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deviation of oxidation rate of 15N from L-glutamate determined from conversion of the 15N label to 15N-labeled nitrite plus nitrate; BLD = Below Limit of Detection. 15N data are mean ? S.E.M. of duplicate biological replicates (see Damashek et al. 2018 for details) . Based on a propagation of error calculation; our conservative estimate of the precision of 15N measurements ?4? for samples at natural abundance and ?5.2? for samples artificially enriched with carrier 15N. Our limit of detection was similar to that reported by Santoro et al. (2013) and Beman et al. (2011).