Dataset: Data Set 1B: Bioavailability of dissolved organic carbon produced by Thalassiosira weissflogii grown under different pCO2 and temperature conditions - Experiment OA5

Name: 1B: Bioavailability of dissolved organic carbon produced by Thalassiosira weissflogii grown under different pCO2 and temperature conditions from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Effects of High CO2 project)
Published: 2013-11-21
Keywords: biota, oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.471701.1

Spatial Extent: N:34.4126 E:-119.842 S:34.4126 W:-119.842

Temporal Extent: 2009 - 2010

Project:

Will high CO2 conditions affect production, partitioning and fate of organic matter? (OA - Effects of High CO2)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Ocean Carbon and Biogeochemistry (OCB)

Principal Investigator:

Uta Passow (University of California-Santa Barbara, UCSB-MSI)

Co-Principal Investigator:

Mark A. Brzezinski (University of California-Santa Barbara, UCSB-MSI)

Craig A. Carlson (University of California-Santa Barbara, UCSB-MSI)

Student:

Ms Anna James (University of California-Santa Barbara, UCSB-MSI)

Contact:

Uta Passow (University of California-Santa Barbara, UCSB-MSI)

BCO-DMO Data Manager:

Stephen R. Gegg (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2013-11-21

Restricted:

Release Date:

2016-09-30

Validated:

Yes

Current State:

Final no updates expected

1B: Bioavailability of dissolved organic carbon produced by Thalassiosira weissflogii grown under different pCO2 and temperature conditions from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Effects of High CO2 project)

Abstract:

Bioavailability of dissolved organic carbon produced by Thalassiosira weissflogii grown under different pCO2 and temperature conditions from UCSB Marine Science Institute Passow Lab from 2009 to 2010.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2013-11-21	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.471701.1	2020-01-24

Description

Data Set: 1B
3/14/2013
Mini Remin Exp
OA5 Bioavailability Exp 2013

Is there a significant difference in the bioavailability of organic matter produced by T. Weiss as a result of varied temperature and pCO2?

Does this organic matter become less available (higher C:N) as the phytos become more nutrient stressed?

Methods & Sampling

Treatments:
1. 20deg 400ppm 2 days post nutrient depletion
2. 20deg 400ppm 4 days post nutrient depletion
3. 20deg 400ppm 6 days post nutrient depletion
4. 20deg 1000ppm 2 days post nutrient depletion
5. 20deg 1000ppm 4 days post nutrient depletion
6. 20deg 1000ppm 6 days post nutrient depletion
7. 15deg 400ppm 2 days post nutrient depletion
8. 15deg 400ppm 4 days post nutrient depletion
9. 15deg 400ppm 6 days post nutrient depletion
10. 15deg 1000ppm 2 days post nutrient depletion
11. 15deg 1000ppm 4 days post nutrient depletion
12. 15deg 1000ppm 6 days post nutrient depletion

Set-up:
Sample:
1.4L: 80% 0.22um filtered from Jan Taucher's treatments (collected from March 9th (20deg 2day post nut depletion) to March 15th (15deg 6day post nut depletion))

0.3L: 20% 1.22um filtered campus point SW (collected 1000 on 03/16/2013)
Total volume per 2L PC bottle (treatment) = 1.5L

The total volume was combined at 1130 and then each 2L PC bottle (with 1.5L total volume) was split between duplicate 500ml PC bottles (the remaining volume was used for T0 sampling)

These bottles were then placed in the small incubator in the Chapman lab at 12 deg (same temperature of the innoclum during time of collection)

Sampling Schedule:
Time point Time (hrs) Date Time Samples Taken From what
T0 0 5/14/2013 930 DOC, FCM From 2L PC bottles
T1 5/15/2013 1230 FCM all 24
T2 5/16/2013 1210 FCM all 24
T3 3.5 5/17/2013 1100 DOC, FCM all 24
T4 5/18/2013 1400 FCM all 24
T5 5/19/2013 1030 FCM all 24
T6 148 5/20/2013 1330 FCM all 24
T7 160.75 5/21/2013 945 FCM all 24
T8 191 5/22/2013 1600 DOC, FCM all 24
TF ERH?? 6/6/2013 ERH?? DOC all 24

Data Processing Description

BCO-DMO Processing Notes
Original file: "AJames_Data.xlsx" Sheet: "OA5" contributed by Anna James
- Approx Lat/Lon of Passow Lab appended to enable data discovery in MapServer
- "nd" (no data) inserted into blank cells
- Parameter names edited to conform to BCO-DMO parameter naming conventions

Data Files

File
DataSet1b_OA5_2013.csv (Comma Separated Values (.csv), 5.04 KB) MD5:baf0ad6ae4ea659c3e72ba192b19bfa7 Primary data file for dataset ID 471701

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1041038

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
lab_UCSB_MSI_Passow	Passow Lab	2009-09-01	UCSB MSI Passow	Uta Passow (Principal Investigator)	data info

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Lab_Id	Lab Id – Lab identifier where experiments were conducted	text	laboratory
Lat	Approximate Latitude Position of Lab; South is negative	decimal degrees	lat
Lon	Approximate Longitude Position of Lab; West is negative	decimal degrees	lon
Bottle_Num	PC bottle number sampled	dimensionless	sample
Temp	Temperature	degrees C	temp
pCO2	pCO2 conditions	ppm	pCO2
days_post_nut_depletion	Days post nut depletion	days	days
DOC_T0_0	Dissolved Organic Carbon at T0 (0 hrs)	uM C	DOC
DOC_T2_70point5	Dissolved Organic Carbon at T2 (70.5 hrs)	uM C	DOC
DOC_T7_195	Dissolved Organic Carbon at T7 (195 hrs)	uM C	DOC
DOC_T10_284	Dissolved Organic Carbon at T10 (284 hrs)	uM C	DOC
DOC_TF_968point5	Dissolved Organic Carbon at TF (968.5 hrs)	uM C	DOC
FCM_T0_0	Bacteria as determined by flow cytometry at T0 (0 hrs)	cells/ml	bact_abund
FCM_T1_45	Bacteria as determined by flow cytometry at T1 45 hrs)	cells/ml	bact_abund
FCM_T2_70point5	Bacteria as determined by flow cytometry at T2 (70.5 hrs)	cells/ml	bact_abund
FCM_T3_97point5	Bacteria as determined by flow cytometry at T3 (97.5 hrs)	cells/ml	bact_abund
FCM_T4_117	Bacteria as determined by flow cytometry at T4 (117 hrs)	cells/ml	bact_abund
FCM_T5_142pont5	Bacteria as determined by flow cytometry at T5 (142.5 hrs)	cells/ml	bact_abund
FCM_T6_169point5	Bacteria as determined by flow cytometry at T6 (169.5 hrs)	cells/ml	bact_abund

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Dataset: Data Set 1B: Bioavailability of dissolved organic carbon produced by Thalassiosira weissflogii grown under different pCO2 and temperature conditions - Experiment OA5

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Database

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Dataset: Data Set 1B: Bioavailability of dissolved organic carbon produced by Thalassiosira weissflogii grown under different pCO2 and temperature conditions - Experiment OA5

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