Dataset: Phyto HPLC Pigments
Deployment: NF1704

Phyto HPLC Pigments

Principal Investigator:

Michael R. Landry (University of California-San Diego Scripps, UCSD-SIO)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Mesoscale variability in nitrogen sources and food-web dynamics supporting larval southern bluefin tuna in the eastern Indian Ocean (BLOOFINZ-IO)

Deployment Synonyms:

BLOOFINZ-GoM

Data URL:

https://www.bco-dmo.org/dataset-deployment/851256/data

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Description

Methods & Sampling

Dataset acquisition description

This dataset is from CTD hydrocasts in the Gulf of Mexico from R/V Nancy Foster cruises in May 2017 and May 2018, which were part of a NOAA RESTORE project (aka: BLOOFINZ-GoM) to investigate the epipelagic marine nitrogen cycle, plankton dynamics, and impacts on growth and survival of larval Atlantic Bluefin Tuna (ABT). These data are meant to be used in inter-species, interregional comparisons to data from the BLOOFIN-IO study of larval Southern Bluefin Tuna in the Indian Ocean spawning region.

HPLC pigment samples (2.2-L) were collected from Niskin bottles mounted on a 24-place rosette system equipped with a Seabird SBE911 CTD and a Seapoint fluorometer. The samples were filtered onto GF/F filters, frozen in LN2 and stored at -85°C until analyses by the Horn Point Analytical Services Laboratory (University of Maryland Center for Environmental Science). Pigments were extracted and analyzed using an automated 1100 HPLC system with Agilent temperature-controlled autosampler, Peltier temperature-controlled column oven compartment, PDA detector and ChemStation software. The HPLC method uses a C8 column and a reversed phase, methanol-based solvent system (Van Heukelem and Thomas, 2001; Hooker et al., 2012). MVCHLa and DVCHLa are detected at 665 nm. Carotenoid and xanthophyll accessory pigments are detected at 450 nm. Concentrations were quantified from chromatograms relative to run standards using Agilent ChemStation software.

Data Processing Description

Dataset Processing Description

Data Processing:
ChemStation software.

BCO-DMO Processing:
- changed date form from MM/DD/YY to YYYY-MM-DD.

More information about this dataset deployment

Funding

Award Number	Funding Source
NA15OAR4320071	National Oceanic and Atmospheric Administration
OCE-1851558	NSF Division of Ocean Sciences

Instruments

CTD - profiler

Supplied Name: Seabird SBE911 CTD

Supplied Description:

Instrument Type

Generic Name: CTD - profiler

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/130/

Generic Description:

The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column. It permits scientists to observe the physical properties in real-time via a conducting cable, which is typically connected to a CTD to a deck unit and computer on a ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast.

This term applies to profiling CTDs. For fixed CTDs, see https://www.bco-dmo.org/instrument/869934.

Fluorometer

Supplied Name: Seapoint fluorometer

Supplied Description:

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

High-Performance Liquid Chromatograph

Supplied Name: Agilent 1100 HPLC system

Supplied Description:

Agilent 1100 HPLC system with temperature-controlled autosampler, Peltier temperature-controlled column oven compartment and PDA detector.

Instrument Type

Generic Name: High-Performance Liquid Chromatograph

Acronym: HPLC

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/

Generic Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

Niskin bottle

Supplied Name: Niskin bottles

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise	Cruise identifier	unitless	cruise_id
Station	Station number	unitless	station
Date	Sampling date (Central Standard (GMT-6)); format: YYYY-MM-DD	unitless	date
Lat	Latitude	degrees North	lat
Long	Longitude	degrees East	lon
Cycle	Cycle number	unitless	no_bcodmo_term
CTD	CTD cast number	unitless	cast
Exp	Experiment number	unitless	exp_id
Depth	Depth of sample	meters (m)	depth
Chl_c3	Chlorophyll c3	nanograms per liter (ng/L)	chl_c3
Chl_c12	Chlorophyll c1+c2	nanograms per liter (ng/L)	chl_c12
Chlide_a	Chlorophyllide a	nanograms per liter (ng/L)	chlide_a
Phide_a	Pheophorbide a	nanograms per liter (ng/L)	p_phorbide_a
Perid	Peridinin	nanograms per liter (ng/L)	peridinin
But_fuco	19??Butanoyloxy fucoxanthin	nanograms per liter (ng/L)	fucox_but
Fuco	Fucoxanthin	nanograms per liter (ng/L)	fucox
Neo	Neoxanthin	nanograms per liter (ng/L)	neox
Pras	Prasinoxanthin	nanograms per liter (ng/L)	prasinox
Viola	Violaxanthin	nanograms per liter (ng/L)	violax
Hex_fuco	19??Hexanoyloxy fucoxanthin	nanograms per liter (ng/L)	fucox_hex
Diadino	Diadinoxanthin	nanograms per liter (ng/L)	diadinox
Allo	Alloxanthin	nanograms per liter (ng/L)	allox
Diato	Diatoxanthin	nanograms per liter (ng/L)	diatox
Zea	Zeaxanthin	nanograms per liter (ng/L)	zeax
Lut	Lutein	nanograms per liter (ng/L)	lutein
DV_Chl_b	Divinyl Chlorophyll b	nanograms per liter (ng/L)	chl_b2
MV_Chl_b	Monovinyl Chlorophyll b	nanograms per liter (ng/L)	chl_b1
DV_Chl_a	Divinyl Chlorophyll a	nanograms per liter (ng/L)	chl_a2
MV_Chl_a	Monovinyl Chlorophyll a	nanograms per liter (ng/L)	chl_a1
T_Chl_a	Total Chlorophyll a = MV_Chl_a + DV_Chl_a	nanograms per liter (ng/L)	chl_a_tot
Phytin_a	Phaeophytin a	nanograms per liter (ng/L)	p_phytin
Caro	Carotene	nanograms per liter (ng/L)	carotene

Database

Contribute Data

Dataset: Phyto HPLC Pigments
Deployment: NF1704

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Phyto HPLC PigmentsDeployment: NF1704

Dataset acquisition description

Dataset Processing Description

Dataset: Phyto HPLC Pigments
Deployment: NF1704