Dataset: Paired metagenomic and metatranscriptomic data from R/V Atlantic Explorer AE1006, AE1024, Bermuda Atlantic Time Series Station (BATS), Sargasso Sea, Mar and Aug 2010 (Active bacteria in surface waters project)

Preliminary and in progressVersion 2014-04-23 (2014-04-23)Dataset Type:Unknown

Principal Investigator: Dr Barbara Campbell (Clemson University)

Co-Principal Investigator: Dr John Heidelberg (University of Southern California)

Co-Principal Investigator: David L. Kirchman (University of Delaware)

Student: Rohan Sachdeva (University of Southern California)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)


Project: Are abundant bacteria more active than rare bacteria in the Sargasso Sea? (Active bacteria in surface waters)

A combination of pyrosequencing and QPCR approaches were used to examine rRNA:rDNA ratios, BrdU incorporating cells, and transcript types and amounts in the metatranscriptome of Sargasso Sea surface water. The investigators also used Micro-FISH to examine incorporation of thymidine, leucine, and PO4. Samples were collected in 2010 during the spring phytoplankton bloom when heterotrophic bacterial production is lowest and during the peak of bacterial production in summer.


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