Dataset: picophyto_FCM_ship

Name: Picophytoplankton abundances of live samples determined by flow cytometry (shipboard analysis) on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project)
Published: 2014-06-02
Keywords: oceans

Cite This Dataset

DOI:10.1575/1912/bco-dmo.516327.1

Spatial Extent: N:10.2998 E:-86.735 S:6.6219 W:-92.9871

Temporal Extent: 2010-06-24 - 2010-07-24

Project:

Costa Rica Dome FLUx and Zinc Experiments (CRD FLUZiE)

Program:

Integrated Marine Biogeochemistry and Ecosystem Research -US (IMBER-US)

Ocean Carbon and Biogeochemistry (OCB)

Principal Investigator:

Michael R. Landry (University of California-San Diego Scripps, UCSD-SIO)

Contact:

Karen E. Selph (University of Hawaiʻi at Mānoa, SOEST)

BCO-DMO Data Manager:

Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2014-06-02

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Picophytoplankton abundances of live samples determined by flow cytometry (shipboard analysis) on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project)

Abstract:

Picophytoplankton abundances from live samples were analyzed within 1-3 hrs on a Beckman-Coulter EPIC XL flow cytometer. Cell populations include Synechococcus and Photosynthetic Eukaryotes. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2014-06-02	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.1575/1912/bco-dmo.516327.1	2019-12-29

Description

Picophytoplankton abundances from live samples were analyzed within 1-3 hrs on a Beckman-Coulter EPIC XL flow cytometer. Cell populations include Synechococcus and Photosynthetic Eukaryotes. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean.

Methods & Sampling

Samples were collected from the CTD-Rosette PVC Niskin bottles, kept on ice in the dark until analyzed within 1-3 hr on a Beckman-Coulter EPICS XL flow cytometer, equipped with one 488 nm 1 mW laser for excitation. Samples (2.2 mL) were delivered quantitatively to the instrument using an Orion syringe pump, at a rate of 0.44 mL per minute. Signals from the Chlorophyll (680±20 nm), Phycoerythrin (575±20 nm), and the forward and side scatter detectors were used to delineate Synechococcus, and Photosynthetic eukaryotes. Calibration beads (6 um and 20 um yellow-green beads) were used to normalize cellular fluorescence values and assure optimal instrument settings. Raw data files (listmode) were processed in FlowJo software (Treestar Inc.).

Data Processing Description

Raw data, representing 2.2 mL of sample, was corrected for run volume, to arrive at cellular concentrations (cells/mL).

Data Files

File
picophyto_fcm_ship.csv (Comma Separated Values (.csv), 16.12 KB) MD5:d4ec1aa3388e7afbfd34375400e89d9e Primary data file for dataset ID 516327

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0826626

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
MV1008	CRD FLUZiE	2010-06-22	R/V Melville	Michael R. Landry (Chief Scientist)	data info

Instruments

Flow Cytometer

Supplied Name: Beckman-Coulter Altra flow cytometer

Supplied Description:

Samples were run on a Beckman-Coulter Altra flow cytometer, equipped with one 488 nm 1W laser for excitation.

Instrument Type

Generic Name: Flow Cytometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/

Generic Description:

Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)

Niskin bottle

Supplied Description:

Samples were collected from the CTD-Rosette PVC Niskin bottles.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
event	Number referring to the particular activity (event) on the FluZiE cruise.	integer	event
cast	CTD Cast number from the FluZiE cruise.	integer	cast
niskin	Niskin bottle that the sample was taken from.	integer	bot_Nis
date_local	Date of CTD cast (local time zone of UTC -6). in the format mmddyyyy	unitless	date
lon	Longitude in degrees East.	decimal degrees	lon
lat	Latitude in degrees North.	decimal degrees	lat
depth	Sample depth.	meters	depth
cycle	Type and number of cruise sampling event. Either "Stn_n" or "Cycle_n". A transect of stations was sampled from 29 June to 03 July. Five quasi-Lagrangian experiments called "cycles" were conducted during the remainder of the cruise.	text	no_bcodmo_term
coccus_s	Abundance of Synechococcus abundance.	cells per milliliter (cells/mL)	coccus_s
peuk	Abundance of Photosynthetic Eukaryotes.	cells per milliliter (cells/mL)	pico_euks

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