Designs 700 Laboratory Fluorometer

Supplied Name: Turner Design Instruments Model# 7200-000

Supplied Description:

Instrument Type

Generic Name: Turner Designs 700 Laboratory Fluorometer

Acronym: TD-700

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0510/

Generic Description:

The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Date	Date (local,UTC-7) in format m/d/yyyy	unitless	date
SAMPLE	Sample name	unitless	sample
Chl_Concentration	Chlorophyll a concentration	micrograms per liter (µg/l)	chl_a
Lat	Latitude (south is negative)	decimal degrees	lat
Lon	Longitude (west is negative)	nd	no_bcodmo_term

Database

Contribute Data

Dataset: Chlorophyll data from daily sampling at the Santa Monica Pier
Deployment: SPOT_SMP

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Chlorophyll data from daily sampling at the Santa Monica PierDeployment: SPOT_SMP

Dataset acquisition description

Dataset Processing Description

Dataset: Chlorophyll data from daily sampling at the Santa Monica Pier
Deployment: SPOT_SMP