Dataset: Zooplankton d13C and d15N of individual amino acids from RR1813
Deployment: RR1813

Zooplankton d13C and d15N of individual amino acids from RR1813

Principal Investigator:

Brian N. Popp (University of Hawaiʻi at Mānoa, SOEST)

Co-Principal Investigator:

Hilary G. Close (University of Miami Rosenstiel School of Marine and Atmospheric Science, UM-RSMAS)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Isotopic Indicators for Mechanisms of Organic Matter Degradation in the Northeast Pacific (EXPORTS) (EXPORTS Isotopes)

Deployment Synonyms:

EXPORTS

Data URL:

https://www.bco-dmo.org/dataset-deployment/868200/data

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Description

Methods & Sampling

Dataset acquisition description

Zooplankton were collected using a multiple opening-closing net and environmental sensing system (MOCNESS) on R/V Roger Revelle cruise RR1813 during August-September 2018 (EXPORTS cruise). Onboard, zooplankton were wet-sieved into different size fractions using filtered seawater and 0.2, 0.5, 1.0, 2.0, and 5.0 mm mesh sieves and frozen at -20ºC. Zooplankton were dried and each fraction ground using a mortar and pestle. Samples were weighed and amino acids were isolated and purified by hydrolysis in 6N hydrochloric acid followed by cation exchange chromatography. The purified amino acids were then treated with isopropanol and trifluoroacetic acid yielding trifluoroacetic amino acid esters. The resulting solution was further purified by liquid-liquid extraction into chloroform before being stored in a dichloromethane-trifluoroacetic acid solution, and then finally transferred into ethyl acetate just prior to mass spectrometric isotopic analysis. CSIA-AA was carried out on a gas chromatograph coupled to an isotope ratio mass spectrometer (GC-IRMS), with all samples measured in triplicate when amino acid concentrations were sufficient.

Data Processing Description

Dataset Processing Description

BCO-DMO Processing:
- converted dates to YYYY-MM-DD;
- added the ISO8601 date-time column;
- renamed fields to comply with BCO-DMO naming conventions.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1829425	NSF Division of Ocean Sciences
OCE-1830016	NSF Division of Ocean Sciences

Instruments

Gas Chromatograph

Supplied Name: gas chromatograph

Supplied Description:

CSIA-AA was carried out on a gas chromatograph coupled to an isotope ratio mass spectrometer (GC-IRMS).

Instrument Type

Generic Name: Gas Chromatograph

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/

Generic Description:

Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)

Isotope-ratio Mass Spectrometer

Supplied Name: Thermo Scientific Delta V Plus

Supplied Description:

Thermo Scientific Delta V Plus isotope ratio mass spectrometer with CC-C III combustion interface and Trace gas chromatograph.

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Isotope-ratio Mass Spectrometer

Supplied Name: Thermo Scientific MAT 253

Supplied Description:

Thermo Scientific MAT 253 isotope ratio mass spectrometer with GC Isolink combustion interface and Trace GC Ultra gas chromatograph.

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

MOCNESS

Supplied Name: Multiple opening-closing net and environmental sensing system (MOCNESS)

Supplied Description:

Multiple opening-closing net and environmental sensing system (MOCNESS) net with 1 m2 opening using 0.2 mm mesh plankton nets (see Wiebe, P. H., K. H. Burt, S. H. Boyd and A. W. Morton (1976) A multiple opening/closing net and environmental sensing system for sampling zooplankton. Journal of Marine Research 34:313-326.)

Instrument Type

Generic Name: MOCNESS

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/NETT0097/

Generic Description:

The Multiple Opening/Closing Net and Environmental Sensing System or MOCNESS is a family of net systems based on the Tucker Trawl principle. There are currently 8 different sizes of MOCNESS in existence which are designed for capture of different size ranges of zooplankton and micro-nekton Each system is designated according to the size of the net mouth opening and in two cases, the number of nets it carries. The original MOCNESS (Wiebe et al, 1976) was a redesigned and improved version of a system described by Frost and McCrone (1974).(from MOCNESS manual) This designation is used when the specific type of MOCNESS (number and size of nets) was not specified by the contributing investigator.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise	Cruise designation	unitless	cruise_id
R2R_Event	R2R catalog identifier for this cruise	unitless	event
Latitude	Latitude in decimal degrees North	decimal degrees North	lat
Longitude	Longitude in decimal degrees West	decimal degrees West	lon
ISO_DateTime_UTC	Date and time (UTC) of sampling in ISO8601 format: YYYY-MM-DDThh:mm:ssZ	unitless	ISO_DateTime_UTC
Date	Sampling date (UTC); format: YYYY-MM-DD	unitless	date
Time	Sampling time (UTC)/deployment of net; format: hh:mm:ss	unitless	time
MOCNESS_Tow	Shipboard MOCNESS tow designation	unitless	tow
DayNight	Indicates if sampling was conducted during day or night	unitless	time_of_day
MOCNESS_Net	Net number out of 10 nets	unitless	net
DepthInterval_max	maximum depth of sample	meters (m)	depth
DepthInterval_min	minimum depth of sample	meters (m)	depth
SizeFraction_min	minimum wet sieved size fraction	micrometers (um)	net_mesh
SizeFraction_max	maximum wet sieved size fraction	micrometers (um)	net_mesh
Taxa	which taxa were analyzed	text	taxon
d15N_Ala	the nitrogen isotopic composition of alanine	permil relative to AIR (‰)	no_bcodmo_term
d15N_Gly	the nitrogen isotopic composition of glycine	permil relative to AIR (‰)	no_bcodmo_term
d15N_Thr	the nitrogen isotopic composition of threonine	permil relative to AIR (‰)	no_bcodmo_term
d15N_Ser	the nitrogen isotopic composition of serine	permil relative to AIR (‰)	no_bcodmo_term
d15N_Val	the nitrogen isotopic composition of valine	permil relative to AIR (‰)	no_bcodmo_term
d15N_Leu	the nitrogen isotopic composition of leucine	permil relative to AIR (‰)	no_bcodmo_term
d15N_Iso	the nitrogen isotopic composition of isoleucine	permil relative to AIR (‰)	no_bcodmo_term
d15N_Pro	the nitrogen isotopic composition of proline	permil relative to AIR (‰)	no_bcodmo_term
d15N_Asx	the nitrogen isotopic composition of aspartic acid	permil relative to AIR (‰)	no_bcodmo_term
d15N_Met	the nitrogen isotopic composition of aspartic acid	permil relative to AIR (‰)	no_bcodmo_term
d15N_Glx	the nitrogen isotopic composition of glutamic acid	permil relative to AIR (‰)	no_bcodmo_term
d15N_Phe	the nitrogen isotopic composition of phenylalanine	permil relative to AIR (‰)	no_bcodmo_term
d15N_Tyr	the nitrogen isotopic composition of tyrosine	permil relative to AIR (‰)	no_bcodmo_term
d15N_Lys	the nitrogen isotopic composition of lysine	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Ala	the standard deviation of the nitrogen isotopic composition of alanine	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Gly	the standard deviation of the nitrogen isotopic composition of glycine	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Thr	the standard deviation of the nitrogen isotopic composition of threonine	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Ser	the standard deviation of the nitrogen isotopic composition of serine	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Val	the standard deviation of the nitrogen isotopic composition of valine	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Leu	the standard deviation of the nitrogen isotopic composition of leucine	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Ile	the standard deviation of the nitrogen isotopic composition of isoleucine	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Pro	the standard deviation of the nitrogen isotopic composition of proline	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Asx	the standard deviation of the nitrogen isotopic composition of aspartic acid	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Met	the standard deviation of the nitrogen isotopic composition of aspartic acid	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Glx	the standard deviation of the nitrogen isotopic composition of glutamic acid	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Phe	the standard deviation of the nitrogen isotopic composition of phenylalanine	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Tyr	the standard deviation of the nitrogen isotopic composition of tyrosine	permil relative to AIR (‰)	no_bcodmo_term
SD_d15N_Lys	the standard deviation of the nitrogen isotopic composition of lysine	permil relative to AIR (‰)	no_bcodmo_term
d13C_Ala	the carbon isotopic composition of alanine	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Gly	the carbon isotopic composition of glycine	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Thr	the carbon isotopic composition of threonine	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Ser	the carbon isotopic composition of serine	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Val	the carbon isotopic composition of valine	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Leu	the carbon isotopic composition of leucine	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Iso	the carbon isotopic composition of isoleucine	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Pro	the carbon isotopic composition of proline	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Asx	the carbon isotopic composition of aspartic acid	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Met	the carbon isotopic composition of aspartic acid	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Glx	the carbon isotopic composition of glutamic acid	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Phe	the carbon isotopic composition of phenylalanine	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Tyr	the carbon isotopic composition of tyrosine	permil relative to VPDB (‰)	no_bcodmo_term
d13C_Lys	the carbon isotopic composition of lysine	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Ala	the standard deviation of the carbon isotopic composition of alanine	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Gly	the standard deviation of the carbon isotopic composition of glycine	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Thr	the standard deviation of the carbon isotopic composition of threonine	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Ser	the standard deviation of the carbon isotopic composition of serine	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Val	the standard deviation of the carbon isotopic composition of valine	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Leu	the standard deviation of the carbon isotopic composition of leucine	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Ile	the standard deviation of the carbon isotopic composition of isoleucine	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Pro	the standard deviation of the carbon isotopic composition of proline	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Asx	the standard deviation of the carbon isotopic composition of aspartic acid	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Met	the standard deviation of the carbon isotopic composition of aspartic acid	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Glx	the standard deviation of the carbon isotopic composition of glutamic acid	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Phe	the standard deviation of the carbon isotopic composition of phenylalanine	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Tyr	the standard deviation of the carbon isotopic composition of tyrosine	permil relative to VPDB (‰)	no_bcodmo_term
SD_d13C_Lys	the standard deviation of the carbon isotopic composition of lysine	permil relative to VPDB (‰)	no_bcodmo_term

Database

Contribute Data

Dataset: Zooplankton d13C and d15N of individual amino acids from RR1813
Deployment: RR1813

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Zooplankton d13C and d15N of individual amino acids from RR1813Deployment: RR1813

Dataset acquisition description

Dataset Processing Description

Dataset: Zooplankton d13C and d15N of individual amino acids from RR1813
Deployment: RR1813