Dataset: Laboratory results containing transcripts of four cultivated marine bacteria from 2014-2015 (EAGER Respiration project)

This dataset has not been validatedPreliminary and in progressVersion 2016.03.03 (2016-03-03)Dataset Type:experimental

Principal Investigator: Dr Matthew T Cottrell (University of Delaware)

BCO-DMO Data Manager: Danie Kinkade (Woods Hole Oceanographic Institution)


Project: Coordination of respiratory gene transcription and respiration in cultivated marine bacteria (EAGER Respiration)

The RNA-seq data have been deposited with NCBI under GenBank Accession Number PRJNA276979.

Bacteria respond to stimuli in the environment using transcriptional control, but this may not be the case for most marine bacteria having small, streamlined genomes. Candidatus Pelagibacter ubique, a cultivated representative of the SAR11 clade, which is the most abundant clade in the oceans 4, has a small, streamlined genome and possesses an unusually small number of transcriptional regulators. This observation leads to the hypothesis that transcriptional control is low in Pelagibacter and limits its response to environmental conditions. However, the extent of transcriptional control in Pelagibacter is unknown. Overall design: Bacteria were grown in batch culture and sampled twice during the initial, rapid phase of exponential growth and twice during the phase of slower growth that followed.  Transcripts deposited here were assessed using RNA-seq.


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