Dataset: Biology

Name: Biology and chemistry in Arctic surface microlayer and subsurface waters from R/V Oden cruise ASCOS2008 from the High Arctic Ocean in 2008 (87degs N, 1-6degs E) (Marine Microgels project)
Published: 2012-01-04
Keywords: oceans

Cite This Dataset

Spatial Extent: N:87.5 E:10.36 S:77.95 W:-10.47

Project:

Marine microgels: A microlayer source of summer CCN in high Arctic open lead (Marine Microgels)

Program:

Arctic Summer Cloud Ocean Study (ASCOS)

Principal Investigator:

Patricia A. Matrai (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator:

Monica V. Orellana (Institute for Systems Biology, ISB)

Contact:

Patricia A. Matrai (Bigelow Laboratory for Ocean Sciences)

BCO-DMO Data Manager:

Stephen R. Gegg (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

04 January 2012

Version Date:

2012-01-04

Validated:

Yes

Current State:

Final no updates expected

Biology and chemistry in Arctic surface microlayer and subsurface waters from R/V Oden cruise ASCOS2008 from the High Arctic Ocean in 2008 (87degs N, 1-6degs E) (Marine Microgels project)

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Description

Biology and chemistry in Arctic surface microlayer and subsurface waters: DMSP, amino acids, chlorophyll-a, carbon, nitrogen, sugars, proteins, concentrations of phytoplankton and bacteria.

Related files and references:
Matrai, P. A., Tranvik, L., Leck, C. & Knulst, J. Are high Arctic microlayers a potential source of aerosol organic precursors? Mar. Chem. 108, 109-122 (2008).

Orellana M.V., P.A. Matrai, C. Leck, C.D. Rauschenberg, Allison M. Lee and E. Coz. Marine microgels as cloud condensation nuclei in the high Arctic. PNAS 108 (33) 13612-13617; published ahead of print August 8, 2011, doi:10.1073/pnas.1102457108 (2011).

Gao, Q., C. Leck, C. Rauschenberg, and P. Matrai. On the chemical dynamics of extracellular polysaccharides in the high Arctic surface microlayer.” Atmospheric Chemistry and Physics, ASCOS Special Issue, subm. (2012).

Methods & Sampling

Sampling and Analytical Methodology:

Samples were obtained during the drift phase (13 August- 1 September 2008) of the international ASCOS field campaign to the high Arctic Ocean. Surface microlayer samples were obtained with battery-operated catamaran-type vessels fitted with a rotating drum covered with a thin sheet of sodium-in-liquid-ammonia-edged (hydrophilic) Teflon film (Matrai et al. 2008). The depth of the SML sampled ranged from 2 to 41 μm. We performed SSWW sampling with acid-cleaned bottles rinsed in distilled deionised water (18 M?) at < 30 cm below the surface microlayer.

DMS and DMSP were measured with cryotrapping and gas chromatography with flame photometric detection. The dissolved fraction was obtained by gravity filtration and low volume sampling, through a Whatman GF/F filter that retained the particulate fraction (Matrai and Keller 1993).

Chlorophylls were determined according to Holm-Hansen et al. (1965).

Bulk carbohydrates (d-MCHO, d-PCHO) were quantified with the TPTZ protocol (Hung et al. 2001, 2003).

Total organic carbon samples were collected in acid-cleaned containers and stored frozen, until analysis by high temperature combustion (Knap et al. 1996), calibrated with DOC Certified Reference standards.

Particulate organic carbon and nitrogen were quantified in material collected on Whatman GF/F glass fiber filters stored frozen until laboratory analysis (Knap et al. 1996).
Individual amino acids were determined on 0.2 µm-filtered samples, with acid hydrolysis and separation achieved by using reverse phase HPLC with precolumn OPA derivitization (Mopper & Dawson 1986, Keil and Kirchman 1991).

Proteins were determined in the particulate phase, after filtration with a Whatman GF/F glass filber filter (Dortch et al. 1984, Clayton et al. 1988).

Phytoplankton and bacterioplankton were enumerated with flow cytometry (Sieracki et al. 1999), after fixation with 0.5% paraformaldehyde, flash-freezing in liquid nitrogen, and storage at -80oC.

Clayton J J, Dortch Q, Thoresen S, Ahmed S (1988) Evaluation of methods for the separation and analysis of proteins and free amino acids in phytoplankton samples. J Plankton Res 10: 341-358. [proteins]

Dortch Q, Clayton J R J, Thoresen S S, Ahmed S I (1984) Species differences in accumulation of nitrogen pools in phytoplankton. Mar Biol 81: 237-250. [proteins]

Holm-Hansen O, CJ Lorenzen, RW Holmes and JDH. Strickland. 1965. Fluorometric determination of chlorophyll. Journal du Conseil. Conseil International pour l'Exploration de la Mer 30 : 3-15.
Hung C-C et al. (2003) Distributions of carbohydrate species in the Gulf ofMexico. Mar Chem 81: 119-135. [polysacharides]

Hung C-C, Tang D, Warnken K, Santschi P H (2001) Distributions of carbohydrates, including uronic acids, in estuarine waters of Galveston Bay. Mar Chem 73: 305- 318. [mono, polysacharides]

Keil R, Kirchman D (1991) Dissolved combined aminoacids in marine waters as determined by a vapor-phase hydrolysis method. Mar Chem 33: 243-259. [aminoacids]

Knap A et al. (1996) Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. [POC, PON, TOC, TN]

Matrai P A, Keller M D (1993) Dimethylsulfide in a large-scale coccolithophore bloom in the Gulf of Maine. Cont Shelf Res 13: 831-843. [DMS, DMSP]

Matrai, P. A., Tranvik, L., Leck, C. & Knulst, J. Are high Arctic microlayers a potential source of aerosol organic precursors? Mar. Chem. 108, 109-122 (2008). [microlayer sampling]

Mopper K, Dawson R (1986) Determination of amino acids in seawater - Recent chromatographic developments and future directions. Sci Total Environ 49: 115-131. [amino acids]

Sieracki, M., T. Cucci, and J. Nicinskihttp://aem.asm.org/content/65/6/2409.abstract - aff-1 (1999). "Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures." Applied and Environmental Microbiology 65(6): 2409-2417. [phytoplankton and bacterial counts]

Data Processing Description

Data Processing:
These are the original data, derived from calibration curves, as described in the methods.

BCO-DMO Processing Notes
Generated from original .xlsx file "ASCOS-Biology_data-for BCO-DMO2.csv" contributed by Paty Matrai

BCO-DMO Edits

- Date reformatted to "YYYYMMDD"

- "NaN" replaced with "nd" for no data value
- "nd" (no data value) inserted in blank cells
- Commas in Station type converted to semicolons
- Parameter names modified to conform to BCO-DMO convention(spaces eliminated, underscores inserted, etc)

- Data values standardized to common number of decimal places reorted for data/parameter type

Data Files

File
Biology.csv (Comma Separated Values (.csv), 7.80 KB) MD5:f3344da9d9563e5db9f2c9e15219edd5 Primary data file for dataset ID 3593

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Arctic Sciences	ARC-0707513
NSF Arctic Sciences	ARC-0707555

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
ASCOS2008		2008-08-01	R/V Oden	Dr Caroline Leck (Chief Scientist) Dr Michael Tjernstrom (Chief Scientist)	data info

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Date	Date	YYYYMMDD	date
DOY	Day of Year	ddd.dd	no_bcodmo_term
Station_type	Station type description	text	no_bcodmo_term
Lat	Station latitude (South is negative)	Decimal degrees	lat
Lon	Station longitude (West is negative)	Decimal degrees	lon
true_wind_direction	True wind direction	degrees	wind_dir
true_wind_speed	True wind speed	m/s	wind_speed
depth	Depth	m	depth
DMS	dimethyl sulfide	nM	no_bcodmo_term
DMS_sd	dimethyl sulfide sdev	nM	no_bcodmo_term
DMSPp	particulate dimethylsulfoniopropionate	nM	no_bcodmo_term
DMSPp_sd	particulate dimethylsulfoniopropionate sdev	nM	no_bcodmo_term
DMSPd	dissolved dimethylsulfoniopropionate	nM	no_bcodmo_term
DMSPd_sd	dissolved dimethylsulfoniopropionate sdev	nM	no_bcodmo_term
AA_diss	total dissolved aminoacids	nM	no_bcodmo_term
AA_sd	total dissolved aminoacids sdev	nM	no_bcodmo_term
Chl	chlorophyll a	ug/l	no_bcodmo_term
Chl_sd	chlorophyll a sdev	ug/l	no_bcodmo_term
POC	particulate organic carbon	ug/l	POC
POC_sd	particulate organic carbon sdev	ug/l	no_bcodmo_term
PON	particulate organic nitrogen	ug/l	PON
PON_sd	particulate organic nitrogen sdev	ug/l	no_bcodmo_term
Tot_ndo_phyto_conc	Total nano phytoplankton concentration	Number of cells/ml	no_bcodmo_term
Tot_Bact_conc	Total bacterial concentration	Number of cells/ml	no_bcodmo_term
polysaccharides_acid	Polysaccharides acid	ug C/L	no_bcodmo_term
polysaccharides_acid_sd	Polysaccharides acid sdev	ug C/L	no_bcodmo_term
monosacharides_dissolved	Monosaccharides	uM C	no_bcodmo_term
monosaccharides_sd	Monosaccharides sdev	uM C	no_bcodmo_term
polysaccharides_dissolved	Polysaccharides total	uM C	no_bcodmo_term
Proteins_particulate	Proteins particulate	ug/l	no_bcodmo_term
proteins_sd	Proteins particulate sdev	ug/l	no_bcodmo_term
TOC	total organic carbon	uM	TOC
TN	total nitrogen	uM	no_bcodmo_term

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Dataset: Biology