Dataset: larval krill pigments

Name: Larval krill studies - fluorescence and clearance from ARSV Laurence M. Gould LMG0106, LMG0205 in the Southern Ocean from 2001-2002 (SOGLOBEC project)
Published: 2010-02-03
Keywords: krill, biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.3300.1

Spatial Extent: N:-65.08 E:-68.5033 S:-68.4817 W:-75.8183

Temporal Extent: 2001-08-06 - 2002-09-09

Project:

U.S. GLOBEC Southern Ocean (SOGLOBEC)

Program:

U.S. GLOBal ocean ECosystems dynamics (U.S. GLOBEC)

Principal Investigator:

Dr Langdon Quetin (University of California-Santa Barbara, UCSB)

Dr Robin Ross (University of California-Santa Barbara, UCSB)

Contact:

Dr Robin Ross (University of California-Santa Barbara, UCSB)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2010-02-03

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Larval krill studies - fluorescence and clearance from ARSV Laurence M. Gould LMG0106, LMG0205 in the Southern Ocean from 2001-2002 (SOGLOBEC project)

Abstract:

The goal of the larval krill studies was to investigate the physiology and ecology of krill larvae associated with the pack ice and the microbial community on which they feed. During LMG0106 we occupied two 4-5 day ice stations (Robert and Billy) and sampled several other ice floes opportunistically. We conducted 10 instantaneous growth rate experiments, and 4 whole body clearance time experiments to determine gut passage time (decline in pigment content over time). We also sampled larvae at two additional sites for initial body pigment content (whole body fluorescence), and at 4 sites for condition factor. The under-ice algal community was sampled at one site. Length and stage frequency determinations were also determined. We occupied three time-series stations of approximately 1 week each, and in addition opportunistically sampled at times when other activities had priority. Our primary goal during the cruise was to occupy three ice camps or process stations with the intent of thoroughly studying the under-ice environment by SCUBA in conjunction with other projects working topside. (from cruise report LMG0205)

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2010-02-03	National Oceanographic Data Center (NODC)	0112635	2013-09-01
2010-02-03	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.3300.1	2018-12-13

Description

"Winter ecology of larval krill: quantifying their interaction with the pack ice habitat"

The goal of the larval krill studies was to investigate the physiology and ecology of krill larvae associated with the pack ice and the microbial community on which they feed.

During LMG0106 we occupied two 4-5 day ice stations (Robert and Billy) and sampled several other ice floes opportunistically. We conducted 10 instantaneous growth rate experiments, and 4 whole body clearance time experiments to determine gut passage time (decline in pigment content over time). We also sampled larvae at two additional sites for initial body pigment content (whole body fluorescence), and at 4 sites for condition factor. The under-ice algal community was sampled at one site. Length and stage frequency determinations were also determined.

We occupied three time-series stations of approximately 1 week each, and in addition opportunistically sampled at times when other activities had priority. Our primary goal during the cruise was to occupy three ice camps or process stations with the intent of thoroughly studying the under-ice environment by SCUBA in conjunction with other projects working topside. (from cruise report LMG0205)

Methods & Sampling

Using SCUBA techniques allowed us to collect larval krill as close as possible to their in situ condition. Larval krill were collected for time-based experimentation at the dive hole, for shipboard experiments and preserved for later analysis. Samples of the micro-zooplankton community were sampled with suction samplers to gain a better understanding of food available to the larvae relative to their distribution under the ice. In addition, at one of the process stations we were able to deploy drift nets through the dive holes to compare day/night differences in the zooplankton community at depths of 1 m and 10 m below the ice-water interface. (from cruise report LMG0205)

Data Processing Description

We collected enough krill for 12 Instantaneous Growth Rate (IGR) experiments, 15 collections for whole body fluorescence experiments (WBF), 11 collections for whole body clearance experiments (WBC), and 9 collections for condition factor. Measurements of length and stage frequency of the dive and net krill collections were done routinely. (from cruise report LMG0205)

Associated datasets:
larval_krill_sum - summary of experiments
larval_krill_cf - condition factor, C:N ratio
larval_krill_gr - growth study

Data Files

File
larval_krill_pig.csv (Comma Separated Values (.csv), 15.18 KB) MD5:826a9b06c5a7cc07ec0e17d1348e3fe9 Primary data file for dataset ID 3300

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Antarctic Sciences	ANT-9909933

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
LMG0106		2001-07-21	ARSV Laurence M. Gould	Daniel P. Costa (Chief Scientist)	data info
LMG0205		2002-07-29	ARSV Laurence M. Gould	Daniel P. Costa (Chief Scientist)	data info

Instruments

Hand-held plankton net

Supplied Name: Hand-net

Supplied Description:

Instrument Type

Generic Name: Hand-held plankton net

Acronym: Hand-net

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/22/

Generic Description:

A Hand-held plankton net is a fine-meshed net designed for sampling microzooplankton, mesozooplankton or nekton.

Manual Biota Sampler

Supplied Name: SCUBA

Supplied Description:

Instrument Type

Generic Name: Manual Biota Sampler

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/90/

Generic Description:

"Manual Biota Sampler" indicates that a sample was collected in situ by a person, possibly using a hand-held collection device such as a jar, a net, or their hands. This term could also refer to a simple tool like a hammer, saw, or other hand-held tool.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
cruiseid	cruise identification	text	cruiseid
year	year of experiment in YYYY format	unitless	year
sample_id	sample identification: WBC=whole body clearance expt.; WBF=whole body fluorescence on collection	alpha-numeric	no_bcodmo_term
lat	latitude, in decimal degrees, North is positive, negative denotes South	decimal degrees	lat
lon	longitude, in decimal degrees, East is positive, negative denotes West	decimal degrees	lon
day_local	day of month, local time		day_local
month_local	month, local time		month_local
time_local	time of day, local time, using 2400 clock format		time_local
yrday_local	local day and decimal time, as 326.5 for the 326th day of the year, or November 22 at 1200 hours (noon)		yrday_local
time_sample	time of sampling for pigment content after collection; decline of pigment content with time was used to calculate time to clear the gut of pigment.	minutes	no_bcodmo_term
pigment_content	pigment content	micrograms total chl/grams wet weight	no_bcodmo_term
stage_id	stage development index of larvae in sample (furcilia = F1-6 = 1-6, juvenile = J=7)		no_bcodmo_term
wet_weight	average wet weight/larvae in sample	mg	no_bcodmo_term