Dataset: 3a_gut evacuation time

Name: Experimental results describing copepod gut evacuation time from species collected in the San Francisco estuary during 2013
Published: 2015-01-15
Keywords: biota, oceans

Cite This Dataset

Project:

Feeding and food limitation in copepod nauplii, the neglected life stage (food limitation in copepod nauplii)

Principal Investigator:

William Kimmerer (San Francisco State University, SFSU)

Co-Principal Investigator:

Sarah Cohen (San Francisco State University, SFSU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2015-01-15

Version Date:

2015-01-15

Validated:

Yes

Current State:

Final no updates expected

Experimental results describing copepod gut evacuation time from species collected in the San Francisco estuary during 2013

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Description

We determined the minimum time necessary for copepods to evacuate their guts.

Related Reference:

Vogt, R.A., T.R. Ignoffo, L.J. Sullivan, J. Herndon, J.H. Stillman, and W. Kimmerer. 2013. Feeding capabilities and limitations in the nauplii of two pelagic estuarine copepods, Pseudodiaptomus marinus and Oithona davisae. Limnology and Oceanography 58: 2145-2157.

Methods & Sampling

Copepods may have elevated feeding rates after being starved for as little as 6 h before they are provided with food (Tiselius 1998). Groups of 10 adult female P. marinus were sorted from laboratory cultures and pipetted into 500 mL Nalgene bottles containing GF/F filtered seawater, maintained at 19°C in a constant temperature room. Copepods were sampled at 10 min intervals up to 180 min, and their extracts were measured on the microplate reader as described in Vogt (2013). Most of the gut pigment was depurated within the first 30 min of incubation in filtered water, and no further depuration occurred after 60 min. Three hour starvation periods were chosen to account for potential differences in individual variability in depuration rate.

Data Processing Description

Raw data was plotted to determine gut fluorescence in raw units, subtracting background fluorescence from copepods with empty guts (background).

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard

Data Files

File
3a_Pmar_evac.csv (Comma Separated Values (.csv), 745 bytes) MD5:8939edfcf4f221d0244b5192ee2cb9ce Primary data file for dataset ID 546506

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-0929075

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Kimmerer_2013		2009-09-01	SFSU RTC	William Kimmerer (Principal Investigator)	data info

Instruments

Fluorometer

Supplied Description:

Turner 10AU

Instrument Type

Generic Name: Fluorometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/113/

Generic Description:

A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.

plate reader

Supplied Name:

Supplied Description:

Tecan Infinite F200 or Biotek Synergy 2 microplate reader was used for each analysis. Each microplate reader contained a 430/20 EX, 680/20 EMfilter pair for Chl a.

Instrument Type

Generic Name: plate reader

Generic Description:

Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.

Spectrophotometer

Supplied Description:

Agilent 8453 spectrophotometer (Agilent Technologies)

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
replicate	replicate number	unitless	replicate
time	time elapsed	minutes	time_elapsed
fluorescence	fluorescence value	relative fluorescence units??	flr

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Database

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Dataset: 3a_gut evacuation time