Dataset: phytoplankton

Name: Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) determined by flow cytometry of samples collected on R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project)
Published: 2014-09-10
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.3901.4

Spatial Extent: N:46.6888 E:-118.316 S:21.3428 W:-160.6166

Temporal Extent: 2012-03-01 - 2013-07-27

Project:

Seasonal and decadal changes in temperature drive Prochlorococcus ecotype distribution patterns (POWOW)

Principal Investigator:

Zackary I. Johnson (Duke University)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2014-09-10

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) determined by flow cytometry of samples collected on R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project)

Abstract:

This dataset quantifies phytoplankton (Prochlorococcus, Synechococcus, and PEuks), determined by flow cytometry of samples collected on R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2014-09-10	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.3901.4	2021-12-28

Description

Phytoplankton concentrations from POWOW1 (TN277), POWOW2 (KM1301), and POWOW3 (KM1312) cruises including Prochlorococcus, Synechococcus and pico-photosynthetic eukaryotes (PEuks) determined by flow cytometry.

Methods & Sampling

Phytoplankton were enumerated using a FACSCalibur flow cytometer (Becton Dickinson) and populations characterized as previously described (Johnson et al., 2010). Briefly, cells were excited with a 488 nm laser (15 mW Ar) and inelastic forward (<15°) scatter, inelastic side (90°) scatter (SSC), green (530 ± 30 nm) fluorescence, orange fluorescence (585 ± 42 nm), and red fluorescence (> 670 nm) emissions were measured. Populations were deemed ‘phytoplankton’ if they contained red-fluorescence, i.e. had a chlorophyll signal and were subsequently assigned to specific taxa (i.e. Prochlorococcus, Synechococcus or Pico-photosynthetic eukaryotes) based on previous classification schemes (Johnson et al., 2010).

References:
Johnson, Z.I., Shyam, R., Ritchie, A.E., Lin, Y., Mioni, C., Lance, V.P. et al. (2010) The effects of iron- and light-limitation on phytoplankton communities of deep chlorophyll maxima of the Western Pacific Ocean. Journal of Marine Research 68: 1-26. doi: 10.1357/002224010793721433

Data Processing Description

Cells counts were normalized to volume sampled to determined cells per mL.

BCO-DMO edits made:
- Parameter names have been changed to conform to BCO-DMO conventions.
- month_utc, day_utc, and year were added, based on the original date field.
- Original version of POWOW1 data (dated 25 March 2013) replaced with updated version 28 May 2013; POWOW2 added 14 May 2013.
- Replaced 'NaN' with 'nd' to indicate 'no data'.

Data Files

File
phytoplankton.csv (Comma Separated Values (.csv), 62.12 KB) MD5:29f7c07c188d5a0486033cae374597b2 Primary data file for dataset ID 3901

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1031064

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
KM1301	POWOW2	2013-01-10	R/V Kilo Moana	Zackary I. Johnson (Chief Scientist) Erik Zinser (Co-Chief Scientist) Zackary I. Johnson (Contact)	data info
KM1312	POWOW3	2013-07-01	R/V Kilo Moana	Zackary I. Johnson (Chief Scientist) Erik Zinser (Co-Chief Scientist) Zackary I. Johnson (Contact)	data info
TN277	POWOW1	2012-02-29	R/V Thomas G. Thompson	Zackary I. Johnson (Chief Scientist) Erik Zinser (Co-Chief Scientist) Zackary I. Johnson (Contact)	data info

Instruments

Flow Cytometer

Supplied Description:

A FACSCalibur flow cytometer (Becton Dickinson) was used to enumerate phytoplankton.

Instrument Type

Generic Name: Flow Cytometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/

Generic Description:

Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
cruise_name	Cruise identifier (POWOW1 = TN277 = R/V Thomas G. Thompson cruise 277; POWOW2 = KM1301 = R/V Kilo Moana cruise 1301).	text	cruise_name
cast	CTD cast number.	unitless	cast
lat	Latitude in decimal degrees. Positive = North.	decimal degrees	lat
lon	Longitude in decimal degrees. Positive = East.	decimal degrees	lon
month_utc	2-digit month of year, UTC.	mm (01 to 12)	month_utc
day_utc	2-digit day of month, UTC.	dd (01 to 31)	day_utc
year	4-digit year.	YYYY	year
depth	Sample depth.	meters	depth
bot	Rosette position of the bottle.	unitless	bot
prochlorococcus	Concentration of Prochlorococcus (cells/mL).	cells per milliliter	coccus_p
synechococcus	Concentration of Synechococcus (cells/mL).	cells per milliliter	coccus_s
pico_euks	Concentration of pico-photosynthetic eukaryotes, or PEuks (cells/mL).	cells per milliliter	pico_euks
depth_w	Depth of the water (bottom depth).	meters	depth_w
time_utc	Time, in hours and minutes, UTC; 24-hour clock.	HHMM	time_utc

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Database

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Dataset: phytoplankton