Dataset: brooded coral larvae 1 - respiration

Dark respiration of larvae Larval respiration was measured as O2 uptake by a group of 6 swimming larvae in Wheaton vials (2 mL) that were incubated in darkness at the same temperature as the treatments (~28C) (Edmunds et al. 2011). O2 was measured using a ruthenium-based optrode (FOXY-R, 1.58 diameter, Ocean Optics) connected to a spectrophotometer (USB2000, Ocean Optics) and interfaced with a computer running Ocean Optics software (OOISensor, version 1.00.08). The optrode was two-point calibrated in a zero solution (sodium sulfite and 0.010 mol L-1 sodium tetraborate) and water-saturated air at the experimental temperature. At each sampling, four replicates of 6 larvae were placed in Wheaton vials filled with filtered seawater (1 lm, FSW) and sealed with Parafilm; these were accompanied by four control vials that were processed in an identical manner but without larvae. The O2 saturation of the water used to fill the vials was recorded using the optrode and again in each vial following incubations lasting 1.5-2.0 h. The incubations were completed in a temperature-regulated bath (±0.1C, Hipoint, models LC-06), and the vials were inverted prior to measuring O2 saturation; larval motility during the incubations prevented the formation of boundary layers. O2 saturation was converted to concentration using gas tables [N. Ramsing and J. Gundersen at http://www.unisense.com (based on Garcia and Gordon 1992)], and the temperature and salinity of the seawater, and the change in O2 concentration converted to nmol O2 min-1 larva-1 after adjusting for control O2 fluxes.