Dataset: Gulf of Mexico CTD Fluorometer
Deployment: NF1704

CTD Fluorometer

View Data: For data, See Dataset Metadata Page: https://www.bco-dmo.org/dataset/835566

Principal Investigator:

Karen E. Selph (University of Hawaiʻi at Mānoa)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Mesoscale variability in nitrogen sources and food-web dynamics supporting larval southern bluefin tuna in the eastern Indian Ocean (BLOOFINZ-IO)

Effects of Nitrogen Sources and Plankton Food-Web Dynamics on Habitat Quality for the Larvae of Atlantic Bluefin Tuna in the Gulf of Mexico (GoMex Tuna Foodweb B)

Version:

Deployment Synonyms:

BLOOFINZ-GoM

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Description

Methods & Sampling

Dataset acquisition description

Samples for HPLC chlorophyll were collected pre-dawn from Niskin bottles mounted on a 24-place rosette system equipped with a Seabird SBE911 CTD and a Seapoint fluorometer. Seapoint fluorometer data (volts) for chlorophyll a is compared with discrete HPLC bottle data.

HPLC samples (2.2-L) were collected for pigment analyses by high-pressure liquid chromatography (HPLC) at the Horn Point Analytical Services Laboratory (University of Maryland Center for Environmental Science). Pigment samples (2.2-L) were filtered onto GF/F filters, frozen in LN2 and stored at -85°C until extracted, and analyzed using an automated 1100 HPLC system with Agilent temperature-controlled autosampler, Peltier temperature-controlled column oven compartment, PDA detector and ChemStation software. The HPLC method uses a C8 column and a reversed phase, methanol-based solvent system (Van Heukelem and Thomas, 2001; Hooker et al., 2012). MVCHLa and DVCHLa are detected at 665 nm. Carotenoid and xanthophyll accessory pigments are detected at 450 nm.

Data Processing Description

Dataset Processing Description

BCO-DMO Processing Notes:
- data submitted in Excel file "GoM CTD Upcast data.csv" extracted to csv
- added conventional header with dataset name, PI name, version date
- renamed columns to conform with BCO-DMO naming conventions (removed spaces and special characters)
- formatted Date to ISO (yyyy-mm-dd)
- replaced blank cells with no data value 'nd', the default missing data identifier in the BCO-DMO system.
- removed blank rows

More information about this dataset deployment

Funding

Award Number	Funding Source
NA16NMF4320058	National Oceanic and Atmospheric Administration
OCE-1851558	NSF Division of Ocean Sciences

Instruments

CTD-fluorometer

Supplied Name: Seapoint Fluorometer

Supplied Description:

Instrument Type

Generic Name: CTD-fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A CTD-fluorometer is an instrument package designed to measure hydrographic information (pressure, temperature and conductivity) and chlorophyll fluorescence.

High-Performance Liquid Chromatograph

Supplied Name: HPLC

Supplied Description:

Automated 1100 HPLC system with Agilent temperature-controlled autosampler, Peltier temperature-controlled column oven compartment, PDA detector and ChemStation software.

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise	cruise identifier	unitless	cruise_id
Station	station identifier	unitless	station
Date	cast date, UTC	unitless	date
Lat	latitude; north is positive	decimal degrees	lat
Lon	longitude; east is positive	decimal degrees	lon
CTD_cast	cast number	unitless	cast
Cycle	Series of stations following a Lagrangian drift array, as described in Gerard et al., 2021	unitless	no_bcodmo_term
Day	sequential day of Cycle	unitless	day
Depth	depth	meters	depth
FL_VOLTS	fluorometer voltage measurement output of the CTD-mounted Seabird fluorometer	volts	fluor
CHL_BOTTLE	total chlorophyll a (monovinyl + divinyl chlorophyll a) from HPLC analyses of bottle data from the same casts as the Seabird fluorometer	micrograms/liter (ug/L)	chl_a

Database

Contribute Data

Dataset: Gulf of Mexico CTD Fluorometer
Deployment: NF1704

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Gulf of Mexico CTD FluorometerDeployment: NF1704

Dataset acquisition description

Dataset Processing Description

Dataset: Gulf of Mexico CTD Fluorometer
Deployment: NF1704