Dataset: Invertebrate stable isotopes from samples collected at methane seeps off Southern California with the HOV Alvin from July 16-28, 2023 during R/V Atlantis cruise AT50-12

Pushcore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator. Rocks were placed into individual compartments in a biobox on the Alvin basket. Before preserving, samples were kept cold and animals were handpicked to sample tissue for stable isotope analyses. The tissue (aprox. 0.5-2.5 mg) was placed in pre weighted tin boats or combusted vials (500C overnight) and frozen at -70C. In addition to natural abundance analyses, some animals were placed into mylar bags and exposed to labeled C and N for incubation experiments. These were carried out for 3-8 days, and animals were then processed following the same method as for natural abundance samples. Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters. Filters were frozen for further processing in the laboratory.