Contributors | Affiliation | Role |
---|---|---|
Caron, David | University of Southern California (USC-HIMS) | Principal Investigator |
Chandler, Cynthia L. | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
Nanoplankton abundance and biovolume
See Platform deployments for cruise specific documentation
Parameter | Description | Units |
event | event number from event log | |
sta | station number from event log | |
sta_std | Arabian Sea standard station identifier | |
cast | CTD cast number | |
bot | CTD bottle number | |
press | sample depth reported as pressure | decibars |
pnp | phototrophic nanoplankton | cells/milliliter |
hnp | heterotrophic nanoplankton | cells/milliliter |
pnp_biov | phototrophic nanoplankton biovolume | 10^6 cubic micrometers/liter |
hnp_biov | heterotrophic nanoplankton biovolume | 10^6 cubic micrometers/liter |
Dataset-specific Instrument Name | Niskin Bottle |
Generic Instrument Name | Niskin bottle |
Dataset-specific Description | Niskin bottles were mounted on the CTD rosette. |
Generic Instrument Description | A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. |
Website | |
Platform | R/V Thomas G. Thompson |
Report | |
Start Date | 1995-01-08 |
End Date | 1995-02-05 |
Description | Purpose: Process Cruise #1 (Late NE Monsoon) Methods & Sampling PI: David Caron of: Woods Hole Oceanographic Institution dataset: Nanoplankton abundance and biovolume dates: January 09, 1995 to January 31, 1995 location: N: 22.4826 S: 10.0013 W: 57.2999 E: 68.75 project/cruise: Arabian Sea/TTN-043 - Process Cruise 1 (Late NE Monsoon) ship: Thomas Thompson David Caron WHOI Methods for nanoplankton counts Assemblages of phototrophic (pnp) and heterotrophic (hnp) nanoplankton were preserved in a final concentration of 1% formalin and stored at 4 degrees C. Samples were prepared for enumeration by epifluoresence microscopy within 24 hrs of preservation by staining with DAPI at a final concentration of 25 ug/ml (Caron, 1983; Sherr et al, 1993). Phototrophic (chloroplast-bearing) nanoplankton were distinguished from heterotrophs by the autofluoresence of chlorophyll a. Biovolumes were estimated by calculating the volume of an appropriate geometric shape (usually a sphere) from samples at 4 representative depths and then extrapolated through the water column. References Caron, D.A. (1983) Technique for enumeration of heterotrophic and phototrophic nanoplakton, using epifluoresence microscopy, and comparison with other procedures. Applied and Enviromental Microbiology, 46, 491-498. Sherr, E.B., D.A. Caron and B.F. Sherr (1993) Staining of heterotrophic protists for visualization via epifluoresence microscopy. In: Handbook of methods in aquatic microbial ecology, Kemp, P., J. Cole, B. Sherr and E. Sherr, eds. Lewis Publishers, Boca Raton, pp. 213-227. |
Website | |
Platform | R/V Thomas G. Thompson |
Start Date | 1995-03-14 |
End Date | 1995-04-10 |
Description | Methods & Sampling PI: David Caron of: Woods Hole Oceanographic Institution dataset: Nanoplankton abundance and biovolume dates: March 15, 1995 to April 07, 1995 location: N: 22.4853 S: 9.9994 W: 57.3007 E: 68.7532 project/cruise: Arabian Sea/TTN-045 - Process Cruise 2 (Spring intermonsoon) ship: Thomas Thompson David Caron WHOI Methods for nanoplankton counts Assemblages of phototrophic (pnp) and heterotrophic (hnp) nanoplankton were preserved in a final concentration of 1% formalin and stored at 4 degrees C. Samples were prepared for enumeration by epifluoresence microscopy within 24 hrs of preservation by staining with DAPI at a final concentration of 25 ug/ml (Caron, 1983; Sherr et al, 1993). Phototrophic (chloroplast-bearing) nanoplankton were distinguished from heterotrophs by the autofluoresence of chlorophyll a. Biovolumes were estimated by calculating the volume of an appropriate geometric shape (usually a sphere) from samples at 4 representative depths and then extrapolated through the water column. References Caron, D.A. (1983) Technique for enumeration of heterotrophic and phototrophic nanoplakton, using epifluoresence microscopy, and comparison with other procedures. Applied and Enviromental Microbiology, 46, 491-498. Sherr, E.B., D.A. Caron and B.F. Sherr (1993) Staining of heterotrophic protists for visualization via epifluoresence microscopy. In: Handbook of methods in aquatic microbial ecology, Kemp, P., J. Cole, B. Sherr and E. Sherr, eds. Lewis Publishers, Boca Raton, pp. 213-227. |
The U.S. Arabian Sea Expedition which began in September 1994 and ended in January 1996, had three major components: a U.S. JGOFS Process Study, supported by the National Science Foundation (NSF); Forced Upper Ocean Dynamics, an Office of Naval Research (ONR) initiative; and shipboard and aircraft measurements supported by the National Aeronautics and Space Administration (NASA). The Expedition consisted of 17 cruises aboard the R/V Thomas Thompson, year-long moored deployments of five instrumented surface buoys and five sediment-trap arrays, aircraft overflights and satellite observations. Of the seventeen ship cruises, six were allocated to repeat process survey cruises, four to SeaSoar mapping cruises, six to mooring and benthic work, and a single calibration cruise which was essentially conducted in transit to the Arabian Sea.
The United States Joint Global Ocean Flux Study was a national component of international JGOFS and an integral part of global climate change research.
The U.S. launched the Joint Global Ocean Flux Study (JGOFS) in the late 1980s to study the ocean carbon cycle. An ambitious goal was set to understand the controls on the concentrations and fluxes of carbon and associated nutrients in the ocean. A new field of ocean biogeochemistry emerged with an emphasis on quality measurements of carbon system parameters and interdisciplinary field studies of the biological, chemical and physical process which control the ocean carbon cycle. As we studied ocean biogeochemistry, we learned that our simple views of carbon uptake and transport were severely limited, and a new "wave" of ocean science was born. U.S. JGOFS has been supported primarily by the U.S. National Science Foundation in collaboration with the National Oceanic and Atmospheric Administration, the National Aeronautics and Space Administration, the Department of Energy and the Office of Naval Research. U.S. JGOFS, ended in 2005 with the conclusion of the Synthesis and Modeling Project (SMP).
Funding Source | Award |
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National Science Foundation (NSF) |