Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Parameters
• Instruments
• Deployments
• Project Information
• Program Information
• Funding

Pigments from HPLC analysis of bottle samples collected during EDDIES Weatherbird cruises

 dates:           2004 - 2005
 location:        Sargasso Sea 
 project/cruise:  EDDIES/WB0409 2004 Transect 1 (EDT1)
                  EDDIES/WB0413 2004 Transect 2 (EDT2)
                  EDDIES/WB0506 2005 Transect 1 (EDT3)
                  EDDIES/WB0508 2005 Transect 2 (EDT4)
 platform:        R/V Weatherbird II


 Change history: YYMMDD
    070308: downloaded original data from EDDIES data Web site 
            (200609 DM FINAL_corrected.xls);
            prepared for OCB database by Nancy Copley, OCB DMO
    070313: added to OCB database by Cyndy Chandler, OCB DMO
            event number is from cruise event log
    070405: per Jason Perl, units for all pigment concentrations  
            changed from microgram/kilogram to microgram/liter 
 
 PI note: 080317: Recalibrated data are expected from NASA during the second half of 2008. 

Methodology, protocols and PI-notes: NASA/TM-2003- HPLC Protocols (1 MB PDF)
J.L. Mueller, G.S. Fargion, C.R. McClain, C.C. Trees and J. Perl. 2003. "Ocean Optics Protocols For Satellite Ocean Color Sensor Validation, Revision 5, Volume V: Biogeochemical and Bio-Optical Measurements and Data Analysis Protocols". NASA Tech. Memo., NASA Goddard Space Flight Center, Greenbelt, Maryland. 43 pp. (PDF downlaoded from http://oceancolor.gsfc.nasa.gov/DOCS/)

Technician: Jason Perl (jperl@chors.sdsu.edu)
Center for Hydro-Optics & Remote Sensing
San Diego State University Research Foundation

Technician notes:
note 1: Did not have a signal for Chl_a @ 450nm (sample too dilute to get a chromatogram peak)
note 2: Did not have a signal for Chl_a @ 450nm or 436nm (sample too dilute to get a chromatogram peak)
note 3: no ancillary data; default volume filter set to 4000mL

chl_a_fluor and pheo_fluor are values from a Turner Fluorometer. Each HPLC extract is run on the Fluorometer as a second measure of Chla. Chla, MVa, DVa using the C18 HPLC method, yield a single peak for Chla, measured at 436nm and 450nm. A regression equation, between the 2 wavelengths (based on calibration curves of 100% MVa and 100% DVa), is used to give values for each pigment within a single peak. Chla @436nm is reported for comparison with legacy data (that are often only listed simply Chla).

DMO notes:
Original Excel file downloaded from EDDIES Web site: copy of original Excel file

The comments in the data object were taken from the 061127 version of file readme_jason_notes.txt.
SID_Filter and SID_PI were identical but some samples were also included another sample designation that was a consecutive SID_PI. In these cases, the consecutive SID replaced the SID_PI.

The original title of this project from the NSF award is: Collaborative Research: Impacts of Eddies and Mixing on Plankton Community Structure and Biogeochemical Cycling in the Sargasso Sea".

Prior results have documented eddy-driven transport of nutrients into the euphotic zone and the associated accumulation of chlorophyll. However, several key aspects of mesoscale upwelling events remain unresolved by the extant database, including: (1) phytoplankton physiological response, (2) changes in community structure, (3) impact on export out of the euphotic zone, (4) rates of mixing between the surface mixed layer and the base of the euphotic zone, and (5) implications for biogeochemistry and differential cycling of carbon and associated bioactive elements. This leads to the following hypotheses concerning the complex, non-linear biological regulation of elemental cycling in the ocean:

H1: Eddy-induced upwelling, in combination with diapycnal mixing in the upper ocean, introduces new nutrients into the euphotic zone.

H2: The increase in inorganic nutrients stimulates a physiological response within the phytoplankton community.

H3: Differing physiological responses of the various species bring about a shift in community structure.

H4: Changes in community structure lead to increases in export from, and changes in biogeochemical cycling within, the upper ocean.

Publications

Andrews, J.E., Hartin, C., and Buesseler, K.O.. "7Be Analyses in Seawater by Low Background Gamma-Spectroscopy.," Journal of Radioanalytical and Nuclear Chemistry, v.277, 2008, p. 253.

Andrews, J.E., Hartin, C., Buesseler, K.O.. "7Be Analyses in Seawater by Low Background Gamma-Spectroscopy," Journal of Radioanalytical and Nuclear Chemistry, v.277, 2008, p. 253.

Benitez-Nelson, C.R. and McGillicuddy, D.J.. "Mesoscale Physical-Biological-Biogeochemical Linkages in the Open Ocean: An Introduction to the Results of the E-Flux and EDDIES Programs.," Deep Sea Research II, v.55, 2008, p. 1133.

Benitez-Nelson, C.R. and McGillicuddy, D.J.. "Mesoscale Physical-Biological-Biogeochemical Linkages in the Open Ocean: An Introduction to the Results of the E-Flux and EDDIES Programs," Deep-Sea Research II, v.55, 2008, p. 1133.

Bibby, T.S., Gorbunov, M.Y., Wyman, K.W., Falkowski, P.G.. "Photosynthetic community responses to upwelling in mesoscale eddies in the subtropical North Atlantic and Pacific Oceans," Deep-Sea Research Part II: Topical Studies in Oceanography, v.55, 2008, p. 1310.

Buesseler, K.O., Lamborg, C., Cai, P., Escoube, R., Johnson, R., Pike, S., Masque, P., McGillicuddy, D.J., Verdeny, E.. "Particle Fluxes Associated with Mesoscale Eddies in the Sargasso Sea," Deep Sea Research II, v.55, 2008, p. 1426.

Carlson, C.A., del Giorgio, P., Herdl, G.. "Microbes and the dissipation of energy and respiration: From cells to ecosystems," Oceanography, v.20, 2007, p. 89.

Davis, C.S., and McGillicuddy, D.J.. "Transatlantic Abundance of the N2-Fixing Colonial Cyanobacterium Trichodesmium," Science, v.312, 2006, p. 1517.

Ewart, C.S., Meyers, M.K., Wallner, E., McGillicuddy, D.J., Carlson, C.A.. "Microbial Dynamics in Cyclonic and Anticyclonic Mode-Water Eddies in the Northwestern Sargasso Sea," Deep Sea Research II, v.55, 2008, p. 1334.

Ewart, C.S., Meyers, M.K., Wallner, E., McGillicuddy, D.J., Carlson, C.A.. "Microbial Dynamics in Cyclonic and Anticyclonic Mode-Water Eddies in the Northwestern Sargasso Sea," Deep-Sea Research II, v.55, 2008, p. 1334.

Goldthwait, S.A. and Steinberg, D.K.. "Elevated biomass of mesozooplankton and enhanced fecal pellet flux in cyclonic and mode-water eddies in the Sargasso Sea," Deep-Sea Research Part II: Topical Studies in Oceanography, v.55, 2008, p. 1360.

Greenan, B.J.W.. "Shear and Richardson number in a mode-water eddy," Deep-Sea Research Part II: Topical Studies in Oceanography, v.55, 2008, p. 1161.

Jenkins, W.J., McGillicuddy, D.J., and Lott III, D.E.. "The Distributions of, and Relationship Between 3 He and Nitrate in Eddies," Deep Sea Research II, v.55, 2008, p. 1389.

Jenkins, W.J., McGillicuddy, D.J., Lott III, D.E.. "The Distributions of, and Relationship Between 3 He and Nitrate in Eddies," Deep-Sea Research II, v.55, 2008, p. 1389.

Ledwell, J.R., McGillicuddy, D.J., and Anderson, L.A.. "Nutrient Flux into an Intense Deep Chlorophyll Layer in a Mode-water Eddy.," Deep Sea Research II, v.55, 2008, p. 1139.

Ledwell, J.R., McGillicuddy, D.J., Anderson, L.A.. "Nutrient Flux into an Intense Deep Chlorophyll Layer in a Mode-water Eddy," Deep-Sea Research II, v.55, 2008, p. 1139.

Li, Q.P. and Hansell, D.A.. "Intercomparison and coupling of MAGIC and LWCC techniques for trace analysis of phosphate in seawater," Analytical Chemica Acta, v.611, 2008, p. 68.

Li, Q.P., Hansell, D.A., McGillicuddy, D.J., Bates, N.R., Johnson, R.J.. "Tracer-based assessment of the origin and biogeochemical transformation of a cyclonic eddy in the Sargasso Sea," Journal of Geophysical Research, v.113, 2008, p. 10006.

Li, Q.P., Hansell, D.A., Zhang, J.-Z.. "Underway monitoring of nanomolar nitrate plus nitrite and phosphate in oligotrophic seawater," Limnology and Oceanography: Methods, v.6, 2008, p. 319.

Li, Q.P., Zhang, J.-Z., Millero, F.J., Hansell, D.A.. "Continuous colorimetric determination of trace ammonium in seawater with a long-path liquid waveguide capillary cell," Marine Chemistry, v.96, 2005, p. 73.

McGillicuddy, D.J., et. al.. "Eddy/Wind Interactions Stimulate Extraordinary Mid-Ocean Plankton Blooms," Science, v.316, 2007, p. 1021.

McGillicuddy, D.J., Ledwell, J.R., and Anderson, L.A.. "Response to Comment on "Eddy/Wind Interactions Stimulate Extraordinary Mid-Ocean Plankton Bloom".," Science, v.320, 2008.

McGillicuddy, D.J., Ledwell, J.R., Anderson, L.A.. "Response to Comment on "Eddy/Wind Interactions Stimulate Extraordinary Mid-Ocean Plankton Bloom"," Science, v.320, 2008.

McGillicuddy, et. al.. "Eddy/Wind Interactions Stimulate Extraordinary Mid-Ocean Plankton Blooms.," Science, v.316, 2007, p. 1021.

Mourino B., and McGillicuddy, D.J.. "Mesoscale Variability in the Metabolic Balance of the Sargasso Sea," Limnology & Oceanography, v.51, 2006, p. 2675.

The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.

The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.

The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.

The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.