Discrete phytoplankton, HPLC, flow cytometry, and nutrient data from GLOBEC NEP mesoscale cruises in the Northeast Pacific from 2000-2002 (NEP project)

Website: https://www.bco-dmo.org/dataset/3097
Version: 2010-07-23

Project
» U.S. GLOBEC Northeast Pacific (NEP)

Program
» U.S. GLOBal ocean ECosystems dynamics (U.S. GLOBEC)
ContributorsAffiliationRole
Letelier, RicardoOregon State University (OSU-CEOAS)Principal Investigator


Dataset Description

Phytoplankton, HPLC, flow cytometry, and nutrient data were collected by SLOWDROP optical profilers, underway, and CTD's. SLOWDROP optical profilers provided high resolution vertical profiles of temperature, salinity, and density along with bio-optical properties. The underway component focused on the assessment of the spatial and temporal variability in phytoplankton biomass and physiology. Discrete samples for chlorophyll fluorometric determinations, pigment analyis, partical absorption, and nutrients were collected by CTD cast.


Methods & Sampling

SLOWDROP optical profiling:
"We deployed a free-fall profiling system to provide high resolution vertical profiles of temperature, salinity, density (using Sea-Bird 911 CTD), along with bio-optical properties based on multi-wavelength absorption and attenuation (Wetlabs ac-9) as well as multi-wavelength fluorescence. These profiles were conducted as the optical drifters were recovered in order to provide water column assessments of the optical properties detected by the surface drifters. The optical instruments on the SLOWDROP profiler also provide calibration for the ac-9 and fluorometers used on the SeaSoar.

Underway optical properties:
The assessment of the spatial and temporal variability in phytoplankton biomass and physiology was achieved principally by gathering a continuous record of phytoplankton in vivo fluorescence, variable fluorescence, and water absorption and attenuation, using a 10-AU Turner Designs fluorometer, a Chelsea fast Repetition Rate fluorometer, and a Wetlabs ac-9 in a flow-through mode during SeaSoar survey periods.

CTDs:
In addition to the continuous records, discrete samples for chlorophyll fluorometric determinations, pigment analysis, particle absorption, and nutrients were collected every hour during the SeaSoar mesoscale survey (approximately every 10 nm) and every half hour during fine scale surveys. Samples for phytoplankton specific determination were collected at selected locations and preserved with formaldehyde."

(from W0005 cruise report. Other specific cruise-related acquisition information can be found with cruises W0008, T0205 and R0208)


Data Processing Description

For all parameters, 'nd' used for 'no data'.


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Data Files

File
phytoplankton.csv
(Comma Separated Values (.csv), 551.76 KB)
MD5:d04af1e3e5a7e199511537fc820a05a2
Primary data file for dataset ID 3097

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Parameters

ParameterDescriptionUnits
dataset

which cruise; which instrument

nd
year

Year

YYYY
yrday_utc

day of year; universal coordinated time; Jan. 1 = 1;

unitless
year_utc

Year (UTC)

YYYY
month_utc

Month (UTC)

mm
day_utc

Day (UTC)

dd
time_utc

time of day; universal coordinated time; 24-hour time

HHMM
lat

latitude; North is Positive; South is negative

decimal degrees
lon

longitude; East is Positive; West is negative

decimal degrees
platform

sample source

unitless
cast

Cast number

unitless
bottle

Bottle number

unitless
depth

depth the sample was taken

meters
temp

temperature

degrees C
sal

salinity

psu
flvolt

fluorescence

volts
chl_samp_no

Chlorophyll-a sample number

unitless
chl_a

Chlorophyll-a concentration from Turner AU fluorometer

micrograms/liter
phaeo

phaeopigment concentration from Turner AU fluorometer

micrograms/liter
nut_samp_no

nutrient sample number

unitless
PO4

Phosphate concentration

micromoles/liter
NO3_NO2

Nitrate plus nitrite concentration (aka 'nitrates')

micromoles/liter
SiOH_4

silicate concentration

micromoles/liter
NO2

Nitrite concentration

micromoles/liter
NH4

Ammonia concentration

micromoles/liter
NO3

nitrate concentration

micromoles/liter
HPLC_samp_no

High Performance Liquid Chromatography sample number

unitless
chl_c3

Chlorophyll-c3 concentration

micrograms/liter
cpyllda

Chlorophyllide-a concentration

micrograms/liter
chl_c1_c2

chlorophyll-c1 and -c2 concentration

micrograms/liter
fucox_but

19'-but-fucoxanthin concentration

micrograms/liter
fuco

fucoxanthin concentration

micrograms/liter
fucox_hex

19'hexanoyloxyfucoxanthin concentration

micrograms/liter
vxnthin

violaxanthin concentration

micrograms/liter
diadinox

diadinoxanthin concentration

micrograms/liter
alloxan

alloxanthin concentration

micrograms/liter
monadoxanth

monadoxanthin concentration

micrograms/liter
diatoxanth

diatoxanthin concentration

micrograms/liter
lutein

lutein concentration

micrograms/liter
zxnthin

zeaxanthin concentration

micrograms/liter
chl_b

Chlorophyll-b concentration

micrograms/liter
chla_lk_hplc

Chlorophyll-a like concentration from HPLC

micrograms/liter
chl_a_hplc

Chlorophyll-a concentration from HPLC

micrograms/liter
phaeo_hplc

phaeophytin concentration from HPLC

micrograms/liter
carot1

Carotene 1 concentration

micrograms/liter
carot2

Carotene 2 concentration

micrograms/liter
abs_samp_no

particulate absorption spectra sample number

unitless
aph

absorption coefficient due to phytoplankton at 676 nm

m - 1
phyto_samp_no

Phytoplankton sample number

unitless
flowcyt_no

flow cytometer sample number

unitless
synecho

synechococcus

cells/ml
EukPhy

eukaryotic phytoplankton

cells/ml
diatoms

diatoms

cells/ml
OBact

only bacteria - not Synechococcus

cells/ml
VHDNA

very high DNA

cells/ml
LowDNA

low DNA

cells/ml
OHiDNA

only high DNA

cells/ml
flags

comment flags: 0=no comment available; 9=comments available

unitless
comments

flag description

unitless
cruise

cruise identifier

unitless


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Instruments

Dataset-specific Instrument Name
AC 9
Generic Instrument Name
AC 9
Dataset-specific Description
The WetLabs ac-9 instrument was part of the flow-through system for gathering a continuous record of phytoplankton in vivo fluorescence, variable flourescences and water absorption and attenuation. (from cruise W0005 cruise report)
Generic Instrument Description
"The WET Labs AC-9 is a type of in-situ spectrophotometer that simultaneously determines the spectral transmittance and spectral absorption of water over nine wavelengths. The unit offers compact size, high precision, and excellent stability in providing a method for determining the absorption (a(l)) and beam attenuation (c(l)) coefficients. The AC-9 employs a 25-cm pathlength for effective measurement of the cleanest natural waters. The unit is also available in a 10-cm pathlength configuration." (more from WET Labs)

Dataset-specific Instrument Name
CTD Sea-Bird 911
Generic Instrument Name
CTD Sea-Bird 911
Dataset-specific Description
There was a CTD 911 deployed on the SLOWDROP as well as a CTD provided by the ship and used for CTD surveys.
Generic Instrument Description
The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics.

Dataset-specific Instrument Name
Fast Repetition Rate Fluorometer
Generic Instrument Name
Fast Repetition Rate Fluorometer
Dataset-specific Description
For these data, the FRRf was part of the flow-through system and used during SeaSoar surveys.
Generic Instrument Description
An FRRf is used for measuring the fluorescence of a sample of phytoplankton photosynthetic competency (Fv/Fm).

Dataset-specific Instrument Name
SLOWDROP Optical Profiler
Generic Instrument Name
SLOWDROP Optical Profiler
Dataset-specific Description
This free-falling profiling system provided high resolution vertical profiles of temperature,salinity, density (using associated CTD), along with bioptical properties. In addition, the optical instruments on the SLOWDROP profiler also provided calibration for the ac-9 and fluorometers used on the SeaSoar.
Generic Instrument Description
The SLOWDROP Optical Profiler is a free-falling instrument package that "has a Sea-Bird 911+ CTD, two multiwavelength absorption meters (WetLabs ac-9), and a multiexcitation spectrofluorometer (WetLabs SAFIRE) as its basic configuration. The package is deployed on a loose data tether, with sufficient buoyancy on the package to obtain descent rates of 15-20 cm/s. These slow descent rates allow data acquisition on centimeter spatial scales and result in multiparameter characterization of small-scale features." (Cowles, et al.,1998) References: Cowles, T.J., et al., 1998. Small-scale Planktonic Structure: Persistence and Trophic Consequences. Oceanography, Vol. 11(1), pp. 4-9.

Dataset-specific Instrument Name
Turner Designs Fluorometer -10-AU
Generic Instrument Name
Turner Designs Fluorometer 10-AU
Dataset-specific Description
This instrument was part of the flow-through system measuring in vivo fluorescence, variable fluorescence, and water absorption adn attentuation during SeaSoar survey periods. (From W0005 Cruise report.)
Generic Instrument Description
The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer. (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA)


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Deployments

W0005A

Website
Platform
R/V Wecoma
Report
Start Date
2000-05-29
End Date
2000-06-17

W0008

Website
Platform
R/V Wecoma
Report
Start Date
2000-07-29
End Date
2000-08-17
Description
Methods & Sampling
SLOWDROP optical profiling: We deployed a free-fall profiling system to provide high resolution vertical profiles of temperature, salinity, and density (using Seabird 911+ CTD), along with bio-optical properties based on multi-wavelength absorption and attenuation (WET Labs ac-9), as well as multi-wavelength fluorescence. These profiles were conducted near the optical drifters in order to provide water column assessments of the optical properties detected by the surface drifters. The optical instruments on the SLOWDROP profiler also provide calibration for the ac-9 and fluorometers used on the SeaSoar. Underway optical properties This program component focused on the assessment of the spatial and temporal variability in phytoplankton biomass and physiology. This goal was achieved principally by gathering a continuous record of phytoplankton in vivo fluorescence, variable fluorescence, and water absorption and attenuation, using a 10-AU Turner Designs fluorometer, a Chelsea fast Repetition Rate fluorometer, and a WET Labs ac-9 in a flow-through mode during SeaSoar survey periods. CTD: In addition to the continuous records, discrete samples for chlorophyll fluorometric determinations, pigment analysis, particle absorption, and nutrients were collected every 2 hours during the SeaSoar mesoscale survey (approximately every 15 nm), except when over the shelf, when samples were collected every half hour. Samples for phytoplankton specific determination were collected at selected locations and preserved with formaldehyde.

T0205

Website
Platform
R/V Thomas G. Thompson
Report
Start Date
2002-06-01
End Date
2002-06-17
Description
Methods & Sampling
CTD/Rosette: "CTD/rosette casts were made at specific stations along the survey lines when the SeaSoar system was out of the water. The CTD had dual T/C sensors, fluorometer, transmissometer, and oxygen sensor in addition to the rosette. Water samples were collected from the 10-l Niskin bottles for primary productivity, nutrients, salinity calibration and for chlorophyll extractions. Flow-through systems using near-surface seawater: We integrated T, S and chlorophyll fluorescence from the ship's flow-through system with GPS and bottom depth from the echosounder. We installed two ac-9s, a Fast Repetition Rate Fluorometer (FRRF), chlorophyll fluorometers and CDOM fluorometers into the underway flowthrough seawater system in the main lab. A vortex debubbler was connected in the seawater line. We used discrete samples from this system for analysis of particle size, nutrient concentration, and primary productivity." (from T0205 cruise report)

R0208

Website
Platform
R/V Roger Revelle
Report
Start Date
2002-07-31
End Date
2002-08-19


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Project Information

U.S. GLOBEC Northeast Pacific (NEP)


Coverage: Northeast Pacific Ocean, Gulf of Alaska


Program in a Nutshell

Goal: To understand the effects of climate variability and climate change on the distribution, abundance and production of marine animals (including commercially important living marine resources) in the eastern North Pacific. To embody this understanding in diagnostic and prognostic ecosystem models, capable of capturing the ecosystem response to major climatic fluctuations.

Approach: To study the effects of past and present climate variability on the population ecology and population dynamics of marine biota and living marine resources, and to use this information as a proxy for how the ecosystems of the eastern North Pacific may respond to future global climate change. The strong temporal variability in the physical and biological signals of the NEP will be used to examine the biophysical mechanisms through which zooplankton and salmon populations respond to physical forcing and biological interactions in the coastal regions of the two gyres. Annual and interannual variability will be studied directly through long-term observations and detailed process studies; variability at longer time scales will be examined through retrospective analysis of directly measured and proxy data. Coupled biophysical models of the ecosystems of these regions will be developed and tested using the process studies and data collected from the long-term observation programs, then further tested and improved by hindcasting selected retrospective data series.



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Program Information

U.S. GLOBal ocean ECosystems dynamics (U.S. GLOBEC)


Coverage: Global


U.S. GLOBEC (GLOBal ocean ECosystems dynamics) is a research program organized by oceanographers and fisheries scientists to address the question of how global climate change may affect the abundance and production of animals in the sea.

The U.S. GLOBEC Program currently had major research efforts underway in the Georges Bank / Northwest Atlantic Region, and the Northeast Pacific (with components in the California Current and in the Coastal Gulf of Alaska). U.S. GLOBEC was a major contributor to International GLOBEC efforts in the Southern Ocean and Western Antarctic Peninsula (WAP).



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