Table of Contents

• Coverage
• Dataset Description
  • Methods & Sampling
  • BCO-DMO Processing Description
• Data Files
• Parameters
• Instruments
• Deployments
• Project Information
• Funding

Ammonium data from core casts, isopycnal casts, and beach casts for the HOE-DYLAN cruises.

In the summer of 2012, C-MORE conducted a "continuous" long-term field experiment at Station ALOHA to observe and interpret temporal variability in microbial processes, and the consequences for ecological dynamics and biogeochemical cycling. Special focus was given to time-space coupling because proper scale sampling of the marine environment is an imperative, but generally neglected aspect of marine microbiology.

Hawaii Ocean Experiment - Dynamics of Light and Nutrients (HOE-DYLAN)

Seawater samples were collected from Niskin bottles with acid-washed syringes to avoid contamination from air and ship fumes, and were kept frozen (-20 ºC) until analysis. Ammonium was measured at the nanomolar level with the phenol method (e.g. Hansen and Koroleff, 1999), using a flow-injection system coupled to a 2m Liquid Waveguide Capillary Cell to read the color. Low nutrient seawater was used for working standards preparation, blanks and carrier. All material and instruments was acid-washed before use. The absorbance-concentration relationship was lineal up to at least 500 nM ammonium, with a method detection limit of 3.7 nM and an error of 14% at 50 nM and 2% at 100-200 nM.

From the Hoe-DYLAN cruise summary file, the correct ‘date’ value for Station 2 and Cast 33 is 20120828, so a correction was made to replace the bad dates for Station 2 and Cast 33 to 20120828. This replaces errors in the day number that are varying for Station 2 and Cast 33 and that are greater than 31.

From the Hoe-DYLAN cruise summary file, the correct ‘time’ value for Station 2 and Cast 33 is 0154, so a correction was made to replace the bad times for Station 2 and Cast 33 to 0154. This replaces errors in the minutes that are varying for Station 2 and Cast 33 and that are greater than 59.

The time parameter is GMT, so the date and time columns in the submitted file were combined to create an ISO datetime column named ISO_DateTime_UTC of format YYYY-MM-DDThh:mmZ and the date and time columns removed.

The Hoe-DYLAN cruise summary file: https://hahana.soest.hawaii.edu/FTP/cmore/cruise.summaries/hoedylan9.sum includes a list of station #, cast # and corresponding date and time.

Project summary

The Center for Microbial Oceanography: Research and Education (C-MORE) is a recently established (August 2006; NSF award: EF-0424599) NSF-sponsored Science and Technology Center designed to facilitate a more comprehensive understanding of the diverse assemblages of microorganisms in the sea, ranging from the genetic basis of marine microbial biogeochemistry including the metabolic regulation and environmental controls of gene expression, to the processes that underpin the fluxes of carbon, related bioelements and energy in the marine environment. Stated holistically, C-MORE's primary mission is: Linking Genomes to Biomes.

We believe that the time is right to address several major, long-standing questions in microbial oceanography. Recent advances in the application of molecular techniques have provided an unprecedented view of the structure, diversity and possible function of sea microbes. By combining these and other novel approaches with more well-established techniques in microbiology, oceanography and ecology, it may be possible to develop a meaningful predictive understanding of the ocean with respect to energy transduction, carbon sequestration, bioelement cycling and the probable response of marine ecosystems to global environmental variability and climate change. The strength of C-MORE resides in the synergy created by bringing together experts who traditionally have not worked together and this, in turn, will facilitate the creation and dissemination of new knowledge on the role of marine microbes in global habitability.

The new Center will design and conduct novel research, broker partnerships, increase diversity of human resources, implement education and outreach programs, and utilize comprehensive information about microbial life in the sea. The Center will bring together teams of scientists, educators and community members who otherwise do not have an opportunity to communicate, collaborate or design creative solutions to long-term ecosystem scale problems. The Center's research will be organized around four interconnected themes:

• (Theme I) microbial biodiversity,
• (Theme II) metabolism and C-N-P-energy flow,
• (Theme III) remote and continuous sensing and links to climate variability, and
• (Theme IV) ecosystem modeling, simulation and prediction.

  Each theme will have a leader to help coordinate the research programs and to facilitate interactions among the other related themes. The education programs will focus on pre-college curriculum enhancements, in service teacher training and formal undergraduate/graduate and post-doctoral programs to prepare the next generation of microbial oceanographers. The Center will establish and maintain creative outreach programs to help diffuse the new knowledge gained into society at large including policymakers. The Center's activities will be dispersed among five partner institutions:

• Massachusetts Institute of Technology,
• Woods Hole Oceanographic Institution,
• Monterey Bay Aquarium Research Institute,
• University of California at Santa Cruz and
• Oregon State University

and will be coordinated at the University of Hawaii at Manoa.

Related Files:

Strategic plan (PDF file)