Dataset: HPLC Pigments
Deployment: SAV-16-22

HPLC pigment data from samples collected during SAV cruises 2015-2017

Principal Investigator:

Peter Lee (College of Charleston - Hollings Marine Lab, CoC-HML)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

RUI: Vitamin B12 and nitrogen regulation of oceanic dimethylsulfoniopropionate and dimethylsulfide (B12 Impacts on DMSP)

Data URL:

https://www.bco-dmo.org/dataset-deployment/816226/data

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Description

HPLC pigment data collected during several R/V Savannah cruises conducted from 2015 to 2017 along a transect from shelf waters to oligotrophic waters in the South Atlantic Bight off the coast of Georgia (Navy Op Area NA06).

Methods & Sampling

Dataset acquisition description

Algal HPLC samples were collected by filtration under low vacuum through GF/F filters and frozen at -80C for on-shore analysis. Samples were extracted in acetone and analyzed using an Agilent 1100 HPLC system equipped with autosampler, photodiode array, and fluorescence detectors. The gradient elution program utilized was a slight modification of the Zapata et al. method (2000). Complete details of the HPLC method are described in DiTullio and Geesey 2003.

Data Processing Description

Dataset Processing Description

Pigment concentrations were determined using standard peak integration procedures with Agilent's ChemStation (version B.03.02), and entered into Microsoft Excel spreadsheets for submission to BCO-DMO. Pigment concentrations (ng/L) reported are: chlorophyll c3, chlorophyllide, magnesium-2,4-divinyl phaeoporphyrin a5 monomethyl ester, chlorophyll c2, chlorophyll c1, peridinin, 19-prime butanoyloxyfucoxanthin, fucoxanthin, neoxanthin, prasinoxanthin, violaxanthin, 19-prime hexanoyloxyfucoxanthin, diadinoxanthin, cis-fucoxanthin, alloxanthin, diatoxanthin, monadoxanthin, zeaxanthin, lutein, crocoxanthin, chlorophyll b, chlorophyll c2-like, chlorophyll a-like, divinyl chlorophyll a, chlorophyll a, pheophytin a, carotene-alpha and carotene-beta.

BCO-DMO Processing:
- renamed fields;
- added date/time field in ISO8601 format.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1436458	NSF Division of Ocean Sciences

Instruments

CTD Sea-Bird 911

Supplied Name: Sea-Bird Scientific SBE 911 CTD carousel

Supplied Description:

Instrument Type

Generic Name: CTD Sea-Bird 911

Acronym: CTD SBE 911

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/

Generic Description:

The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics.

High-Performance Liquid Chromatograph

Supplied Name: High Performance Liquid Chromatograph (HPLC) Agilent 1100

Supplied Description:

High Performance Liquid Chromatograph (HPLC) Agilent 1100 equipped with autosampler, photodiode array, and fluorescence detectors.

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

Niskin bottle

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Sea-Bird SBE 25 Sealogger CTD

Supplied Name: SBE-25 CTD

Supplied Description:

Instrument Type

Generic Name: Sea-Bird SBE 25 Sealogger CTD

Acronym: CTD SBE 25

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0040/

Generic Description:

The Sea-Bird SBE 25 SEALOGGER CTD is battery powered and is typically used to record data in memory, eliminating the need for a large vessel, electrical sea cable, and on-board computer. All SBE 25s can also operate in real-time, transmitting data via an opto-isolated RS-232 serial port. Temperature and conductivity are measured by the SBE 3F Temperature sensor and SBE 4 Conductivity sensor (same as those used on the premium SBE 9plus CTD). The SBE 25 also includes the SBE 5P (plastic) or 5T (titanium) Submersible Pump and TC Duct. The pump-controlled, TC-ducted flow configuration significantly reduces salinity spiking caused by ship heave, and in calm waters allows slower descent rates for improved resolution of water column features. Pressure is measured by the modular SBE 29 Temperature Compensated Strain-Gauge Pressure sensor (available in eight depth ranges to suit the operating depth requirement). The SBE 25's modular design makes it easy to configure in the field for a wide range of auxiliary sensors, including optional dissolved oxygen (SBE 43), pH (SBE 18 or SBE 27), fluorescence, transmissivity, PAR, and optical backscatter sensors. More information from Sea-Bird Electronics: http:www.seabird.com.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise_ID	Cruise Identifier	unitless	cruise_id
Station	Station number	unitless	station
UTC_Date	Start date for sample collection; Coordinated Universal Time; Format: MM/DD/YYYY	unitless	date
UTC_Time	Start time for sample collection; Coordinated Universal Time; Format: hh:mm:ss	unitless	time
ISO_DateTime_UTC	Start date and time of sample collection formatted to ISO8601 standard: YYYY-MM-DDThh:mm:ssZ	unitless	ISO_DateTime_UTC
Latitude	Start latitude for sample collection; Negative = South	decimal degrees North	lat
Longitude	Start longitude for sample collection; Negative = West	decimal degrees East	lon
Bottom_Depth	Bottom depth from ship's sonar	meters (m)	depth_w
CTD_bottle	Niskin bottle number from CTD rosette	unitless	bottle
Depth	Sample depth from CTD readout	meters (m)	depth
Chl_c3	Chlorophyll c3 concentration	nanograms per liter (ng/L)	chl_c3
Chl_ide	Chlorophyllide concentration	ng/L	chlide
MgDVP	Magnesium-2,4-divinyl phaeoporphyrin a5 monomethyl ester concentration	ng/L	mg_dvp_a5
Chl_c2	Chlorophyll c2 concentration	ng/L	chl_c2
Chl_c1	Chlorophyll c1 concentration	ng/L	chl_c1
Peridinin	Peridinin concentration	ng/L	peridinin
But_19	19-prime butanoyloxyfucoxanthin concentration	ng/L	fucox_but
Fuco	Fucoxanthin concentration	ng/L	fucox
Neo	Neoxanthin concentration	ng/L	neox
Prasino	Prasinoxanthin concentration	ng/L	prasinox
Viola	Violaxanthin concentration	ng/L	violax
Hex_19	19-prime hexanoyloxyfucoxanthin concentration	ng/L	fucox_hex
DD	Diadinoxanthin concentration	ng/L	diadinox
cis_fuco	Cis-fucoxanthin concentration	ng/L	cis_fucox
Allo	Alloxanthin concentration	ng/L	allox
Diato	Diatoxanthin concentration	ng/L	diatox
Monad	Monadoxanthin concentration	ng/L	monad
Zeax	Zeaxanthin concentration	ng/L	zeax
Lutein	Lutein concentration	ng/L	lutein
Croco	Crocoxanthin concentration	ng/L	crocox
Chl_b	Chlorophyll b concentration	ng/L	chl_b
Chl_c2_like	Chlorophyll c2-like concentration	ng/L	pigment_conc
Chl_a_like	Chlorophyll a-like concentration	ng/L	pigment_conc
DV_Chl_a	Divinyl-chlorophyll a concentration	ng/L	chl_a2
Chl_a	Chlorophyll a concentration	ng/L	chl_a
Ph_tin	Pheophytin a concentration	ng/L	p_phytin_a
a_Car	Carotene-alpha concentration	ng/L	carotene_a
b_Car	Carotene-beta concentration	ng/L	carotene_b

Database

Contribute Data

Dataset: HPLC Pigments
Deployment: SAV-16-22

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: HPLC PigmentsDeployment: SAV-16-22

Dataset acquisition description

Dataset Processing Description

Dataset: HPLC Pigments
Deployment: SAV-16-22