| Contributors | Affiliation | Role |
|---|---|---|
| DiTullio, Giacomo | Grice Marine Laboratory - College of Charleston (GML-CoC) | Principal Investigator, Contact |
| Cooper, Emily | Grice Marine Laboratory - College of Charleston (GML-CoC) | Contact |
| Gegg, Stephen R. | Woods Hole Oceanographic Institution (WHOI BCO-DMO) | BCO-DMO Data Manager |
HPLC pigment analyses of CTD collected samples from the NBP1302 cruise (Ross Sea, Antarctica).
Water samples were collected from CTD Niskin bottles. Water samples for HPLC analyses were taken from one Niskin bottle per cast-depth combination included in the dataset. Note that the niskin_sampled column indicates which Niskin bottle the HPLC sample was taken from. The niskins_fired column indicates all Niskin bottle numbers fired at the specified depth, though the HPLC sample was taken from only one of those bottles.
Chlorophyll and accessory pigment composition was analyzed by high performance liquid chromatography (HPLC; Agilent 1100). Culture aliquots were filtered on Whatmann GF/F filters, flash frozen in liquid nitrogen, and stored at -80°C until analysis. Just prior to analysis, pigments were extracted overnight in acetone at -20°C. The following day extracted pigments were centrifuged and measured using a gradient elution method (DiTullio and Geesey, 2003), a modification of the Zapata et al 2000 method. Chromatographic separation was performed using a Waters C8 symmetry column, photodiode array and fluorescence detectors. The internal standard, β-Apo-8-carotenal-trans standard (Fluka Chemical Corp., USA) was added to extracted pigments as a peak reference. Individual pigment peaks were quantified with Chemstation software (revision B.03.01, Agilent) and our pigment action spectra library calibrated using pigment standards from DHI LABS (Hoersholm, Denmark) and in-house purifications of non-commercially available pigments. Coefficient of variation among replicate HPLC injections is < 3% and our limit of detection is approximately 1 ng L-1.
Response factors for pigments were performed using dilutions of calibration standards. Full details of data processing and methods used can be found in:
DiTullio, G. R. & Geesey, M. E. (2002) Photosynthetic pigments in marine algae and bacteria. In: BITTON, G. (ed.) The Encyclopedia of Environmental Microbiology. New York: John Wiley & Sons Inc.
BCO-DMO Processing Notes:
- Generated from original .xlsx file "NPB1302 BCO-DMO.xls, sheet: CTD Final" contributed by Emily Cooper
- Parameter names edited to conform to BCO-DMO naming convention found at Choosing Parameter Name
- Blanks (missing data) replaced with "nd" meaning "no data";
- Date reformmated to YYYYMMDD
- Time reformatted to HHMM
- Added ISO_DateTime_UTC column from original DATE and TIME fields
- Lat/Lon whole degs and minutes removed (decimal degrees preserved)
- Original Lat/Lon position for CTD-135 corrected using cruise track navigation
| File |
|---|
HPLC_Pigments_CTD.csv (Comma Separated Values (.csv), 58.11 KB) MD5:3a4655809a94db950d5b1225d7a2f574 Primary data file for dataset ID 558908 |
| Parameter | Description | Units |
| Cast | CTD Cast Identifier | text |
| Date | Date (GMT) | YYYYMMDD |
| Time | Time (GMT) | HHMM |
| ISO_Date_Time | Date/Time (GMT (ISO formatted) | YYYY-MM-DDTHH:MM:SS[.xx]Z |
| Latitude | Latitude (South is negative) | decimal degrees |
| Longitude | Longitude (West is negative) | decimal degrees |
| Depth | Sample depth | meters |
| Niskin | Niskin Bottle Number | integer |
| Sample_Num | Sample Number | integer |
| Filt_vol | Volume Filtered | liters |
| Chl_C3 | Chlorophyll c3 | ng/L |
| Chl_lide | Chlorophyllide | ng/L |
| MgDvp | Magnesium-2;4-divinyl | ng/L |
| Chl_C2 | Chlorophyll c2 | ng/L |
| Chl_C1 | Chlorophyll c1 | ng/L |
| Peridinin | Peridinin | ng/L |
| Ph_ide | Pheophorbide a | ng/L |
| But19 | 19'-butanoyloxyfucoxanthin | ng/L |
| Fuco | Fucoxanthin | ng/L |
| Neo | Neoxanthin | ng/L |
| Prasino | Prasinoxanthin | ng/L |
| Viola | Violaxanthin | ng/L |
| Hex19 | 19'-hexanoyloxyfucoxanthin | ng/L |
| DD | Diadinoxanthin | ng/L |
| cis_Fuco | cis-Fucoxanthin | ng/L |
| Allo | Alloxanthin | ng/L |
| Diato | Diatoxanthin | ng/L |
| Monad | Monadoxanthin | ng/L |
| Zeax | Zeaxanthin | ng/L |
| Lutein | Lutein | ng/L |
| Croco | Crocoxanthin | ng/L |
| Chl_b | Chlorophyll b | ng/L |
| Chlorophyll_c2_MGDG | Chlorophyll c2 MGDG | ng/L |
| Chlorophyll_a_allomer | Chlorophyll a allomer | ng/L |
| DV_Chl_a | Divinyl chlorphyll a | ng/L |
| Chl_a | Chlorophyll a | ng/L |
| Ph_tin | Phaeophytin a | ng/L |
| a_Car | Alpha-carotene | ng/L |
| b_Car | Beta-carotene | ng/L |
| Total_Chl_a | Total Chlorophyll a | ng/L |
| Dataset-specific Instrument Name | Sea-Bird 911+ CTD |
| Generic Instrument Name | CTD Sea-Bird SBE 911plus |
| Dataset-specific Description | Nathaniel B. Palmer Systems and Specifications |
| Generic Instrument Description | The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics |
| Dataset-specific Instrument Name | Trimble GPS - PCODE |
| Generic Instrument Name | Global Positioning System Receiver |
| Generic Instrument Description | The Global Positioning System (GPS) is a U.S. space-based radionavigation system that provides reliable positioning, navigation, and timing services to civilian users on a continuous worldwide basis. The U.S. Air Force develops, maintains, and operates the space and control segments of the NAVSTAR GPS transmitter system. Ships use a variety of receivers (e.g. Trimble and Ashtech) to interpret the GPS signal and determine accurate latitude and longitude. |
| Dataset-specific Instrument Name | HPLC |
| Generic Instrument Name | High-Performance Liquid Chromatograph |
| Dataset-specific Description | Chlorophyll and accessory pigment composition was analyzed by high performance liquid chromatography (HPLC; Agilent 1100). |
| Generic Instrument Description | A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. |
| Dataset-specific Instrument Name | |
| Generic Instrument Name | Niskin bottle |
| Generic Instrument Description | A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. |
| Website | |
| Platform | RVIB Nathaniel B. Palmer |
| Report | |
| Start Date | 2013-02-12 |
| End Date | 2013-04-05 |
| Description | Ross Sea, Antarctica (53 days)
RVIB Nathaniel B. Palmer : February-April 2013
McMurdo Station, Antarctica - Punta Arenas, Chile
Project Title: “TRacing the fate of Algal Carbon Export in the Ross Sea” (TRACERS)
Chief Scientist: Dennis Hansell, UM-RSMAS
Project Description: The research focus of this cruise was to investigate the biogeochemistry associated after a phytoplankton bloom at the end of the Antarctic Austral Summer. I helped analyze and coordinate analyses of nutrients (silicic acid, phosphate, and nitrate) and collect samples for dissolved organic carbon (DOC).
Note R2R Link takes user to Marine Geoscience Data System (MGDS):
NBP1302
Nathaniel B. Palmer Systems and Specifications |
Sinking particles are a major element of the biological pump and they are commonly assigned to two fates: mineralization in the water column and accumulation at the seafloor. However, there is another fate of export hidden within the vertical decline of carbon, the transformation of sinking organic matter to fine suspended and/or dissolved organic fractions. This process has been suggested but has rarely been observed or quantified. As a result, it is presumed that the solubilized fraction is largely mineralized over short time scales. However, global ocean surveys of dissolved organic carbon are demonstrating a significant water column accumulation of organic matter under high productivity environments. This proposal will investigate the transformation of organic particles from sinking to solubilized phases of the export flux in the Ross Sea. The Ross Sea experiences high export particle production, low dissolved organic carbon export with overturning circulation, and the area has a predictable succession of production and export events. In addition, the basin is shallow (< 1000 m) so the products the PIs will target are relatively concentrated. To address the proposed hypothesis, the PIs will use both well-established and novel biochemical and optical measures of export production and its fate. The outcomes of this work will help researchers close the carbon budget in the Ross Sea.
| Funding Source | Award |
|---|---|
| NSF Division of Polar Programs (NSF PLR) |