Table of Contents

• Dataset Description
  • Methods & Sampling
  • Data Processing Description
• Data Files
• Parameters
• Instruments
• Deployments
• Project Information
• Program Information
• Funding

ARISA relative abundances (bacterial community structure) and environmental measurements. All measurements were taken from August 2000 – January 2011 at five depths from the San Pedro Channel 5m, the Deep Chlorophyll Maximum (DCM or CMAX), 150m, 500m, 890m. Additional data will be added periodically.

Related Dataset:
SPOT environmental data
ARISA Bin Taxonomy
SPOT cruises

Water samples were collected on monthly trips to the SPOT station in the San Pedro Basin.

Cruise logs are available at: http://dornsife.usc.edu/spot/document-access/

Detailed information on the methodology (pdf), including:
    Satellite measurements
    Assigning Taxonomic Identities to ARISA peaks
    Environmental parameter variability
    Seasonal variability of microbial community structure
    Mantel test approach
    Interannual variability of microbial community structure
    Alpha diversity: 
        Variability between depths
        Relation to season
        Relation to community similarity between depths
        Relation to community change
        Environmental parameters and community structure: Mantel tests
    Temporal dynamics of microbial taxa over time
        Transformations
        Taxonomic Groups
        OTUs

Relevent References:

Beman JM, Steele JA, Fuhrman JA. (2011). Co-occurrence patterns for abundant marine archaeal and bacterial lineages in the deep chlorophyll maximum of coastal California. ISME J 5: 1077-1085.

* Cram JA, Chow C-ET, Sachdeva R, et al. (2014) Seasonal and interannual variability of the marine bacterioplankton community throughout the water column over ten years. The ISME Journal. doi: 10.1038/ismej.2014.153.

Frouin R, Franz BA, Werdell PJ (2003) The SeaWiFS PAR product. Algorithm updates for the fourth SeaWiFS data reprocessing 46-50.

Fuhrman J, Azam F (1982) Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters - evaluation and field results. marine biology 66:109-120.

Kirchman D, K’nees E, Hodson R (1985) Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems. Appl Environ Microbiol 49:599-607.

Morel A, Gentili B (2009) A simple band ratio technique to quantify the colored dissolved and detrital organic material from ocean color remotely sensed data. Remote Sensing of Environment 113:998-1011. doi: 10.1016/j.rse.2009.01.008

Noble RT, Fuhrman JA. (1998). Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria. Aquat. Microb. Ecol 14: 113-118.

Parsons TR (1984) A manual of chemical and biological methods for seawater analysis, 1st ed. Pergamon Press, Oxford [Oxfordshire]; New York

Patel A, Noble RT, Steele JA, Schwalbach MS, Hewson I, Fuhrman JA. (2007). Virus and prokaryote enumeration from planktonic aquatic environments by
epifluorescence microscopy with SYBR Green I. Nat Protoc 2: 269-276.

Stramski D, Reynolds RA, Babin M, et al. (2008) Relationships between the surface concentration of particulate organic carbon and optical properties in the eastern South Pacific and eastern Atlantic Oceans. Biogeosciences 5:171-201.

BCO-DMO Processing:

- split environmental and biological data into separate datasets to reduce overall size of each dataset
- added conventional header with dataset name, PI name, version date
- renamed parameters to BCO-DMO standard or more descriptive terms
- moved columns amoA through bcsim89 before the ARISA_# columns
- transformed ARISA_####.### columns to rows with new column 'arisa_frag' for the arisa name and 'rel_abund' for the values
- replaced NA with nd
- reformated data from m/d/yyyy to yyyy-mm-dd
- changed year from 1 or 2 digits to 4 digits
- added lat, lon, cruise_id, cruise_id2
- missing depth data added from CTD logs where available

Description from NSF award abstract:
Bacteria, Archaea, and Protists dominate global elemental cycling and are immensely diverse genetically, taxonomically, and functionally. Yet the extent of marine microbial diversity, its patterns, and relationships among genetic, taxonomic, and functional diversity are very poorly characterized, even though the ocean covers 70% of the planet's surface. Among the least well known variables is the effect of human impacts on native marine microbial systems, although it is recognized that impacted systems are more prone to events like harmful algal blooms. Knowledge of these relationships and impacts are necessary to anticipate the responses of biota to global changes and feedback mechanisms that may alter the extents, rates, and even pathways of such changes. This project will expand upon an existing NSF-funded 10+-year monthly ocean time series (Microbial Observatory) that has focused on a single site midway between Los Angeles and Santa Catalina Island, to also include quarterly sampling adjacent to the impacted LA Harbor region to the barely-impacted Catalina coast. USC already runs facilities in LA Harbor and Catalina, with daily boats between (no cost). Measurements include (1) Genetic diversity: high throughput DNA sequences of "housekeeping" and functional genes. (2) Taxonomic diversity: high throughput tag sequences of small subunit ribosomal RNA genes, flow cytometry, automated image analysis (3) Functional Diversity: (a) Functional measurements (carbon fixation and respiration rates, microbial growth and grazing rates, cell size, morphology, and biomass variations), (b) distribution and expression of particular target functional genes involved with processes central to the cycles of carbon, nitrogen, and sulfur, (c) exploratory metatranscriptomics to explore functionalities that were not anticipated. (4) Integrating these: Multivariate statistical and network approaches including newly developed techniques (e.g. Bayesian networks to examine cause-effect relationships), and high speed computational approaches to assess the relationships among the genetic, taxonomic, and functional aspects of biodiversity observed. The PIs will also examine the collected data for signatures and specific effects (on organism identity and functions) associated with human impacted harbor site vs. the relatively pristine one.

The PIs will use network and time series analysis, along with other statistical tools to integrate "classical" microbial and oceanographic rate process measurements, flow cytometric and microscopic characterizations of communities, along with targeted as well as untargeted metagenomics and metatranscriptomics to relate genetic and taxonomic diversity with specific functions (at organismal, food web, and system levels). For example, they should be able to determine how different variants of particular taxa (e.g. at resolution levels ranging from what might be considered near the subspecies to genus levels) would differ in their association with particular measured functions, functional genes, or particular other taxa - or they might see how particular clusters of related organisms behave similarly or differently in their associations. This project offers an unprecedented and potentially transformative opportunity to combine and integrate measurements of genetic, taxonomic, and functional diversity along with direct measurements of system function in a well studied marine system that includes a gradient from one of the world's busiest harbors to a largely pristine ocean habitat. Far beyond just describing the distributions of organisms and functions (itself a necessary first step), they will specifically link spatial and temporal variations in a variety of functions with variations in genetic and taxonomic community composition.

Description from NSF award abstract:
Marine microbes are tremendously abundant and are major players and driving forces in global biogeochemical cycles of carbon, nitrogen, phosphorus, and iron. We learned over the past two decades that viruses are pervasive elements in marine systems, with significant ecological, biogeochemical, genetic, and evolutionary effects on cellular marine organisms, but we have remarkably little information about the dynamics of marine viral community structure and how it relates to the community structure of their hosts (largely bacteria and phytoplankton). Such information is critical for developing proper conceptual and practical models of the roles of viruses and how these change over time and space. The goals of this project are:
(1) primarily, to characterize a significant subset of the natural virus community and its dynamics, along with bacterial host communities, as they change over daily to monthly time scales at the USC well-studied marine Microbial Observatory site (midway between Los Angeles and Santa Catalina Island), testing hypotheses regarding repeating patterns, host range effects, and taxa-time relationships, and
(2) secondarily, to incorporate these viruses into microbial association networks by statistically connecting particular types of viruses to specific potential hosts.

Approaches for this study include:
(a) nested daily, weekly, and monthly collection of bacteria and viruses for nucleic acid samples,
(b) amplification of conserved genes, as proxy phylogenetic markers, from a few moderately-well-characterized broad viral groups previously readily found in seawater (i.e. the T4-like myoviruses, T7-like podoviruses), as well as bacterial rRNA genes,
(c) extensive sequencing, after screening by community fingerprinting, from the mixed amplified products,
(d) binning of the sequences or fingerprint fragments into operational taxonomic units (OTUs) at different levels of resolution,
(e) evaluation of the results with statistical approaches to examine temporal patterns, relationships (including time-lagged ones) with other viral OTUs, bacteria, protists (monthly only), and environmental parameters,
(f) incorporating the viral OTUs mathematically into microbial association networks.

Data on environmental parameters, bacteria, and protists are already being collected monthly for an existing Microbial Observatory, so the viral work is complementary to this project, providing a major value-added component. Similarly, this project will add selected daily and weekly microbial data to the Microbial Observatory. Data from the literature and from the PI's preliminary results show they have the technology and capability to meet the first goal, and to our knowledge this would be the first such data set of its scope and kind. The investigators have already published in 2006 that the bacterial communities at the 5m depth of this site show a predictable repeating annual cycle in bacterial community composition, so the expectation of a predictable repeating viral community is not unreasonable. They also have some preliminary data showing some repeated viral occurrences. The second goal requires that there are indeed significant statistical relationships between the viruses and other measured parameters, which the PI anticipates to be the case, but of course cannot predict; if they cannot be demonstrated, this result itself would be informative and would constrain the possible modes of microbial/viral interactions.

(adapted from the NSF Synopsis of Program)
Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program.  [MORE from NSF]

The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes.