R/V Kilo Moana

2007-08-09

C-MORE BLOOMER (BLOOM Ecological Reconnaissance) C-MORE 2 cruise C-MORE August 2007 cruise objectives and logistics downloaded from C-MORE site 'Cruise objectives' document, 14 September 2009 GENERAL CRUISE OBJECTIVES The primary goal this year will be the characterization of the microbial assemblage and biogeochemical fluxes associated to summer increases in cyanobacterial biomass in the vicinity of Station ALOHA. This characterization will be compared to a sampling site where no biomass increase is detected. In addition, we will try to establish transects across a bloom region, or try to sample distinct areas where blooms are detected from remote sensing and SeaGliders, to assess the spatial heterogeneity of these blooms. GENERAL CRUISE PLAN: August 8th: Loading day August 9th, 8:00 Departure from Snug. 1st scenario: If a boom is remotely detected within 100km of Station ALOHA August 9th to August 10th at 5AM: Transit to the bloom station August 10th to August 13th in the morning: Sample and carry experiments within the bloom (considers the deployment of sediment traps for at least 72 hours on August 10th and carrying on deck incubation time series for 5 days [August 15th]) August 13th noon to August 14th evening: Series of stations to characterize the spatial heterogeneity of the bloom. August 14th evening to August 15th 5AM: Transit toward Station ALOHA or a site within 100km of this site not displaying high accumulation of chlorophyll in surface waters. August 15th to August 18th in the morning: Sample and carry experiments outside the bloom. August 19th is left as a buffer and could be used to revisit the sampling site. August 20th early morning - noon: start transit back to Honolulu. 2nd scenario: If blooms are not detected in the vicinity of Station ALOHA: August 9th to August 10th at 5AM: Transit to 24N, 158W where increase sea surface chlorophyll concentration was observed on July 18 to 28. This location could change once we have developed the full MODIS chlorophyll statistics for the month of July for the study region. We will use these statistics to assess the station that has had the largest change in chlorophyll concentration as well as the station that has not seen significant chlorophyll fluctuation within 100 to 200km radius north of Station ALOHA. These will represent our primary sampling sites, replacing the bloom and non bloom sites in the 1st scenario. As in the first scenario, we will devote August 13th and August 14th to assess the spatial heterogeneity of the sampling region. 3rd scenario: A bloom develops during the cruise. We will modify the cruise plan accordingly in order to characterize the bloom evolution. C-MORE 2 BLOOMER Cruise Reports Cruise reports available from the C-MORE ftp site: ftp://ftp.soest.hawaii.edu/dkarl/cmore/Cruise_Reports/bloomer1/ each investigator contributed a separate report. Related information sources from the C-MORE Web Site: Homepage: http://cmore.soest.hawaii.edu/cruises/cmore_2/index.htm Data: http://hahana.soest.hawaii.edu/cmorebloomer/cmorebloomer.html Cruise track: http://hahana.soest.hawaii.edu/cmorebloomer/cm2LocMap.gif Cruise objectives: http://hahana.soest.hawaii.edu/cmorebloomer/cmore_2_objectives_logistics... Cruise event sheet: http://hahana.soest.hawaii.edu/cmorebloomer/cmore_2_final_master_event_s... Cruise information and original data are available from the NSF R2R data catalog.

Methods & Sampling
# C-MORE BLOOMER core data # LMO, University of Hawaii # Dave Karl # original file: LMO_bloomer1.txt # submitted to BCO-DMO: November 30, 2010 # corrected longitude signs: February 20, 2012  

R/V Kilo Moana

2008-08-25

Preliminary Cruise Report from: http://cmore.soest.hawaii.edu/cruises/super/cruisereport.htm C-MORE science and volunteer crew reported to Snug Harbor at 0630 on Monday, 25 August, 2008. After fueling the ship, the R/V Kilo Moana departed from Honolulu, HI at approximately 1630. Starting at 2200 on this first day and for the duration of the cruise, daily and nightly underway samples were collected from the ship's flow-through system. Water collected from this system was processed for particulates, nutrients, ATP, chlorophyll, and a suite of other analyses for contextual data. The cruise track began with a northeasterly course from Oahu to 34° N, 151° W. Six stations were visited before heading east, approximately tracing 35° N latitude, along which 10 additional stations were taken. This track took us 27 hours off of the great circle path between Honolulu and Port Hueneme. The total distance of the sample transect was 2115 km. The first station consisted of a single CTD cast at 1300 on Tuesday, 26 August to collect water for a mixing experiment (mixing deep water with surface water to change nutrient concentrations). From Wednesday, 27 August to Monday, 1 September, two to three stations were visited per day, during which the manta trawl was deployed for 1.5 hours, the CTD rosette was cast to the deep chlorophyll maximum, and the HyperPro profiler and LISST particle analyzer were deployed to approximately 125m depth. Upon recovery of the manta trawl, the net was rinsed with sea water, and the cod end was detached and placed in a bucket on deck. The cod end was then taken to Lab 2, where the contents were sieved through three filters of the following mesh sizes: 5mm, 2mm, and 0.2mm. Large pieces that were not kept for later use were measured and photographed (the upper size limit for whether a sample was retained was determined by the size of the largest storage containers). The presence and abundance of fauna collected in the net were recorded. The metazoan community consisted primarily of Valella valella, Porpita porpita, Halobates, Janthina, isopods, copepods, amphipods, and small crabs. In the following summary of sample allocations, the "large" size class refers to plastic pieces >5mm; "medium" refers to 2-5mm sized pieces, and "small" refers to 0.2-2mm sized pieces. For each sample, 30-100 pieces of plastic were collected from the large and medium size classes for DNA and RNA analyses. For chlorophyll extractions, 3 large, 6 medium, and 30 small pieces were placed in acetone and refrigerated (each size class was divided into 3 tubes, for a total of 9 chlorophyll samples per station). For ATP, 5 large, 15 medium, and 50 small pieces were boiled in TRIS buffer and then frozen (each size class was divided into 5 tubes, yielding a total of 15 ATP tubes per station). From 6 of the 14 trawl collections,between 14-19 large and medium pieces were used for incubation experiments. The remaining plastic pieces were sorted by size class and stored in 5% formalin. All of the 2-5mm and >5mm sized pieces were counted, and as many of the 0.2-2mm sized pieces were counted as time allowed. In addition to these collections, an incubation experiment was conducted in which microbial processes were examined in treatments without plastic particles, with sterilized plastic particles, and with "in situ" (non-sterilized) plastic particles. The combined density of plastic particles in the 2-5mm and >5mm size classes ranged from 0.35-3.71 pieces m-3 across all sampling stations. Integrated over the top 0.5m of the ocean, the particle concentrations along the transect ranged from 174,000 to 1.85 million plastic fragments km-2. Related information from the C-MORE SUPER cruise Web site: Homepage: http://cmore.soest.hawaii.edu/cruises/super/index.htm Cruise Report: http://cmore.soest.hawaii.edu/cruises/super/cruisereport.htm Science plan: http://cmore.soest.hawaii.edu/cruises/super/science.htm Data: http://hahana.soest.hawaii.edu/cmoresuperhicat/superhicat.html Chief Scientist Report: http://hahana.soest.hawaii.edu/cmoresuperhicat/SUPER_HI-CAT_Chief_Scient... Cruise track: http://hahana.soest.hawaii.edu/cmoresuperhicat/super1track.gif Cruise plan: http://hahana.soest.hawaii.edu/cmoresuperhicat/SUPER_HI-CAT_final_cruise... Cruise information and original data are available from the NSF R2R data catalog.

Methods & Sampling
# C-MORE SUPER core data # LMO, University of Hawaii # Dave Karl # original file: LMO_super1.txt # submitted to BCO-DMO: November 30, 2010 # corrected longitude signs: February 20, 2012  

R/V Kilo Moana

2007-04-19

The BULA cruise, a transect from Suva, Fiji to Honolulu, Hawaii was the inagural cruise of the Center for Microbial Oceanography: Research and Education (C-MORE). Some of the many goals were: (1) to identify prominent trends in plankton biomass, biomass structure, and elemental stoichiometry, (2) to examine latitudinal variability in upper ocean concentrations of colored dissolved organic matter and trace metal ligands, (3) to isolate new Prochlorococcus strains, (4) to optically determine upper ocean biogeochemical variables, (5) to study the distribution, production and loss rates of dissolved hydrogen and its relationship to nitrogen fixation, (6) to study viral diversity along biogeochemical gradients, (7) to assay spatial distributions of microbial community structure based on rRNA fingerprinting and sequencing, and (8) to assess spacial variability in photophysiological responses to photoautotrophs. Original sources available from C-MORE Web Site: BULA Home page: http://cmore.soest.hawaii.edu/cruises/bula/index.htm BULA Data: http://hahana.soest.hawaii.edu/cmorebula/cmorebula.html Cruise track: http://hahana.soest.hawaii.edu/cmorebula/bula1track.gif Cruise log: http://hahana.soest.hawaii.edu/cmorebula/CMOREBULA_Cruise_Log.pdf (sample log sheets) Cruise activities: http://hahana.soest.hawaii.edu/cmorebula/Cruise_activities.pdf (Cruise Report) Cruise summary: ftp://ftp.soest.hawaii.edu/dkarl/cmore/cruise.summaries/bula1.sum (station/cast locations) Cruise information and original data are available from the NSF R2R data catalog.

Methods & Sampling
# C-MORE BULA core data # LMO, University of Hawaii # Dave Karl # original file: LMO_bula1.txt # submitted to BCO-DMO: November 30, 2010 # longitude signs corrected: February 20, 20120  

R/V Kilo Moana

2008-08-14