Dataset: A. pisonii: egg size

Name: Comparing egg size in Aratus pisonii populations from mangrove and salt marsh habitats in South Eastern US mangrove forests during 2013 (Variation in Metabolic Processes project)
Published: 2016-03-01
Keywords: biota, oceans

Cite This Dataset

Spatial Extent: N:29.86961 E:-80.32683 S:27.55045 W:-81.27338

Project:

Linking Variation in Metabolic Processes as a Key to Prediction (Variation in Metabolic Processes)

Principal Investigator:

Dr Blaine D. Griffen (University of South Carolina)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2016-03-01

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Comparing egg size in Aratus pisonii populations from mangrove and salt marsh habitats in South Eastern US mangrove forests during 2013 (Variation in Metabolic Processes project)

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Description

This data is part of a project exploring the impact of climate-change induced range expansion on traditional ecogeographic patterns of life history. Data were collected June-August 2013.

Related Reference:
Riley ME, Griffen BD (2017) Habitat-specific differences alter traditional biogeographic patterns of life history in a climate-change induced range expansion. PLOS One 12(5):e0176263.

Methods & Sampling

We collected ovigerous Aratus pisonii from mixed marsh habitats (Avalon State Park, FL) and mangrove habitats (Anastasia State Park, FL). We gently removed a small portion (<1/3) of each female’s egg mass from the pleopods with forceps within 24 hours of collection from the field. We placed eggs into filtered sea water (~31 ppt) and visually examined under a Leica M205C dissecting microscope at 80X to determine egg developmental stage. For females carrying recently extruded, non-eyed eggs (mangroves n= 10, mixed marsh n=15), which are reflective of initial maternal investment in quantity of egg yolk, we photographed a subsample of the removed eggs (n=20-30) using a Jenoptik ProgRes C14+ camera attached to the microscope and ProgRes CapturePro v2.8.0 software. We then determined egg size by randomly selecting 10 eggs from the photographed egg mass and measuring the circular area of each egg with imageJ software. We used the measurements of circular area to determine the mean egg volume for each female.

Data Processing Description

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date
- renamed parameters to BCO-DMO standard
- replaced spaces with underscores; removed commas
- added latitude and longitude for mapping purposes
- sorted data by habitat

Data Files

File
Apisonii_eggsize.csv (Comma Separated Values (.csv), 1.66 KB) MD5:ac9454a81f94c9613ef82a863f6fc742 Primary data file for dataset ID 639282

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1129166

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Griffen_mangroves		2013-06-01	Univ_S_Carolina	Dr Blaine D. Griffen (Principal Investigator)	data info

Instruments

Camera

Supplied Name:

Supplied Description:

Jenoptik ProgRes C14+ camera attached to the microscope

Instrument Type

Generic Name: Camera

Acronym: camera

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/311/

Generic Description:

All types of photographic equipment including stills, video, film and digital systems.

Microscope - Optical

Supplied Name:

Supplied Description:

Leica M205C dissecting microscope

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
habitat	Dominant habitat: mangrove forest; marsh; mixed marsh	unitless	site_descrip
site	Site identification	unitless	site
lat	Latitude; north is positive	decimal degrees	lat
lon	Longitude; east is positive	decimal degrees	lon
crab	individual crab identification	unitless	sample
carap_width	Carapace width	millimeters	width
egg_vol	mean egg volume	cubic centimeters	volume

Status message

Database

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Dataset: A. pisonii: egg size