Dataset: Prochlorococcus inorganic carbon fluxes

Name: Experimental results on inorganic carbon fluxes of Prochlorococcus MED4 cultured under high and low CO2 concentrations (OA phytoplankton physiology project)
Published: 2015-07-29
Keywords: biota, oceans

Cite This Dataset

Project:

Effects of pCO2 and pH on Photosynthesis, Respiration and Growth in Marine Phytoplankton (OA_phytoplankton_physiology)

Program:

Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Ocean Carbon and Biogeochemistry (OCB)

Principal Investigator:

Brian M. Hopkinson (University of Georgia, UGA)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

2015-07-29

Version Date:

2015-07-29

Validated:

Yes

Current State:

Final no updates expected

Experimental results on inorganic carbon fluxes of Prochlorococcus MED4 cultured under high and low CO2 concentrations (OA phytoplankton physiology project)

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Description

Prochlorococcus MED4 was acclimated to low (150 ppm) or high (1000 ppm) CO2. The cultures were then concentrated and placed in the chamber of a membrane inlet mass spectrometer (MIMS; Pfieffer QMS220). Dissolved inorganic carbon (DIC) was gradually added to the culture and inorganic carbon fluxes were measured using MIMS as described in Hopkinson et al. 2014.

Relevant Reference:

Hopkinson, B.M., J.N. Young, A. L. Tansik, and B. J. Binder. 2014. The minimal CO2-concentrating mechanism of Prochlorococcus spp. MED4 is effective and efficient. Plant Physiology 166: 2205-2217.

Data Processing Description

The data represent averages from experiments that were done in at least triplicate.

BCO-DMO Processing:

- added conventional header with dataset name, PI name, version date, reference information
- renamed parameters to BCO-DMO standard

Data Files

File
ProCCM.csv (Comma Separated Values (.csv), 1.04 KB) MD5:40981ce58e9272e4ce27909db48e4f14 Primary data file for dataset ID 563152

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Emerging Frontiers Division	EF-1041034

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Hopkinson_lab		2010-09-01	Univ_Georgia	Brian M. Hopkinson (Principal Investigator)	data info

Instruments

Mass Spectrometer

Supplied Name: membrane inlet mass spectrometer (MIMS)

Supplied Description:

Membrane Inlet Mass Spectrometer (MIMS; Pfieffer QMS220)

Instrument Type

Generic Name: Mass Spectrometer

Acronym: Mass Spec

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
DIC_assay	Dissolved inorganic carbon (DIC) concentrations in the assay solution	micromolar	DIC
CO2_culture	Culture CO2 concentrations	parts per million	pCO2
photosyn_net	Net photosynthesis	mol/cell/sec	no_bcodmo_term
respiration	Respiration	mol/cell/sec	dark_O2_respir
CO2_flux	CO2 flux	mol/cell/sec	no_bcodmo_term
HCO3_flux	HCO3- flux	mol/cell/sec	no_bcodmo_term
DIC_internal	DIC concentrations inside the cell	millimolar	DIC

Status message

Database

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Dataset: Prochlorococcus inorganic carbon fluxes